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2004 Fiscal Year Final Research Report Summary

Molecular mechanisms of the role of matrix metalloproteinase derived from mast cells in the process of allergic responses

Research Project

Project/Area Number 14390020
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field 広領域
Research InstitutionNational University Corporation Tokyo University of Agriculture and Technology

Principal Investigator

MATSUDA Hiroshi  National University Corporation Tokyo University of Agriculture and Technology, Graduate School, Institute of Symbiotic Science and Technology, Professor, 大学院・共生科学技術研究部, 教授 (80145820)

Co-Investigator(Kenkyū-buntansha) ARAI Katsuhiko  National University Corporation Tokyo University of Agriculture and Technology, Faculty of Agriculture, Associate Professor, 農学部, 助教授 (60175940)
WATANABE Gen  National University Corporation Tokyo University of Agriculture and Technology, Graduate School, Institute of Symbiotic Science and Technology, Associate Professor, 大学院・共生科学技術研究部, 助教授 (90158626)
HIKASA Yoshiaki  Tottori University, Faculty of Agriculture, Professor, 農学部, 教授 (30165071)
Project Period (FY) 2002 – 2004
Keywordsmast cell / vascular endothelial cell / extracellular matrix / atopic dermatitis / animal model / cell migration
Research Abstract

Much is known regarding how mast cells contribute to immediate-type and delayed-type allergic inflammations through IgE antibodies. We have already demonstrated that mast cells are derived from multipotential hematopoietic stem cells. However, nobody knows how precursors of mast cells go though the vascular vessels and proliferate at the local tissues in the process of inflammatory responses. We have found production of matrix metalloproteinase-9(MMP-9) by murine bone marrow-derived culture mast cells and human cord blood-derived mast cells. These findings indicate the possible involvement of MMP-9 in proliferation and distribution of mast cells in the local tissues. The aim of the study is to clarify molecular mechanisms for distribution of mast cells in the local tissues focusing on MMP-9. The results obtained by the present study are as follows :
1)IgE crosslinkage of FcεRI induced production and activation of MMP-9 in mast cells.
2)To examine the involvement of cell adhesion molecules in the invasion process of mast cell precursors into endothelial cells, co-culture of mast cell precursors with endothelial cells and fibroblasts were carried out in vitro. Directional movement of mast cells was confirmed in this co-culture system. Furthermore, mast cells with IgE manifested chemotactic movement to its specific antigen and the migration was inhibited by stem cell factor.
3)We found that isolated vascular endothelial cells produced nerve growth factor and expressed its specific receptor ; and the produced nerve growth factor supported survival of vascular endothelial cells. Moreover, nerve growth factor activated mast cells through the collaborative interaction with activated platelets.

  • Research Products

    (25 results)

All 2005 2004 2003 2002

All Journal Article (23 results) Book (2 results)

  • [Journal Article] Mycobacterium vaccae reduces scratching behaviour but not the rash in NC mice with eczema : a randomized, blind, placebo-controlled trial.2005

    • Author(s)
      Arkwright PD, et al.
    • Journal Title

      J.Invest.Dermatol. 124

      Pages: 140-143

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Stem cell factor has a suppressive activity to IgE-mediated chemotaxis of mast cells.2005

    • Author(s)
      Sawada J, et al.
    • Journal Title

      J.Immunol. 174

      Pages: 3626-3632

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A novel NF-κB inhibitor, IMD-0354, suppresses proliferation of human mast cells with constitutively activated c-kit receptors.2005

    • Author(s)
      Tanaka A, et al.
    • Journal Title

      Blood 105

      Pages: 2324-2331

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A new IκB kinase β inhibitor prevents human breast cancer progression through negative regulation of cell cycle transition.2005

    • Author(s)
      Tanaka A, et al.
    • Journal Title

      Cancer Res. (In press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mycobacterium vaccae reduces scratching behavior but not the rash in NC mice with eczema : a randomized, blind, placebo-controlled trial.2005

    • Author(s)
      Arkwright PD, et al.
    • Journal Title

      J.Invest.Dermatol. 124

      Pages: 140-143

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A new IκB kinase βinhibitor prevents human breast cancer progression through negative regulation of cell cycletransition.2005

    • Author(s)
      Tanaka A, et al.
    • Journal Title

      Cancer Res. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A new analytical system for quantitating scratching behaviour in mice.2004

    • Author(s)
      Orito K, et al.
    • Journal Title

      Br.J.Dermatol. 150

      Pages: 1-6

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] IgE alone-induced actin assembly modifies calcium singanling and degranulation in RBL-2H3 mast cells.2004

    • Author(s)
      Oka T, et al.
    • Journal Title

      Am.J.Physiol.Cell Physiol. 286

      Pages: C256-C263

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] An autocrine function of nerve growth factor for cell cycle regulation of vascular endothelial cells.2004

    • Author(s)
      Tanaka A, et al.
    • Journal Title

      Biochem.Biophys.Res.Commun. 313

      Pages: 1009-1014

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] IgE crosslinkage of Fcε receptor I induces both production and activation of matrix metalloproteinase-9 in mast cells.2004

    • Author(s)
      Tanaka A, et al.
    • Journal Title

      Cell.Immunol. 228

      Pages: 66-75

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Time course sequences of angotensin converting enzyme and chymase-like activities during development of right ventricular hypertrophy induced by pulmonary artery constriction in dogs.2004

    • Author(s)
      Orito K, et al.
    • Journal Title

      Life Sci. 75

      Pages: 1135-1145

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A new analytical system for quantitating scratching behavior in mice.2004

    • Author(s)
      Orito K, et al.
    • Journal Title

      Br.J.Dermatol. 150

      Pages: 1-6

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Expression of class II b-tubulin by proliferative myoepithelial cells in canine mammary mixed tumors.2003

    • Author(s)
      Arai K, et al.
    • Journal Title

      Vet.Pathol 40

      Pages: 670-676

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Neuropeptides concentrations in the skin of a murine (NC/Nga mice) model of atopic dermtitis.2003

    • Author(s)
      Katsuno M, et al.
    • Journal Title

      J.Dermatol.Sci. 33

      Pages: 55-65

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Expression of class II b-tubulin by proliferative myoepithelial cells in canine mammary mixed tumors.2003

    • Author(s)
      Arai K, et al.
    • Journal Title

      Vet.Pathol. 40

      Pages: 670-676

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Marked increase in CC chemokine gene expression in both human and mouse mast cell transcriptomes following Fcε receptor I cross-linking : an interspecies comparison.2002

    • Author(s)
      Nakajima T, et al.
    • Journal Title

      Blood 100

      Pages: 3861-3868

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Distribution of the classIi β-tubulin in developmental and adult rat tissues.2002

    • Author(s)
      Arai K, et al.
    • Journal Title

      Cell Motil.Cytoskelton 52

      Pages: 174-182

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A cathelicidin family of human antibacterial peptide LL-37 induces mast cell chemotaxis.2002

    • Author(s)
      Niyonsaba F, et al.
    • Journal Title

      Immunology 106

      Pages: 20-26

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Nerve growth factor activates mast cells through the collaborative interaction with lysophosphatidylserine expressed on the membrane surface of activated platelets.2002

    • Author(s)
      Kawamoto K, et al.
    • Journal Title

      J.Immunol. 166

      Pages: 6412-6419

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Epithelial cell-derived human β-defensin-2 acts as a chemotaxin for mast cells through a pertussis toxin-sensitive and phospholipase, C-dependent pathway.2002

    • Author(s)
      Niyonsaba F, et al.
    • Journal Title

      Int.Immunol. 14

      Pages: 421-426

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Ceramide and sphingosine rapidly induces apoptosis of murine mast cells supported by interleukin 3 and stem cell factor.2002

    • Author(s)
      Itakura A, et al.
    • Journal Title

      Exp.Hematol. 30

      Pages: 272-278

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Marked increase in CC chemokine gene expression in both human and mouse mast cell transcriptomes following Fee receptor I cross-linking : an interspecies comparison.2002

    • Author(s)
      Nakajima T, et al.
    • Journal Title

      Blood 100

      Pages: 3861-3868

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Epithelial cell-derived human β-defensin-2 acts as a chemotaxin for mast cells through a pertussis toxin-sensitive and phospholipase C-dependent pathway.2002

    • Author(s)
      Niyonsaba F, et al.
    • Journal Title

      Int.Immunol. 14

      Pages: 421-426

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Nerve growth factor and wound healing2004

    • Author(s)
      Kawamoto K, et al.
    • Total Pages
      25
    • Publisher
      Elsevier B.V.
    • Description
      「研究成果報告書概要(和文)」より
  • [Book] Animal Models of Human Inflammatory Skin Diseases2004

    • Author(s)
      Kawamoto K, et al.
    • Total Pages
      16
    • Publisher
      CRC Press LLC
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2006-07-11  

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