2004 Fiscal Year Final Research Report Summary
Elucidation of regulatory mechanisms coordinating forespore membrane assembly with meiotic nuclear divisions
| Project/Area Number |
14380338
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Graduate School of Science, Osaka City University |
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Principal Investigator |
SHIMODA Chikashi Osaka City University, Department of Biology, Graduate School of Science, Professor, 大学院・理学研究科, 教授 (80047290)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Taro Osaka City University, Department of Biology, Graduate School of Science, Lecturer, 大学院・理学研究科, 講師 (30291082)
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| Project Period (FY) |
2002 – 2004
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| Keywords | sporulation / meiosis / fission yeast / mitotic apparatus / spindle / spincle pole body (SPB) / checkpoint |
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| Research Abstract |
The forespore membrane is formed during second meiotic division in fission yeast. The coordination of the forespore membrane assembly with the progression of meiotic divisions is crucial for accurate transmission of the whole genome to spores.
1. In order to elucidate this coupling mechanism, the mutants of S pombe that are defective in repression of the forespore membrane assembly in meiosis I. We isolated and analyzed gis mutants that forms preferentially two-spoted asci. These gis mutants initiate the forespore membrane at meiosis L The gis genes have been identified as β-tubulin, Alp7 and Alp14, all of which are involved in spindle formation or spindle/kinetochore attachment. Treatment of wild-type cells with the microtubule-depolymerizing drug, TBZ, induced two-spored asci like in gis mutants.
2. To examine whether spindle checkpoint is activated in the gis mutants, the mad2 mutation was introduced into the respective gis mutants. Most of these double mutants are lethal. The gis1 mad2 double mutants, which are defective in spindle assembly checkpoint, partially suppressed the phenotypes of the gist single mutants, thus form normal four-spored ascus. In the gis1 mad2 double mutants, the duration of the metaphase I is considerably extended, suggesting activation of the spindle assembly checkpoint TBZ-induced formation of two-spored asci is scarcely observed in mad2 mutants.
3. These results support the idea that the delay of the first meiotic division is responsible for the precocious formation of the forespore membrane at meiosis I.
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[Journal Article] Characterization of O-mannosyltransferase family in Schizosaccharomyces pombe.2005
Author(s)
Tanaka, N., Fujita, Suzuki, S., Morishita, M., Giga-Hama, Y., Shimoda, C., Takegawa, K.
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Journal Title
Biochem. Biophys. Commun. 330
Pages: 813-820
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Characterization of O-mannosyltransferase family in Schizosaccharomyces pombe.2005
Author(s)
Tanaka, N., Fujita, Y., Suzuki, S., Morishita, M., Giga-Hama, Y., Shimoda, C., Takegawa, K.
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Journal Title
Biochem.Biophys.Res.Commun. 330
Pages: 813-820
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Sorting nexin homologues are targets of phosphatidylinositol 3-phospate in sporulation of Schizosaccharomyces pombe.2004
Author(s)
Koga, T., Onishi, M., Nakamura, Y., Hirata, A., Nakamura, T., Shimoda, C.et al.
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Journal Title
Genes Cells 9
Pages: 561-574
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Sorting nexin homologues are targets of phosphatidylinositol 3-phosphate in sporulation of Schizosaccharomyces pombe.2004
Author(s)
Koga, T.Onishi, M., Nakamura, Y., Hirata, A., Nakamura, T., Shimoda, C., Iwai, T., Takegawa, K., Fukui, Y.
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Journal Title
Genes Cells 9
Pages: 561-574
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Role of phosphatidylinositol 3-phosphate in formation of forespore membrane in schizosaccharomyces pombe.2003
Author(s)
Onishi, M., Koga, T., Norita, R., Nakamura, Y., Nakamura, T., Shimoda, C., Takegawa, K., Hirata, A., Fukui, Y.
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Journal Title
Description
「研究成果報告書概要(和文)」より
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[Journal Article] The Schizosaccharomyces pombe cdt2^+ gene, a target of G1-S phase-specific transcription factor complex DSC1, is required for mitotic and premeiotic DNA replication.2003
Author(s)
Yoshida, S., Al-Amodi, H., Nakumura, T., McInerny, C.J., Shimoda, C.
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Journal Title
Genetics 164
Pages: 881-893
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Role of phosphatidylinositol 3-phosphate in formation of forespore membrane in Schizosaccharomyces pombe.2003
Author(s)
Onishi, M., Koga, T., Morita, R., Nakamura, Y., Nakamura, T., Shimoda, C., Takegawa, K., Hirata, A., Fukui, Y.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] The Schizosaccharomyces pombe cdt2^+ gene, a target of G1-S phase-specific transcription factor complex DSC1, is required for mitotic and premeiotic DNA replication.2003
Author(s)
Yoshida, S., Al-Amodi, H., Nakamura, T., McInerny, C.J., Shimoda, C.
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Journal Title
Genetics 164
Pages: 881-893
Description
「研究成果報告書概要(欧文)」より
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