2003 Fiscal Year Final Research Report Summary
Isolation and characterization of a receptor candidate for L-DOPA in C.elegans
| Project/Area Number |
14380360
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurochemistry/Neuropharmacology
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| Research Institution | Yokohama City University |
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Principal Investigator |
GOSHIMA Yoshio Yokohama City Univ., Sch.of Med., Professor, 医学研究科, 教授 (00153750)
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| Co-Investigator(Kenkyū-buntansha) |
KATURA Isao National Institute of Genetics, Professor, 教授 (00107690)
OGURA Ken-ichi Yokohama City University, Sch.of Med., Assistant Professor, 医学部, 助手 (20326028)
NAKAMURA Fumio Yokohama City Univ., Sch.of Med., Assistant Professor, 医学部, 講師 (10262023)
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| Project Period (FY) |
2002 – 2003
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| Keywords | L-DOPA / transmitter / receptor / G-protein / C.elegns / pumping behavior / chemosensory / Xenopus |
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| Research Abstract |
We have identified a candidate receptor for L-DOPA (CeDOPAR) in C.elegans. CeDOPAR share homology with G protein-coupled receptor. In CeDOPAR-expressed Xenopus laevis oocyte, both L-DOPA (pM order) and L-theo-DOPS (mM order) induced inward current. DOPS-induced response was inhibited by DOPA-CHE, an antagonist for L-DOPA, The immunohistochemistry with specific antibody raised against CeDOPAR, suggests that CeDOPAR expression was regulated at different stages. In adult, CeDOPAR was localized near M1 that is thought to control pharyngeal pumping of the worm. On the dopa-containing agar plate, pumping rate of worm was significantly increased when compared that on control agar plate. RNAi for CeDOPAR suppressed the pumping response to DOPA, thereby indicating that CeDOPAR was a candidate receptor mediating the DOPA-induced behavior in nematode. We are now searching various ligands for CeDOPAR.
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