2003 Fiscal Year Final Research Report Summary
Analysis of the function of prostasin in the transgenic mice.
| Project/Area Number |
14370321
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | Kumamoto University Graduate School |
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Principal Investigator |
TOMITA Kimio Kumamoto University Graduate School, Nephrology, Professor, 大学院・医学薬学研究部, 教授 (40114772)
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| Co-Investigator(Kenkyū-buntansha) |
KITAMURA Kenichiro Kumamoto University Graduate School, Nephrology, Assistant Professor, 大学院・医学薬学研究部, 助手 (10304990)
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| Project Period (FY) |
2002 – 2003
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| Keywords | Na channel / hypertension / kidney / serine protease / collecting tubule |
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| Research Abstract |
Sodium regulation in the kidney is one of the most important factors for the regulation of blood pressure. Epithelial sodium channel is key protein in the regulation of Na balance. We reported that a new serine protease prostasin can activate epithelial sodium channel in cultured cells. Prostasin was stimulated by aldosterone in vitro and in vivo experiments. In human, urinary prostasin excretion was elevated in primary aldosteronism, suggesting that prostasin may have significant roles in the regulation of blood pressure. Therefore, we have investigated to elucidate the roles of prostasin in the regulation of blood pressure by use of transgenic mice harboring prostasin gene. At first, we have isolated human prostasin cDNA. We have already isolated 3 BAC clones which contained human prostasin gene. This projects is now underway. We must continue further experiments.
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[Publications] Narikiyo T., K.Kitamura, M.Adachi, T.Miyoshi, K.Iwashita, N.Shiraishi, H.Nonoguchi, L.M.Chen, K.X.Chai, J.Chao, K.Tomita: "Regulation of prostasin by aldosterone in the kidney."J.Clin.Invest.. 109. 401-408 (2002)
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[Publications] Iwashita, K., Kitamura, K., Narikiyo, T., Adachi, M., Shiraishi, N., Miyoshi, T., Nagano, J., Tuyen, D.G., Nonoguchi, H., Tomita, K.: "Inhibition of prostasin secretion by serine protease inhibitors in the kidney."J.Am.Soc.Nephrol.. 14. 11-16 (2002)
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[Publications] Shiraishi, N., Kitamura, K., Kohda, Y., Narikiyo, T., Adachi, M., Miyoshi, T., Iwashita, K., Nonoguchi, H., Miller, R.T., Tomita, K.: "Increased endothelin-1 expression in the kidney in hypercalcemic rats."Kidney Int.. 63. 845-852 (2002)
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[Publications] Nonoguchi H, S Kiyama, H Inoue, Y Nakayama, T Inoue, Y Kohda, K Machida, A Tajima, K Kitamura, T Miyoshi, H Shimada, H Shimada, M Tajiri, Y Honda, M Tanaka, K Tomita: "Angiotensin-converting enzyme inhibitor withdrawal and ACE gene polymorphism."Clin Nephrol. 60. 225-232 (2003)
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