2003 Fiscal Year Final Research Report Summary
Clarification of response mechanism in U937 cells against heat treatment
| Project/Area Number |
14370271
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Radiation science
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| Research Institution | International University of Health and Welfare (2003)
The University of Tokyo (2002) |
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Principal Investigator |
SUZUKI Norio International University of Health and Welfare, Radiological Sciences, Professor, 保健学部, 教授 (10010050)
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| Co-Investigator(Kenkyū-buntansha) |
MORITA Akinori The University of Tokyo, Radiation Research, Assistant professor, 大学院・医学系研究科, 助手 (90334234)
ENOMOTO Atsushi The University of Tokyo, Radiation Research, Assistant professor, 大学院・医学系研究科, 助手 (20323602)
MATSUMOTO Yoshihisa The University of Tokyo, Radiation Research, Assistant professor, 大学院・医学系研究科, 助手 (20302672)
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| Project Period (FY) |
2002 – 2003
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| Keywords | SAPK / JNK / wortmannin / hyperthermia / cell death / apoptosis / DNA-PK / Heat sensitization / U937 cells / p53 |
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| Research Abstract |
The overall goal of the present project has been to analyze mechanisms of heat-induced apoptotic cell death and radio-sensitization for better treatment of cancer. Toward this goal, we have accomplished the following progress.
1)We have clarified the involvement of ceramide-SAP/JNK pathway, and radio-sensitization process in heat-induced apoptotic cell death.
2)RT-PCR analysis of apoptosis-related genes revealed that the expression of c-myc gene was reduced in the process of cell death. The introduction of c-myc antisense oligonucleotides or the treatment of c-Myc inhibitor induced cell death.
3)Involvement and the role of DNA-PK in radio-sensitization by heat was evaluated using various repair deficient and knockout mutants of mammalian cells and DT40 chicken cells.
4)We found appearance of fragment of JNK, which were identified as cleaved products by Caspase 3 from INK.
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[Publications] Enomoto, A., Suzuki, N., Kang, Y., Hirano, K., Matsumoto, Y., Zhu, J., Morita, A., Hosoi, Y., Sakai, K., Koyama, H.: "Decreased c-Myc expression and its involvement in X-ray-induced apoptotic cell death of human T-cell leukemia cell line MOLT-4."Int.J.Radiat.Biol.. 79. 589-600 (2003)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Tomita, M., Suzuki, N., Matsumoto, Y., Enomoto, A., Yin, H.-L., Hosoi, Y., Hirano, K., Sakai, K.: "Wortmannin-enhanced X-ray-induced apoptosis of human T-cell leukemia MOLT-A cells possibly through the JNK/SAPK pathway."Radiat.Res.. 160. 467-477 (2003)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Enomoto, A., Suzuki, N., Morita, A., Ito, M., Liu, C.Q., Matsumoto, Y., Yoshioka, K., Shiba, T., Hosoi, Y.: "Caspase-mediated cleavage of INK during stress-induced apoptosis."Biochem.Biophys.Res.Commun.. 306. 837-842 (2003)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Umeda, N., Matsumoto, Y.^*, Yin, H.-L., Tomita, M., Enomoto, A., Morita, A., Mizukoshi, T., Sakai, K., Hosoi, Y., Suzuki, N.: "Difference in the heat sensitivity of DNA-dependent protein kinase activity among mouse, hamster and human cells."Int.J.Radiat.Biol.. 79. 671-680 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Komuro, Y., Watanabe, T., Hosoi, Y., Matsumoto, Y, Nakagawa, K., Saito, S., Ishihara, S., Kazama, S., Tsuno, N., Kitayama, J., Suzuki, N., Tsurita, G., Muto, T., Nagawa, H.: "Prediction of tumor radiosensitivity in rectal carcinoma based on p53 and Ku7O expression."J.ExClin.Cancer Res.. 22. 223-229 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Hosoi, Y., Matsurnoto, Y., Tomita, M., Enomoto, A., Morita, A., Sakai, K., Nakagawa, K., Umeda, N., Zhao, H, -J., Ono, T., Suzuki, N.: "Phosphorothioate oligonucleotides, suramin and heparin inhibit DNA-dependent protein kinase activity."Brit.J.Cancer. 86. 1143-1149 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yoshida, M., Hosoi, Y., Miyachi, H., Ishli, N., Matusmoto, Y., Enomoto, A., Nakagawa, K., Yamada, S., Suzuki, N., Ono, T.: "Roles of DNA-dependent protein kinase and ATM in cell-cycle dependent radiation sensitivity in human cells."Int.J.Radiat.Biol.. 78. 503-512 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Komuro, Y., Watanabe, T., Hosoi, Y., Matsumoto, Y., Nakagawa, K., Kitayama, J., Suzuki, N., Nagawa, H.: "The expression pattern of Ku correlates with tumor radiosensitivity and disease free survival in patients with rectal carcinoma."Cancer. 95. 1195-1205 (2002)
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「研究成果報告書概要(欧文)」より
