2004 Fiscal Year Final Research Report Summary
Transplantation of monkey ES cell-derived dopaminergic neurons into the brain of MPTP-treated monkey
| Project/Area Number |
14370431
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Kyoto University |
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Principal Investigator |
TAKAHASHI Jun Kyoto University, Graduate School of Medicine, Lecturer, 医学研究科, 講師 (10270779)
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| Co-Investigator(Kenkyū-buntansha) |
NAKATSUJI Norio Kyoto University, Institute for Frontier Medical Sciences, Professor, 再生医科学研究所, 教授 (80237312)
SASAI Yoshiki RIKEN, Center for Developmental Biology, Group Director, 理化学研究所・CDB, グループディレクター (20283616)
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| Project Period (FY) |
2002 – 2004
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| Keywords | ES cell / Cell Transplantation / Parkinson's disease / Dopaminergic neuron |
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| Research Abstract |
Parkinson's disease is a neurodegenerative disorder characterized by loss of midbrain dopaminergic (DA) neurons. ES cells are currently the most promising donor cell source for cell-replacement therapy of Parkinson's disease. We previously described a strong neuralizing activity present on the surface of stromal cells, named SDIA (stromal cell-derived inducing activity). In this study, we generated neurospheres composed of neural progenitors from monkey ES cells, which are capable of producing large numbers of DA neurons. We demonstrated that FGF20, preferentially expressed in the substantia nigra, acted synergistically with FGF2 to increase the number of DA neurons in ES cell-derived neurospheres. We also analyzed the effect of transplantation of DA neurons generated from monkey ES cells into 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-treated monkeys, a primate model for Parkinson's disease. Behavioral studies and functional imaging revealed that the transplanted cells functioned as DA neurons and attenuated MPTP-induced neurological symptoms.
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[Journal Article] Dopaminergic neurons generated from monkey embryonic stem cells function in a Parkinson primate model.2005
Author(s)
Takagi Y, Takahashi J, Saiki H, Morizane A, Hayashi T, Kishi Y, Hukuda H, Okamoto Y, Koyanagi M, Ideguchi M, Hayashi H, Imazato T, Kawasaki H, Suemori H, Omachi S, Iida H, Itoh N, Nakatsuji N, Sasai Y, Hashimoto N.
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Journal Title
J Clin Invest. 115
Pages: 102-109
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Neural precursor cells derived from human embryonic brain retain regional specificity.2004
Author(s)
Horiguchi S, Takahashi J, Kishi Y, Morizane A, Okamoto Y, Koyanagi M, Tsuji M, Tashiro K, Honjo T, Fujii S, Hashimoto N.
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Journal Title
J Neurosci Res. 75(6)
Pages: 817-824
Description
「研究成果報告書概要(欧文)」より
