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2003 Fiscal Year Final Research Report Summary

CROSS-TALK OF MEMBRANE LIPID SIGNALING IN CELL DEATH AND SURVIVAL

Research Project

Project/Area Number 14370064
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionGIFU INTERNATIONAL INSTITUTE OF BIOTECHNOLOGY

Principal Investigator

NOZAWA Yoshinori  GIFU INTERNATIONAL INSTITUTE OF BIOTECHNOLOGY, DIRECTOR・CHIEF, 所長 (10021362)

Co-Investigator(Kenkyū-buntansha) BANNO Yoshiko  GIFU UNIVERSITY SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR, 医学部, 助教授 (50116852)
TANAKA Masashi  GIFU INTERNATIONAL INSTITUTE OF BIOTECHNOLOGY, CHIEF, 部長 (60155166)
AKAO Yukihiro  GIFU INTERNATIONAL INSTITUTE OF BIOTECHNOLOGY, CHIEF, 部長 (00222505)
OHGUCHI Kenji  GIFU INTERNATIONAL INSTITUTE OF BIOTECHNOLOGY, RESERCHER, 研究員 (80359257)
Project Period (FY) 2002 – 2003
KeywordsAPOPTOSIS / SURVAIVAL / Pyk2 / PHOSPHOLIPASE D / AKT / PHOSPHATIDYLINOSITOL 3-KINASE / ERK / スフィンゴシンキナーゼ
Research Abstract

We have previously proposed the notion that phospholipase D (PLD) plays an important role in the anti-apototic or survival signaling pathway. When PC12 cells were exposed to the oxidative stress (H_2O_2), the PLD activity was found to greatly increase at the early stage. It was demonstrated that the Pyk2 which acts as a scarffold and Src kinase are colsely associated with the H_2O_2-induced PLD activation. In addition, Pyk2 was found to be physically interacted with PLD as inferred by co-immunoprecipitation. Thus it was concluded that the Pyk2/Src/Akt pathway is of primary importance for the survival signaling at the early phase after the H_2O_2 stress.
On the other hand, it has been known that sphingosine kinase produces sphingosine-1-phosphate whth exerts a survival signaling by its specific receptor activation (S1Ps). Furthermore, it was shown that the sphingosine kinase was much higher in the activity and protein expression level in camptothecin (CPT)-resistant PC3 cells as compared to CPT-sensitive LNCaP cells of human prostate cancer cells. Also, we have obtained the unexpected, interesting finding that CPT treatment enhanced the SPHK activity in a time-dependent manner, but the underlying mechanism remains to be defined. This can explain at least in part the CPT-resistance of PC3 cells.

  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Nozawa, Y.et al.: "Roles of phospholipase D in apoptosis and pro-survival."Biochim.Biophys.Acta. 55973. 1-10 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nozawa, Y.et al.: "Involvement of phospholipase D in insulin-like growth factor induced activation of extracellular signal-regulated kinase, but not phosphatydylinositol 3-kinase or Akt, in Chinese hamster ovary cell."Biochem.J.. 369. 363-368 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Banno, Y.et al.: "Hydrogen peroxide-induced phospholipase D activation and its PKC dependence are modulated by pH changes in PC12 cells."Biochem.Biophys.Res.Commun.. 312. 1087-1093 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamada, M.et al.: "Overexpression of PLD prevents actinomycin D-induced apoptosis through potentiation of PI3K signaling pathways in Chinese hamster ovary cells."Biochem.J.. 378. 1-8 (2004)

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      「研究成果報告書概要(和文)」より
  • [Publications] Ohguchi, K.et al.: "Involvement of phospholipase D1 in melanogenesis of mouse B16 melanoma cell."J.Bio.Chem.. 279. 3408-3412 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nozawa, T.: "A thematic series on lipid signaling : Prologue"J.Biochem.. 131. 283-284 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akao, Y.: "Ceramide accumulation is independent of camptothecin-induced apoptosis in prostate cancer LNCaP cells"Biochem.Biophys.Res.Commun.. 294. 363-370 (2002)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Wang, S.: "Two forms of membrane-bound sphingosine kinase in tetrahymena and activity changes during growth and the cell cycle"J.Eukaryot.Microbiol.. 49(4). 305-311 (2002)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Tan, Z.: "Involvement of the mitogen-activated protein kinase family in tetracaine-induced PC12 cell death"Anesthesiology. 96. 1191-1201 (2002)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Chen, J.: "The inhibitory effect of local anesthetics on bradykinin-induced phospholipase D activation in rat pheochromocytoma PC12 cells"Anesth.Analg.. 95(1). 88-97 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Osawa, Y.: "Tumor necrosis factor alpha-induced interleukin-8 production via NF-κB and phosphatidylinositol 3-kinase/Akt pathways inhibits cell apoptosis in human hepatocytes"Infect.Immunity. 70(11). 6294-9301 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nozawa, Y.: "Roles of phospholipase D in apoptosis and pro-survival"Biochim.Biophys.Acta(Molecular Cell Biology) Special issue "Lipids in Apoptosis". 55973. 1-10 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Banno, Y.: "Involvement of phospholipase D in insulin-like growth factor-I-induced activation of intracellular signal-regulated kinase, but not phosphatydylinositol 3-kinase or Akt, in Chinese hamster ovary cells"Biochem.J.. 369. 363-368 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Osawa, Y.: "Expression of the NF-κB target gene x-ray-inducible immediate early response factor-1 short enhances TNF-α-induced hepatocyte apoptosis by inhibiting Akt activation"J.Immunol.. 170. 4053-4060 (2003)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Sagawa, N.: "Gelsolin suppresses tumorigenicity through Inhibiting PKC activation in a human lung cancer cell line, PC10"Brt.J.Cancer. 88. 609-612 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Banno, Y.: "Hydrogen peroxide-induced phospholipase D activation and its PKC dependence are modulated by pH changes in PC12 cells"Biochemi.Biophys.Res.Commun.. 312. 1087-1093 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Murate, T.: "Regulation of sphingosine kinase 1 gene expression by protein kinase C in a human leukemia cell line, MEG-01"Biochim.Biophys.Acta.. 1635. 104-116 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamada, M.: "Overexpression of phospholipase D prevents actinomycin D-induced apoptosis through potentiation of phosphoinositide 3-kinase signaling pathways in Chinese hamster ovary cells"Biochem.J.. 378. 1-8 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohguchi, K.: "Involvement of phospholipase D1 in melanogenesis of mouse B16 melanoma cell"J.Biol.Chem.. 279. 3408-3412 (2004)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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