2004 Fiscal Year Final Research Report Summary
Regeneration of cartilage from autologous stem cells employing a loading system of physiological stress
| Project/Area Number |
14380401
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Hokkaido University (2004)
Osaka University (2002-2003) |
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Principal Investigator |
TAKAGI Mutsumi Hokkaido University, Graduate School b of Engineering, Professor, 大学院・工学研究科, 教授 (20263212)
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| Co-Investigator(Kenkyū-buntansha) |
WAKITANI Shigeyuki Shinshu University School of Medicine, Orthopedic Surgery, Lecturer, 医学部, 講師 (70243243)
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| Project Period (FY) |
2002 – 2004
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| Keywords | cartilage / three-dimensional culture / collagen gel / aggrecan / pressurization / culture apparatus / physical loading / biodegradable polymer |
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| Research Abstract |
Loading of physical stress is considered to be important for cartilage tissue, because chondrocyte cells in cartilage in vivo receive cyclic loading of physical stress. The novel cultivation apparatus which can load cells with physical stress having optional amplitude (5-40mm), velocity (0-100spm), cycle (0-1Hz), displacement (0-2,000μm), timing (1min-99h) through vertical move of a piston head, was developed in this study, aiming the utilization of physical stress loading for regeneration of cartilage tissue.
Physical stress with displacement of 0.5mm was loaded dynamically or statically to three-dimensional culture of chondrocyte cells embedded into collagen gel which depth was 2mm. Amplitude, velocity, and timing were maintained to a constant values. The cycle of dynamic stress was adjusted to 0.33Hz, which was the cycle of walking. The dynamic stress resulted in the twice level of glucose consumption rate compared with the rate with static stress. However, the stress did not affect cell density and decrease the expression of aggrecan mRNA.
Lowering the cycle of dynamic stress from 0.33 to 0.01Hz and decrease of loading time of dynamic and static stress could not improve cell density and the mRNA expression.
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