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2004 Fiscal Year Final Research Report Summary

Development of monitaruin system for intestinal flora of experimental animals using FISH method

Research Project

Project/Area Number 14380379
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionThe University of Tokyo

Principal Investigator

ITOH Kikuji  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Associate Professor, 大学院・農学生命科学研究科, 助教授 (50100045)

Co-Investigator(Kenkyū-buntansha) HIRAYAMA Kazuhiro  The University of Tokyo, Graduate School of Agricultural and Life Sciences, Assistant, 大学院・農学生命科学研究科, 助手 (60208858)
SUEMIZU Youji  Central Institute of Laboratory Animals, Researcher, 研究員 (40332209)
Project Period (FY) 2002 – 2004
KeywordsIntestinal flora / FISH method / Fusiform shaped bacteria / Bacteroidaseae / Clostridium / Lactobacillus / 16S rDNA
Research Abstract

We designed four group specific probes for Clostridium based on the 16S rDNA sequenc3es of isolates and directly cloned DNA from mouse feces. For detection of Lactobacillus 12 probes were prepared, Lab722 (Lactobacillus group), Labc (L.acidophilus), Lbam (L.amylovorus), Lber (L.crispatus), Lbga (L.gasseri), Lbin (L.intestinalis), Lbjg (L.johnsonii/L.gasseri), Lbjh (L.johnsonii), Lbmu (L.murinus/L.animalis), Lbpa (L.paracasei), Lbre (L.reuteri), Lbva (L.vaginalis). Lbin, Lbjg, Lbmu, Lbva were designed in this study using 16S and 23S rDNA sequences. For Bacteroides, nine probes for Bacteroides species and two probes for two Bacteroides groups. They were Bac303 for Bacteroides cluster, Bfa602 for Bacteroides group, Baf1001 for B.acidofaeciens, Bdis656 for B.distasonis, Bfra998 for B.fragilis, Bova for B.ovatus, Bth172 for B.thetaiotaomicron, Buni for B.uniformis, Bvul for B.vulgatus. Baf1001 and Bth172 were designed in this study and Bova, Buni and Buni were designed in our previous study. These probes showed high specificity and detected each group in mouse feces using FISH.

  • Research Products

    (12 results)

All 2005 2004 2003 2002

All Journal Article (12 results)

  • [Journal Article] Intestinal Flora of Animal Models of Human Diseases as an Environmental Factor2005

    • Author(s)
      Itoh, K., Narushima, S.
    • Journal Title

      Curr. Issues Intestinal Microbiol. 6

      Pages: 9-16

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Characteristic Intestinal microflora of specific pathogen-free mice bred in two different colonies and their Influence on Postnatal Murine Immunocyte Profiles2005

    • Author(s)
      Nagura, T., Itoh, K.他
    • Journal Title

      Exp.Anim. 54

      Pages: 143-148

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Species-specific oligonucleotide probes for the detection and identification of Lactobachillus isolated from mouse faeces2005

    • Author(s)
      Park, S.H., Itoh, K.
    • Journal Title

      J.Appl.Microbiol. 98(in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Intestinal Flora of Animal Models of Human Diseases as an Environmental Factor Curr.2005

    • Author(s)
      Itoh, K., Narushima, S.
    • Journal Title

      Issues Intestinal Microbiol. 6

      Pages: 9-16

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characteristic Intestinal microflora of specific pathogen-free mice bred in two different colonies and their influence on postnatal murine immunocyte profiles.2005

    • Author(s)
      Nagura, T., Hachimura, S., Kaminogawa, S., Aritsuka, T., Itoh, K.
    • Journal Title

      Exp.Anim. 54(2)

      Pages: 143-148

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Species-specific oligonucleotide probes for the detection and identification of Lactobachillus isolated from mouse faeces.2005

    • Author(s)
      Park, S.H., Itoh, K.
    • Journal Title

      J.Appl.Microbiol. 98 (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Muscularis inflammation and the loss of intestinal cells of Cajal in the endothelin ETB receptor null rat.2004

    • Author(s)
      Suzuki, T., Itoh, K.他
    • Journal Title

      Am.J.Physiol.Gastrintest. Liver Physiol. 287

      Pages: 638-646

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Muscularis inflammation and the loss of intestinal cells of Cajal in the endothelin ETB receptor null rat.2004

    • Author(s)
      Suzuki, T., Won, K.J., Horiguchi, K., Kinoshita, K., Hori, M., Torihashi, S., Momotani, E., Itoh, K., Hirayama, K., Ward, S.M., Sanders, K.M., Ozaki, H.
    • Journal Title

      Am.J.Physiol.Gastrintest.Liver Physiol. 287

      Pages: G638-G646

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Abnormal intestinal environment in rats with spontaneouse osinophilia (MES rat) : A possible new model for Studying Intestinal Putrefaction2003

    • Author(s)
      Hirayama, K., Kouno, J., Itoh, K., Matsumoto, K.
    • Journal Title

      Microbial Ecol. In Health and Dis. 15

      Pages: 57-62

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Abnormal intestinal environment in rats with spontaneous eosinophilia(MES rat) : A possible new model for studying intestinal putrefaction.2003

    • Author(s)
      Hirayama, K., Kouno, J.Itoh, K., Matsumoto, K.
    • Journal Title

      Microbial Ecology in Health and Disease 15

      Pages: 57-62

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Distribution analysis of six predominant Bacteroides species in normal human feces using 16S rDNA-targeted Species-Specific Primers2002

    • Author(s)
      Miyamoto, Y., Watanabe, K., Tanaka, R., Itoh, K.
    • Journal Title

      Microbial Ecology in Health and Disease 14

      Pages: 133-136

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Distribution analysis of six predominant Bacteroides species in normal human feces using 16S rDNA-targeted species-specific primers.2002

    • Author(s)
      Miyamoto, Y., Watanabe, K., Tanaka, R., Itoh, K.
    • Journal Title

      Microbial Ecology in Health and Disease 14

      Pages: 133-136

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2006-07-11  

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