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2004 Fiscal Year Final Research Report Summary

Analysis of familial and multiple GIST

Research Project

Project/Area Number 14370076
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Experimental pathology
Research InstitutionHyogo College of Medicine (2004)
Osaka University (2002-2003)

Principal Investigator

HIROTA Seiichi  Hyogo College of Medicine, Faculty of Medicine, Professor, 医学部, 教授 (50218856)

Project Period (FY) 2002 – 2004
KeywordsGIST / Familial and multiple GISTs / model mouse / knock-in-mouse / Imatinib / c-kit gene / PDGFR alpha / von Recklinghausen disease
Research Abstract

We found a patient with multiple GISTs(gastrointestinal stromal tumors), and examined his family to clarify whether the cause of multiple GISTs might be derived from germline c-kit mutation. Some members of his family similarly had multiple GISTs, and they had c-kit gene mutation at tyrosine kinase II domain. Proliferation of interstitial cells of Cajal(ICCs) was also seen in the patients as in the cases reported previously. The mutation was proved to be gain-of-function mutation, and is considered to be a cause of multiple GISTs in this family. This family is the first case with germline c-kit gene mutalion at at tyrosine kinase II domain.
We further examined the clonality of diffuse proliferation of ICCs in familial GIST patients. The proliferative lesion showed polyclonal nature while each GIST demonstrated to be monoclonal. We also showed that KIT activation by tyrosine kinase II domain mutation was not effectively inhibited by a selective tyrosine kinase inhibitor, Imatinib. The downstream molecules of KIT signal transduction were not also fully inhibited by Imatinib.
We investigated the cause of GISTs without c-kit gene mutation. Approximately half of GISTs without c-kit gene mutation had PDGFR alpha gene mutation. Two types of PDGFR alpha gene mutation were seen, and KIT activation by the juxtamembrane domain mutation was effectively inhibited by Imatinib but that by the tyrosine kinase II domain mutation was not. We demonstrated that regrowth of GISTs during the Imatinib treatment (development of resistant clone) was caused by an additional c-kit gene mutation to original c-kit gene mutation. Moreover, we showed that GISTs from neurofibromatosis type1 patients did not have any c-kit gene mutation.

  • Research Products

    (17 results)

All 2004 2003 2002

All Journal Article (16 results) Book (1 results)

  • [Journal Article] Late resistance to imatinib therapy associated with a second KIT mutation in metastatic gastrointestinal stromal tumour.2004

    • Author(s)
      Wakai T, Hirota S, et al.
    • Journal Title

      Br J Cancer 90

      Pages: 2059-2061

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Absence of c-kit gene mutations in gastrointestinal stromal tumours of neurofibromatosis type I patients.2004

    • Author(s)
      Kinoshita K, Hirota S, et al.
    • Journal Title

      J Pathol 202

      Pages: 80-85

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Different inhibitory effect of Imatinib on phosphorylation of MAPK and Akt, and cell proliferation in cells expressing different types of mutant PDGF receptor alpha.2004

    • Author(s)
      Ohashi A, Hirota S, et al.
    • Journal Title

      Int J Cancer 111

      Pages: 317-321

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Late resistance to imatinib therapy associated with a second KIT mutation in metastatic gastrointestinal stromal tumour.2004

    • Author(s)
      Wakai T, Kanda T, Hirota S, Ohashi A, Shirai Y, Hatakeyama K
    • Journal Title

      Br J Cancer 90

      Pages: 2059-2061

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Absence of c-kit gene mutations in gastrointestinal stromal tumours of neurofibromatosis type I patients.2004

    • Author(s)
      Kinoshita K, Hirota S, Isozaki K, Ohashi A, Nishida T, Kitamura Y, Shinomura Y, Matsuzawa Y
    • Journal Title

      J Pathol 202

      Pages: 80-85

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Different inhibitory effect of Imatinib on phosphorylation of MAPK and Akt, and cell proliferation in cells expressing different types of mutant PDGF receptor alpha.2004

    • Author(s)
      Ohashi A, Kinoshita K, Isozaki K, Nishida T, Shinomura Y, Kitamura Y, Hirota S
    • Journal Title

      Int J Cancer 111

      Pages: 317-321

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Gain-of-function mutations of platelet-derived growth factor receptor alpha gene in gastrointestinal stromal tumors.2003

    • Author(s)
      Hirota S, et al.
    • Journal Title

      Gastroenterology 125

      Pages: 660-667

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Familial gastrointestinial stromal tumors associated with dysphagia and novel type germline mutation of KIT gene.2003

    • Author(s)
      Hirota S, et al.
    • Journal Title

      Gastroenterology 122

      Pages: 1493-1499

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Gain-of-function mutations of platelet-derived growth factor receptor alpha gene in gastrointestinal stromal tumors.2003

    • Author(s)
      Hirota S, Ohashi A, Nishida T, Isozaki K, Kinoshita K, Shinomura Y, Kitamura Y
    • Journal Title

      Gastroenterology 125

      Pages: 660-667

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Endoscopic ultrasonography guided fine needle aspiration biopsy in follow-up patients with gastrointestinal stromal tumors.2003

    • Author(s)
      Kinoshita K, Isozaki K, Tsutsui S, Kitamura S, Hiraoka S, Watabe K, Nakahara M, Nagasawa Y, Kiyohara T, Miyazaki Y, Hirota S, Nishida T, Shinomura Y, Matsuzawa Y
    • Journal Title

      Eur J Gastroenterol Hepatol 15

      Pages: 1189-1193

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Imatinib inhibits various types of activating mutant KIT found in gastrointestinal stromal tumors.2003

    • Author(s)
      Chen H, Isozaki K, Kinoshita K, Ohashi A, Shinomura Y, Matsuzawa Y, Kitamura Y, Hirota S
    • Journal Title

      Int J Cancer 105

      Pages: 130-135

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] C-kit gene mutation at exon 17 or 13 is very rare in sporadic gastrointestinal stromal tumors.2003

    • Author(s)
      Kinoshita K, Isozaki K, Hirota S, Nishida T, Chen H, Nakahara M, Nagasawa Y, Ohashi A, Shinomura Y, Kitamura Y, Matsuzawa Y
    • Journal Title

      J Gastroenterol Hepatol 18

      Pages: 147-151

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Polyclonal nature of diffuse proliferation of interstitial cells of Cajal in patients with familial and multiple gastrointestinal stromal tumours.2002

    • Author(s)
      Chen H, Hirota S, et al.
    • Journal Title

      Gut 51

      Pages: 793-796

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Improvement of psoriasis during imatinib therapy in a patient with a metastatic gastrointestinal stromal tumour.2002

    • Author(s)
      Miyagawa S, Fujimoto H, Ko S, Hirota S, Kitamura Y
    • Journal Title

      Br J Dermatol. 147

      Pages: 406-407

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Polyclonal nature of diffuse proliferation of interstitial cells of Cajal in patients with familial and multiple gastrointestinal stromal tumours.2002

    • Author(s)
      Chen H, Hirota S, Isozaki K, Sun H, Ohashi A, Kinoshita K, O'Brien P, Kapusta L, Dardick I, Obayashi T, Okazaki T, Shinomura Y, Matsuzawa Y, Kitamura Y
    • Journal Title

      Gut. 51

      Pages: 793-796

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Familial gastrointestinial stromal tumors associated with dysphagia and novel type germline mutation of KIT gene.2002

    • Author(s)
      Hirota S, Nishida T, Isozaki K, Taniguchi M, Nishikawa K, Ohashi A, Takabayashi A, Obayashi T, Okuno T, Kinoshita K, Chen H, Shinomura Y, Kitamura Y
    • Journal Title

      Gastroenterology 122

      Pages: 1493-1499

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Gastrointestinal stromal tumor(GIST) : From Pathology to Molecular target therapy2004

    • Author(s)
      Hirota S, et al.
    • Total Pages
      176
    • Publisher
      Japan Scientific Societies Press, Tokyo, and Karger, Basel
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2006-07-11  

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