• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2003 Fiscal Year Final Research Report Summary

Longterm gene therapy of xeroderma pigmentosum group A mice using HVJ-liposomes.

Research Project

Project/Area Number 14370258
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Dermatology
Research InstitutionKobe University (2003)
Kyoto University (2002)

Principal Investigator

NISHIGORI Chikako  Kobe University, Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (50198454)

Project Period (FY) 2002 – 2003
Keywordsxeroderma pigmentosum / HVJ-liposomes / gene therapy / nucleotide excision repair / skin tumor / ultraviolet light
Research Abstract

Xeroderma pigmentosum (XP) is an autosomalrecessibely inheritated disorder in which nucleotide excision repair is deficient, thus the patients with XP cause skin cancer of sun-exposed area in high frequency. Among XP complementation groups, XP complementation group A (XPA) reveals the severest clinical phenotype with neurological abnormalities and XPA is the most common type among Japanese XPA patients. The aim of this project is to challenge the gene therapy of XPA model mice in vivo with treating human XPA gene in the form of HVJ-liposomes, since one of our research goal is to establish the gene therapy for XPA patients. Two plasmids containing human XPA gene, pCAGGS-XPA or pcHA-XPA, which contains hemagultinin gene as a tag, was transfected and the repair ability was measured by unscheduled DNA syntshesis at post 24 or 48 hr UVC irradiation. Both recovered the repair efficiency in XPA mice cells. In in vivo study HVJ (Hemagluutinating Virus of Japan)-liposomes was used for introducing the plasmid containing human XPA gene into mice skin. HVJ-liposomes-XPA was injected intradermaly 24 hr before 4 kJ/m^2 of UVB irradiation or topically painted 48 hr before UVB irradiation. Unscheduled DNA synthesis of epidermal nuclei in mice injected XPA-HVJ liposmes was higher than that of control liposomes. Next we irradiated the mice with 500 J/m^2 of UVB 1-2 times per week for 3 months with or without treating HVJ-XPA-liposmes 24 or 48 hr prior to UVB irradiation. Both pcHA-XPA and pCAGGS-XPA encapsulated in HVJ-liposmes reduced the size, the number of the UV-induced tumors and elongate the survival duration. However, both treatment did not completely inhibit the tumor formation. The histology of the tumor was squamous cell carcinoma. In conclusion, the treatment of HVJ-XPA liposomes has effect on reducing the UV-induced tumors in XPA model mice.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Yumi Matsumura: "Expression of CD134 and CD ligand in lesional, and nonlesional psoriatic skin."Arch Dermatol Res. 294. 563-566 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chikako Nishigori: "Phtoageing and oxidative stress."Experimental Dermatology. 12suppl2. 18-21 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahiro Oka: "PKCd inhibits PKCa-mediated activation of phospholipase D1 in a manner independent of its protein kinase activity"Febs letter. 554. 179-183 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Atsushi Fukunaga: "SHPS-1 regulates the migration of Langerhans cells from the epidermis to draining lymph nodes."J Immunol. 172. 4091-4099 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahiro Ono: "Cutaneous Alterenariosis in an immunocompetent patient ; analysis of internal transcribed spacer region of rDNA and Brm2 of isolated Alternaria alternata."Br J Dermatol. 150. 773-775 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Chikako Nishigori: "Role of reactive oxygen species in skin carcinogenesis."Antioxidant redox signaling. 6. 561-570 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 錦織 千佳子: "最新皮膚科学大系 第16巻"中山書店. 389 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yumi, Matsumura: "Expression of CD134 and CD ligand in lesional, and nonlesional psoriatic skin."Arch Dermatol Res. 294. 563-566 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Chikako, Nishigori: "Phtoageing and oxidative stress"Experimental Dermatology. 12 suppl 2. 18-21 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiro Oka: "PKCd inhibits PKCa-mediated activation of phospholipase D1 in a manner independent of its protein kinase activity."Febs letter. 554. 179-183 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Atsushi, Fukunaga: "SHPS-1 regulates the migration of Langerhans cells from the epidermis to draining lymph nodes"J Immunol. 172. 4091-4099 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahiro, Ono: "Cutaneous Alterenariosis in an immunocompetent patient ; analysis of internal transcribed spacer region of rDNA and Brm2 of isolated Alternaria alternata"Br J Dermatol. 150. 773-775 (2004)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2005-04-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi