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2004 Fiscal Year Final Research Report Summary

Structural analysis of envelope proteins of macrophage tropic HIV

Research Project

Project/Area Number 14370159
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionTohoku University

Principal Investigator

HATTORI Toshio  Tohoku University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (30172935)

Co-Investigator(Kenkyū-buntansha) ASHINO Yugo  Tohoku University, Hospital, Research Associate, 病院・助手 (20361082)
SANO Kunio  Tohoku University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (20192601)
Project Period (FY) 2002 – 2004
Keywordsgp41 / HIV / antibody / AIDS / vaccine
Research Abstract

To understand the mechanisms of HIV infection, we clarified that N terminus of V3 loop of BH-10 clone is responsible for its binding to the cells. We also treated the surface of H9/IIIB cells by several proteases including thrombin and analyzed the expression of functional epitomes by FACS. Thrombin treatment decreased the binding of anti-V3 mAb while enhanced the bindings of mAbs against Co-receptor binding site, a CD4 binding site and neutralizing epitope of gp41. Thrombin also enhanced fusion between H9/IIIB and MAGI cells. These findings support that blood borne inflammatory molecule thrombin activate gp 120 and enhanced the fusion.
We also evaluated monoclonal antibodies (mAbs) against the gp41 core structure such as mAbs 50.69, 98.6 and T26, by western blotting and flow cytometry. Western blotting showed mAbs 50.69 and 98.6 bound to both monomeric and oligomeric gp41 and mAb T26 exclusively bound to oligomeric gp41. We evaluated the sera from pneumocystis pneumonia patients (PCP) and long term survivors (LTS). The results revealed that PCP sera as well as LTS sera inhibited the binding of all the three mAbs, and the PCP sera inhibited mAb T26 binding efficiently than LTS. Therefore PCP patients retain competing immunity to antibodies against not only the shared epitopes of the core structure (binding sites of mAbs 50.69 and 98.6) but also against oligomeric gp41 specific epitope (binding site of mAb T26).

  • Research Products

    (8 results)

All 2005 2004

All Journal Article (8 results)

  • [Journal Article] Low antibody response against tuberculous glycolipid (TBGL) in elderly gastrectomized tuberculosis patients2005

    • Author(s)
      Toshio Hattori他5名
    • Journal Title

      The International Journal of Tuberculosis and Lung Disease (In press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Low antibody response against tuberculous glycolipid (TBGL) in elderly gastrectomized tuberculosis patients2005

    • Author(s)
      T Hattori et al.
    • Journal Title

      The International Journal of Tuberculosis and Lung Disease (In Press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Thrombin activates envelope glycoproteins of human immunodeficiency virus type 1 (HIV-1) and enhances fusion.2004

    • Author(s)
      Toshio Hattori他3名
    • Journal Title

      Microbes and Infection 6

      Pages: 414-420

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Surveillance of humoral immune responses to tuberculosis glycolipid antigen (TBGL) in TB and HIV infected individuals.2004

    • Author(s)
      Toshio Hattori他5名
    • Journal Title

      XV International AIDS Conference E710L77812

      Pages: 53-56

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The N-terminal of the V3 loop in HIV-1 gp120 is responsible for its conformation-dependent interaction with cell surface molecule(s)2004

    • Author(s)
      Toshio Hattori他4名
    • Journal Title

      AIDS Res.Hum.Retrovir. 20

      Pages: 213-218

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Thrombin activates envelope glycoproteins of human immunodeficiency virus type 1 (HIV-1) and enhances fusion.2004

    • Author(s)
      T Hattori et al.
    • Journal Title

      Microbes and Infection 6

      Pages: 414-420

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Surveillance of humoral immune responses to tuberculosis glycolipidantigen (TBGL) in TB and HIV infected individuals2004

    • Author(s)
      T Hattori et al.
    • Journal Title

      XV International AIDS Conference E710L77812

      Pages: 53-56

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The N-terminal of the V3 loop in HIV-1 gp120 is responsible for its conformation-dependent interaction with cell surface molecule(s)2004

    • Author(s)
      Toshio Hattori et al.
    • Journal Title

      AIDS Res.Hum.Retrovir. 20

      Pages: 213-218

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2006-07-11  

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