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2004 Fiscal Year Final Research Report Summary

The mechanism in the diversity and amplification of mucosal immune system -Clinical application of mucosal vaccine-

Research Project

Project/Area Number 14370548
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Otorhinolaryngology
Research InstitutionKagoshima University

Principal Investigator

KURONO Yuichi  Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (80153427)

Co-Investigator(Kenkyū-buntansha) MATSUNE Shoji  Kagoshima University, Graduate School of Medical and Dental Sciences, Associate Professor, 大学院・医歯学総合研究科, 助教授 (00253899)
USHIKAI Masato  Kagoshima University, University Hospital, Faculty of Medicine and Dentistry, Assistant Professor, 医学部・歯学部附属病院, 講師 (00284886)
NISHIMOTO Kengo  Kagoshima University, University Hospital, Faculty of Medicine and Dentistry, Research Associate, 医学部・歯学部附属病院, 助手 (50305132)
FUKUIWA Tatsuya  Kagoshima University, Graduate School of Medical and Dental Sciences, Research Associate, 大学院・医歯学総合研究科, 助手 (60325785)
AKASHI Mitsuru  Osaka University, Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (20145460)
Project Period (FY) 2002 – 2004
KeywordsHaemophilus influenzae / Streptococcus pneumoniae / Phosphorylcholine / Mucosal vaccine / Intra-nasal vaccination / IgA / IgE / Type I allergy
Research Abstract

1.Immune responses against Haemophilus influenzae(Hi) P6.
Recombinant P6(rP6) and nanosphere were coupled and intra-nasally administered to mice. Although P6-specific serum IgM and IgG levels were increased, secretory IgA was not induced in external secretions, indicating the low antigenesity of rP6 as mucosal antigen.
2.Mucosal immune responses against phosphorylcholine(PC).
PC is contained in all Gram-positive and -negative bacteria. However, the antigenesity of PC is not yet clarified. When PC was intra-nasally administered to mice, systemic as well as mucosal immune responses were induced without using any mucosal adjuvant such as cholera toxin. Moreover, the antibodies induced by PC were cross-reactive with several different strains of Hi and Streptococcus pneumoniae. Those results suggest that PC might be a candidate of mucosal vaccine with broad spectrum.
3.Inhibition of type I allergy by immunization with PC.
Mucosal vaccine frequently induces type I allergy by producing IgE. However, when PC was intra-nasally or intra-peritoneally administered to mice, the production of IgE was significantly reduced. Further, splenic helper T cells isolated from immunized mice with PC produced much less IL-4 compared with the controls. Those findings suggest the efficacy and the safety of PC as a promising mucosal vaccine.

  • Research Products

    (14 results)

All 2004 2003 2002

All Journal Article (14 results)

  • [Journal Article] The role of allergic rhinitis in upper respiratory tract inflammatory diseases.2004

    • Author(s)
      Y.Kurono, S.Matsune et al.
    • Journal Title

      Clin Exp All Rev 4

      Pages: 15-20

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 上気道粘膜免疫の誘導機序2004

    • Author(s)
      黒野祐一
    • Journal Title

      耳鼻免疫アレルギー 22(1)

      Pages: 1-5

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Cytokine expression in experimental chronic otitis media with effusion in mice.2004

    • Author(s)
      Kazuhiko Maeda, Yuichi Kurono, et al.
    • Journal Title

      Laryngoscope 114(11)

      Pages: 1967-1972

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The role of allergic rhinitis in upper respiratory tract inflammatory diseases.2004

    • Author(s)
      Yuichi Kurono, Shoji Matsune, Dong Sun, Masashi Suzuki, Goro Mogi
    • Journal Title

      Clin Exp All Rev 4

      Pages: 15-20

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The mechanism in inducing upper respiratory mucosal immune system.2004

    • Author(s)
      Y.Kurono
    • Journal Title

      JJIAO 22(1)

      Pages: 1-5

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cytokine expression in experimental chronic otitis media with effusion in mice.2004

    • Author(s)
      Kazuhiko Maeda, Takashi Hirano, Issei Ichimiya, Yuichi Kurono, Masashi Suzuki, Goro Mogi
    • Journal Title

      Laryngoscope 114(11)

      Pages: 1967-1972

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] NALTの形成と機能発現におけるLTとNIKの役割2003

    • Author(s)
      福山 聡, 黒野祐一 他
    • Journal Title

      耳鼻免疫アレルギー 21(1)

      Pages: 15-18

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] 上気道の生体防御機構2003

    • Author(s)
      福山 聡, 黒野祐一
    • Journal Title

      JHONS 19(6)

      Pages: 779-782

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The role of nuclear factor-KB in interleukin-8 expression by human adenoidal fibroblasts.2003

    • Author(s)
      Takaki M., Kurono Y.et al.
    • Journal Title

      Laryngoscope 113(8)

      Pages: 1378-1385

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] The role of Lymphotoxin and NF-κB inducing kinase for the development and the function of nasopapharymgeal-associated lymphoid tissue2003

    • Author(s)
      Satoshi Fukuyama, Takashi Shikina, Yuichi Kurono, Hiroshi Kiyono
    • Journal Title

      JJIAO 21(1)

      Pages: 15-18

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mucosal immune system of upper respiratory tract.2003

    • Author(s)
      S.Fukuyama, Y.Kurono
    • Journal Title

      JOHNS 19(6)

      Pages: 779-782

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The role of nuclear factor-κB in interleukin-8 expression by human adenoidal fibroblasts.2003

    • Author(s)
      Takaki M, Ushikai M, Deguchi K, Nishimoto K, Matsune S, Kurono Y.
    • Journal Title

      Laryngoscope 13(8)

      Pages: 1378-1385

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Initiation of NALT organogenesis is independent of the IL-7R, LTβR, and NIK signaling pathways but requires the Id2 gene and CD3^- CD4^+ CD45^+ cells.2002

    • Author(s)
      Fukuyama S., Kurono Y.et al.
    • Journal Title

      Immunity 17(1)

      Pages: 31-40

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Initiation of NALT organogenesis is independent of the IL-7R, LTβR, and NIK signaling pathways but requires the Id2 gene and CD3^- CD4^+ CD45^+ cells.2002

    • Author(s)
      Fukuyama S, Hiroi T, Yokota Y, Rennert PD, Yanagita M, Kinoshita N, Terawaki S, Shikina T, Yamamoto M, Kurono Y, Kiyono H
    • Journal Title

      Immunity 17(1)

      Pages: 1-40

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2006-07-11  

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