• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2003 Fiscal Year Final Research Report Summary

The role of calcium-mobilizing signal transduction system in fertilization

Research Project

Project/Area Number 14380302
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Functional biochemistry
Research InstitutionTokyo University of Pharmacy and Life Science (2003)
The University of Tokyo (2002)

Principal Investigator

FUKAMI Kiyoko  Tokyo University of Pharmacy and Life Science, School of Life Science, Professor, 生命科学部, 教授 (40181242)

Project Period (FY) 2002 – 2003
Keywordsfertilization / calcium / Phosphatidyl inositol turnover / Phospholipase C / acrosome reaction
Research Abstract

Zona-Pellucida(ZP)-induced acrosome reaction in sperm is a required step for mammalian fertilization. However, the precise mechanism of the acrosome reaction remains unclear. We previously reported that Phospholipase C delta 4 (PLCδ4) is involved in ZP-induced acrosome reaction in mouse sperm. Here we have monitored Ca^<2+> responses in single sperm, and we report that the [Ca^<2+>]_i increase in response to ZP, which is essential for driving the acrosome reaction in vivo, is absent in PLCδ4^<-/-> sperm. Progesterone, another physiological inducer of the acrosome reaction, failed to induce sustained [Ca^<2+>]_i increases in PLCδ4^<-/-> sperm and consequently the acrosome reaction was partially inhibited. In addition, we observed oscillative [Ca^<2+>]_i increases in wild-type sperm in response to these acrosome inducers. Calcium imaging studies revealed that the [Ca^<2+>]_i increases induced by exposure to ZP and progesterone started at different sites within the sperm head, indicating that these agonists induce the acrosome reaction via different Ca^<2+> mechanisms. Furthermore, store-operated channel (SOC) activity was severely impaired in PLCδ4^<-/-> sperm. These results indicate that PLCδ4 is an important enzyme for intracellular [Ca^<2+>]_i mobilization in ZP-induced acrosome reaction and for sustained [Ca^<2+>]_i increases through SOC induced by ZP and progesterone in sperm.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Fukami, K., et al.: "Phospholipase Cδ4 is required for [Ca^<2+>]_i mobilization essential for acrosome reaction in sperm."J.Cell Biol.. 161. 79-88 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakamura, Y., et al.: "Phospholipase Cδ1 is required for skin stem cell lineage commitment"EMBO J.. 22. 2981-2991 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kei Takenaka, et al.: "The role of Phospholipase C-L2, a novel phospholipase C like protein that lacks lipase activity, in B cell receptor signaling."Mol.Cell.Biol.. 20. 7329-7338 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zen Kouchi, et al.: "Recombinant Phospholipase C-zeta has high Ca^<2+>-sensitivity and induces Ca^<2+> oscillations in mouse eggs."J.Biol.Chem.. 279. 10408-10412 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kurokawa, M et al.: "Evidence that activation of a src family kinase is not required for fertilization-associated [Ca^<2+>] oscillations in mouse eggs."Reproduction. 127. 441-454 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukami, K., et al.: "Phospholipase Cδ4 is required for [Ca^<2+>]_i mobilization essential for acrosome reaction in sperm."J.Cell Biol.. 161. 79-88 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakamura, Y., et al.: "Phospholipase Cδ1 is required for skin stem cell lineage commitment."EMBO J.. 22. 2981-2991 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takenaka, K.et al.: "The role of Phospholipase C-L2, a novel phospholipase C like protein that lacks lipase activity, in B cell receptor signaling."Mol.Cell.Biol.. 20. 7329-7338 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kouchi, Z.et al.: "Recombinant Phospholipase C-zeta has high Ca^<2+>-sensitivity and induces Ca^<2+> oscillations in mouse eggs."J.Biol.Chem.. 279. 10408-10412 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kurokawa, M., et al.: "Evidence that activation of a src family kinase is not required for fertilization-associated [Ca^<2+>] oscillations in mouse eggs."Reproduction. 127. 441-454 (2004)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2005-04-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi