• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2005 Fiscal Year Final Research Report Summary

Molecular mechanisms of the response to low-dose radiation in hTERT-immortalized human cells

Research Project

Project/Area Number 14380260
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 環境影響評価(含放射線生物学)
Research InstitutionAichi Cancer Center

Principal Investigator

ISHIZAKI Kanji  Aichi Cancer Center, Radiation Biol., Head of Lab., 中央実験部, 部長 (70111987)

Co-Investigator(Kenkyū-buntansha) KUMIMOTO Hiroshi  Aichi Cancer Center, Radiation Biology, Researcher, 中央実験部, 研究員 (00291170)
Project Period (FY) 2002 – 2005
KeywordshTERT gene / human cells / low-dose, low-dose-rate radiation / DNA repair / ATM protein / damage response
Research Abstract

In this study, we used human cells immortalized by introduction of the hTERT gene to reveal the effects of low-dose and low-dose-rate radiation on cells in human bodies. Cells derived from normal individuals were very resistant to low-dose-rate radiation since DNA damage induced by low-dose-rate radiation was efficiently repaired in these cells. During irradiation at low-dose-rate, ATM protein was partly activated but downstream p53 protein was barely activated. On the contrary, AT cells defective in ATM protein were sensitive to low-dose-rate radiation as like as to high-dose-rate radiation. We also found that some part of DNA damage induce by low-dose-rate irradiation in AT cells were not repaired for long time. These results suggest that ATM protein partly activated in cells irradiated with low-dose or at low-dose-rate can promote repair of DNA damage induced by these radiation but can not induce downstream checkpoint function.
To search change of expression of various genes during low-dose-rate irradiation, we performed micro-array analysis using 25k cDNA arrays. Among variety of genes those showed change of expression, we interested in the histone H1 family genes. It is known that histone proteins belonging to H2 and H3 families are involved in chromatin reconstruction but in our experiments expression of the genes of these proteins were not changed during low-dose-rate irradiation. Using real-time PCR, we further analyzed expression of these histone H1 family genes and confired increased expression during low-dose-rate radiation. We are now analyzing function of histon H1 family protein in DNA damage response.

  • Research Products

    (10 results)

All 2005 2004 2003 2002 Other

All Journal Article (10 results)

  • [Journal Article] Cytotoxic and Mutagenic Effects of Chronic Low-Dose-Rate Irradiation on hTERT-Immortalized Human Cells.2005

    • Author(s)
      Nakamura, H. et al.
    • Journal Title

      Radiation Research 163

      Pages: 283-288

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Activation of ATM DNA damage checkpoint signal transduction elicited by Herpes simplesx virus infection.2005

    • Author(s)
      Shirata, N. et al.
    • Journal Title

      J. Biol. Chemist. 280

      Pages: 30336-30341

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Activation of ATM DNA damage checkpoint signal transduction elicited by Herpes simplex virus infection.2005

    • Author(s)
      Shirata, N. et al.
    • Journal Title

      J.Biol.Chemist. 280

      Pages: 30336-30341

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] No induction of p53 phosphorylation and few focus formation of γ H2AX suggest efficient repair of DNA damage by LDR irradiation.2004

    • Author(s)
      Ishizak, K. et al.
    • Journal Title

      J. Radiation Res. 45

      Pages: 521-525

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] No induction of p53 phosphorylation and few focus formation of γ H2AX suggest efficient repair of DNA damage by LDR irradiation.2004

    • Author(s)
      Ishizaki, K. et al.
    • Journal Title

      J.Radiation Res. 45

      Pages: 521-525

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] テロメラーゼ遺伝子導入によるヒト細胞の不死化2003

    • Author(s)
      中村英亮 他
    • Journal Title

      放射線生物研究 38

      Pages: 43-52

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Establishment of immortal normal and ataxia telangiectasia fibroblast cell lines by introduction of the hTERT gene.2002

    • Author(s)
      Nakamura, H et al.
    • Journal Title

      J. Radiation Res. 43

      Pages: 167-174

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Establishment of immortal normal and ataxia telangiectasia fibroblast cell lines by introduction of the hTERT gene.2002

    • Author(s)
      Nakamura, H et al.
    • Journal Title

      J.Radiation Res. 43

      Pages: 167-174

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] DNA repair defect in AT cells and their hypersensitivity to low-dose-rate radiation.

    • Author(s)
      Nakamura, H. et al.
    • Journal Title

      Radiation Research (印刷中)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] DNA repair defect in AT cells and their hypersensitivity to low-dose-rate radiation.

    • Author(s)
      Nakamura, H. et al.
    • Journal Title

      Radiation Research (in press)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2007-12-13  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi