2003 Fiscal Year Final Research Report Summary
Analysis on the genes producing the diversity of the cancers, resulting the difficulties of their treatment.
| Project/Area Number |
14370358
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | Kyushu University |
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Principal Investigator |
INOUE Hiroshi Kyushu Unlv., Medical Institute of Bioregulation, Associate Professor, 生体防御医学研究所, 助教授 (90203249)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Masaki Kyushu Unlv., Medical Institute of Bioregulation, Professor, 生体防御医学研究所, 教授 (70190999)
MIMORI Koshi Kyushu Unlv., Medical Institute of Bioregulation, Research Assoctate, 生体防御医学研究所, 助手 (50322748)
UTSUNOMIYA Tohru Kyushu Unfv.Hospital, lecturer, 大学病院, 講師 (30304801)
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| Project Period (FY) |
2002 – 2003
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| Keywords | laser mierodissection / cDNA microarray / T7 amplificationinase / matrix metalloproteinase / lymph node metastasis |
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| Research Abstract |
Purpose : To estimate gastric cancer patients' prognosis more comprehensively, we tried to develop a prognostic scoring system using cDNA microarray.
Experimental Design : RNA was extracted from tumor/normal paired samples of 43 patients with gastric cancer, and cDNA microarray hybridization was performed.
Results : We selected 78 genes that were differentially expressed between aggressive and non-aggressive groups with respect to five conventional pathologic factors. Next, we determined a coefficient for each gene. Thereafter a prognostic score was calculated by summing-up the value for each gene. It ranged from-47 to 201 with a median of 114. There were two peaks in its distribution. Ten of 11 patients who were alive with no evidence of recurrence more than 5 years after the operation showed a score of less than 100 point, while all 19 patients who died of disease showed more than 100 point. In 13 patients who were alive but the follow-uptime was less than 5 years, two of the3 patients with more than 100 point revealed recurrent disease during the follow up.
Conclusions : These findings demonstrate that such a system with cDNA microarray can contribute to the comprehensive analysis of malignant behavior of the tumor and may provide accurate information on prognosis.
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