2003 Fiscal Year Final Research Report Summary
Identification of gastrointestinal epithelial stem cell and development of regeneration therapy for IBD
| Project/Area Number |
14370192
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Keio University |
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Principal Investigator |
HIBI Toshifumi Keio University, School of Medicine, Professor, 医学部, 教授 (50129623)
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| Co-Investigator(Kenkyū-buntansha) |
WATANABE Mamoru Tokyo Medical & Dental University, Dept of Medicine, Professor, 医学部, 教授 (10175127)
OKANO Hideyuki Keio University, School of Medicine, Professor, 医学部, 教授 (60160694)
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| Project Period (FY) |
2002 – 2003
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| Keywords | intestinal epithelial cell / stem cell / side population cell / musashi-1 / inflammatory bowel disease / regeneration of mucosa |
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| Research Abstract |
Epithelial renewal occurs in the crypts through a coordinated series of events involving proliferation, differentiation and migration toward the intestinal lumen. It was believed that pluripotent stem cells were located at the lower third of the crypt and generated differentiated cells, such as intestinal epithelial cells, enteroendocrine cells, paneth cells and goblet cells. However, their manner of proliferation and differentiation remains unknown because they lack specific markers and methods of purification and culture. In this study, we propose a new technique to enrich epithelial stem cells and evaluate their phenotype.
We have separated crypts and villi from adult murine small intestine, and have shown that dephosphorylated β catenin and musashi-1 was enriched in crypts fraction. Crypt epithelial cells contained increased numbers of side population (SP) cells and as compared with villous epithelial cells. In adult intestinal epithelial cells, SP cells have higher amounts of ABCG-2 as compared with main population (MP) cells. Fetal intestinal epithelial cells contained large numbers of SP cells. Dephosphorylated β catenin was enriched in SP cells as compared with MP cells.
These results suggested that intestinal stem cell could be purified using SP cell fraction in intestinal epithelial cells. Further study is needed to determine whether SP cells have functional stem cell activities in vivo and in vitro.
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Research Products
(10 results)
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[Publications] Sato T, Kanai T, Watanabe M, Sakuraba A, Okamoto S, Nakai T, Okazawa A, Inoue N, Totsuka T, Yamazaki M, Kroczek RA, Fukushima T, Ishii H, Hibi T: "Hyperexpression of Inducible Costimulator and Its Contribution on Lamina Propria T cells in Inflammatory Bowel Disease."Gastroenterology. 126. 829-839 (2004)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yamazaki M, Yajima T, Tanabe M, Fukui K, Okada E, Okamoto R, Oshima S, Nakamura T, Kanai T, Uehira M, Takeuchi T, Ishikawa H, Hibi T, Watanabe M: "Mucosal T cells expressing high levels of IL-7 receptor are potential targets for treatment of chronic colitis."J Immunol. 171. 1556-1563 (2003)
Description
「研究成果報告書概要(欧文)」より
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