2003 Fiscal Year Final Research Report Summary
Is stem cell present in human endometrium?
| Project/Area Number |
14370522
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tohoku University |
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Principal Investigator |
MURAKAMI Takashi Tohoku University, Graduate School of Medicine, Associate Professor, 大学院・医学系研究科, 助教授 (20240666)
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| Co-Investigator(Kenkyū-buntansha) |
TERADA Yukihiro Tohoku University, Hospital, Associate Professor, 病院・助教授 (10260431)
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| Project Period (FY) |
2002 – 2003
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| Keywords | endometrium / endometriosis / clonality |
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| Research Abstract |
Objective : To investigate the clonality of ectopic gland cells in peritoneal endometriosis
Design : Prospective study
Setting : University hospital
Patient(s) : Seven-teen women with surgically diagnosed endometriosis.
Intervention(s) : Samples of peritoneal endometriotic lesions were obtained from patients during laparoscopic surgery.
Main Outcome Measure(s) : Clonality analysis using the laser microdissection technique, a phosphoglycerate kinase (PGK) gene polymorphism assay, and an androgen receptor (AR) gene polymorphism assay following digestion of the DNA with methylation-sensitiveendonuclease were used.
Result(s) : Each ectopic gland of the peritoneal endometriotic lesion showed a monoclonal pattern in both the PGK gene and AR gene assays, but the methylation pattern of the PGK gene and/or AR gene was divergent among adjacent glands in the lesion. These data indicate that the peritoneal endometriotic lesions are multicellular in origin, although individual glands of the lesion are derived from single precursor cells.
Conclusion(s) : The colored peritoneal endometriotic lesion in the present study was multicellular in origin. Peritoneal endometriotic lesions may thus be initiated by transplantation of a cluster of eutopic endometrial tissues into the pelvis.
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