2005 Fiscal Year Final Research Report Summary
RESEARCH FOR CELL TRANSPLANTATION THERAPY FOR CEREBRAL INFARCTION AND SPINAL CORD INJURY USING BONE MARROW STROMAL CELLS (BMSC)
| Project/Area Number |
14370424
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
KURODA Satoshi HOKKAIDO UNIV., HOKKAIDO UNIVERSITY HOSPITAL, NEUROSURGERY, LEC., 病院, 講師 (10301904)
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| Co-Investigator(Kenkyū-buntansha) |
IWASAKI Yoshinobu HOKKAIDO UNIV., GRADUATE SCHOOL OF MED NEUROSURGERY, PROF., 大学院・医学研究科, 教授 (00113522)
HIDA Kazutoshi HOKKAIDO UNIV., GRADUATE SCHOOL OF MED NEUROSURGERY, ASSIST.PROF., 大学院・医学研究科, 助教授 (10238305)
TAMURA Mamoru HOKKAIDO UNIV., INSTITUTE FOR ELECTRIC SCIENCE, PROF., 電子科学研究所, 教授 (80089888)
TADA Mitsuhiro HOKKAIDO UNIV., INSTITUTE FOR GENETIC MEDICINE, ASSIST.PROF., 遺伝子病制御研究所, 助教授 (10241316)
SHICHINOHE Hideo HOKKAIDO UNIV., HOKKAIDO UNIVERSITY HOSPITAL, POST-DOCTORAL STAFF, 医員 (80374479)
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| Project Period (FY) |
2002 – 2005
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| Keywords | Cerebral infarction / Spinal cord injury / Bone marrow stromal cells / Transplantation / Differentiation / Migration / Biomaterial / Neuroprotection |
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| Research Abstract |
We have established the culture system of the bone marrow stromal cells (BMSC) obtained from the rats and mice, and transplanted them into the animals subjected to cerebral infarction or spinal cord injury. These data were published in Neuropathology (2003) and Brain Res Protoc (2004). We isolated the BMSC from the green fluorescence protein (GFP)-transgenic mice and transplanted them into the mice subjected to cerebral infarction or spinal cord injury. We could visualize the transplanted cells through the skull or the dura mater in the living animals, using a novel fluorescence imaging technique. These data were published in Brain Res Protoc (2004) and J Neurotrauma (2005). Furthermore, we have confirmed that the transplanted BMSC could improve the GABA receptor function around cerebral infarction and spinal cord injury, using autoradiography and fluorescence immunostaining. These data were published in J Nucl Med (2006) and J Neurotrauma (in press). We also found that the BMSC actively repeat cell proliferation when transplanted into infarct brain, and published the data in Brain Res (2005). In addition, we assessed gene expression profile in the cultured BMSC and clarified that the BMSC have the potential to alter their gene expression profile in response to external stimuli. The data were published in Brain Res (2006). We have clarified that the BMSC have the potential to degrade the extracellular matrix in the spinal cord and produce the chemo-attractant factor for axon elongation of the neurons, using organotypic spinal cord slice culture and a novel "in vitro transplantation" model. The data have been submitted to J Neurosurg. We have also found that fibrin glue is a suitable biomaterial as a scaffold for the BMSC when transplanted into brain cold injury model or spinal cord hemi-section model of the rats. These data has been prepared for submission to Neurosurgery and J Neurotrauma.
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