2003 Fiscal Year Final Research Report Summary
The role of Notch signaling in the transdetermination
| Project/Area Number |
14380343
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Tohoku University |
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Principal Investigator |
KURATA Shoichiro Tohoku University, Graduate School of Pharmacoutical Sciences, Associate Professor, 大学院・薬学研究科, 助教授 (90221944)
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| Project Period (FY) |
2002 – 2003
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| Keywords | determination of cell fate / Notch signaling / transdetermination |
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| Research Abstract |
One of the fundamental questions in the developmental biology is how organ identity is determined. We have established a system, using transgenic Drosophila, which allows eye to wing, leg and antenna transformation by the activation of the Notch signaling. To determine the role of the Notch signaling in the transdetermination, we performed the GAL4-mediated gain-of-function mutant screen in the combination with the activation of the Notch signaling, using the GS strains in which a target sequence for GALA called UAS is inserted at random. Up to now about 10,000 strains were screened, and three strains were found to induce the eye to wing transformation. And the eye to antenna and the eye to leg transformation were induced in 23 strains and 11 strains, respectively. A novel gene encoding a Bromo-adjacent homology (BAH) domain protein was identified as a corresponding gene of a strain which induce the eye to wing transformation. The over-expression of the BAH domain protein was found to induce ectopic activation of the wing-specific enhancer of vestigial (vg) gene which regulates wing development, suggesting that the BAH domain protein regulates wing development up-stream of vg. Corresponding to this result, the BAH domain protein induced ectopic wings having antero-posterior and dorso-ventral axis which are not formed in the vg-induced ectopic wings. To confirm that the Notch signaling has similar function in the vertebrate development, we found that the Notch signaling regulates the expression of Pax6 gene in Xenopus eye development as well as Drosophila.
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Research Products
(13 results)
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[Publications] Yajima, M., Takada, M., Takahashi, N., Kikuchi, H., Natori, S., Oshima, Y., Kurata, S: "A newly established in vitro culture using transgenic Drosophila revealed functional coupling between the phospholipase A2-generated fatty acid cascade and lipopoly saccharide-dependent activation of the imd pathway in insect immunity."Biochem.J.. 371. 205-210 (2003)
Description
「研究成果報告書概要(和文)」より
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[Publications] Takehana, A., Katsuyama, T., Yano, T., Oshima, Y., Takada, H., Aigaki, T., Kurata S.: "Overexpression of a pattern recognition receptor, peptidoglycan recognition protein-LE, activates Imd/Reish-mediated antibacterial defense and prophenoloxidase cascade in Drosophila larvae"Proc.Natl.Acad.Sci.USA.. 99. 13705-13710 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Ikejima, M., Nozaki, T., Kurata, S., Natori, S., Esumi, H., Sugimura, T., Masutani, M: "Purification and characterization of poly(ADP-ribose) polymerase cDNA from Sarcophaga peregrina"Proc.Japan Acad.. 78. 282-285 (2002)
Description
「研究成果報告書概要(和文)」より
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[Publications] Punzo, C., Plaza, S., Seimiya, M., Kurata, S., Schnupf, P., Jager, J., Gehring, W.: "Eyeless and twin of eyeless conservations and differences in Drosophila melanogaster"Development. 印刷中.
Description
「研究成果報告書概要(和文)」より
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[Publications] Takehana, A., Katsuyama, T., Yano, T., Oshima, Y., Takada, H., Aigaki, T., Kurata, S.: "Overexpression of a pattern-recognition receptor, peptidoglycan-recognition protein-LE, activates imd/relish-mediated antibacterial defense and the prophenoloxidase cascade in Drosophila larvae"Proc.Natl.Acad.Sci.USA. 99. 13705-13710 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Ikejima, M., Nozaki, T., Kurata, S., Natori, S., Esumi, H., Sugimura, T., Masutani, M.: "Purification and characterization of poly(ADP-ribose) polymerase cDNA from Sarcophaga peregrina"Proc.Japan Acad.. 78. 282-285 (2002)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Yajima, M., Takada, M., Takahashi, N., Kikuchi, H., Natori, S., Oshima, Y., Kurata, S.: "A newly established in vitro culture using transgenic Drosophila reveals functional coupling between the phospholipase A2-generated fatty acid cascade and lipopolysaccharide-dependent activation of the immune deficiency the imd pathway in insect immunity"Biochem.J.. 371. 205-210 (2003)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Punzo, c., Plaza, S., Seimiya, M., Kurata, S., Schnupf, P., Jager, J., Gehring, W.J.: "Eyeless and twin of eyeless conservations and differences in Drosophira melanogaster"Development. (in press).
Description
「研究成果報告書概要(欧文)」より
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