2003 Fiscal Year Final Research Report Summary
Angiogenesis with bone marrow cells and gene therapy
| Project/Area Number |
14370448
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Osaka Medical College |
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Principal Investigator |
MIYATAKE Shin-ichi Osaka Medical College, Faculty of medicine, Associate Professor, 医学部, 助教授 (90209916)
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| Co-Investigator(Kenkyū-buntansha) |
KUROIWA Toshihiko Osaka Medical College, Faculty of medicine, Professor, 医学部, 教授 (30178115)
KAJIMOTO Yoshinaga Osaka Medical College, Faculty of medicine, Associate Professor, 医学部, 講師 (30224413)
DEGUCHI Jun Osaka Medical College, Faculty of medicine, Associate Professor, 医学部, 講師 (00288713)
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| Project Period (FY) |
2002 – 2003
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| Keywords | bone marrow cell / cerebral infarction / FGF-2 / gene therapy / herpes simplex virus / neurogenesis / transplantation / vasculogenesis |
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| Research Abstract |
To develop the new therapeutic modality for occlusive cerebrovascular disease, we try to combine the cell therapy using bone marrow cells and gene therapy using viral vectors. We performed next 3 projects.
1)Direct gene therapy using FGF-2-expressing adenoviral vectors into lateral ventricle in MCA occlusiojn rat.
2)Development of new, replication incompetent herpes simplex virus (HSV) vector expressing FGF-2, VEGF and HGF. Also new attempt to transfer the above gene into bone marrow cells using this new vectors.
3)Therapeutic experiment using mesenchymal stromal cells (MSC) and this new developed HSV vectors.
Results :
1)Direct gene delivery of FGF-2 decreased the infarction size and improved the neurological symptom after MCA occlusion.
2)Newly constructed HSV showed marked gene transduction efficacy into mesenchymal stromal cells and exhibited stable and strong gene expression in the MSC. No viral replication in MSC was recognized.
3)Intralesional transplantation of MSC improved the neurological symptom in MCA occlusion rat, while, that of MSC transduced HGF gene by HSV vector exhibited more improvement of the symptom in model rat.
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