2005 Fiscal Year Final Research Report Summary
Study of Solanaceous plants from eastern continental temperate climate : collection, evaluation, and molecular phylogeny
| Project/Area Number |
14405023
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
園芸・造園学
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| Research Institution | Chiba University |
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Principal Investigator |
ANDO Toshio Chiba University, Faculty of Horticulture, Professor, 園芸学部, 教授 (30026588)
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| Co-Investigator(Kenkyū-buntansha) |
MII Masahiro Chiba University, Faculty of Horticulture, Professor, 園芸学部, 教授 (30093074)
UEHARA Koichi Chiba University, Faculty of Horticulture, Assistant Professor, 園芸学部, 助手 (20221799)
KOKUBUN Hisashi Chiba University, Center for Environment, Health and Field Science, Associate Professor, 環境健康都市園芸フィールド科学教育研究センター, 助教授 (20282452)
WATANABE Hitoshi Chiba University, Center for Environment, Health and Field Science, Associate Professor, 環境健康都市園芸フィールド科学教育研究センター, 助教授 (80301092)
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| Project Period (FY) |
2002 – 2005
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| Keywords | Molecular phylogeny / Gene resource evaluation / Solanaceae / International research cooperation / Argentina |
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| Research Abstract |
We conducted three expeditions to Argentina to collect Solanaceous plants. In total, 224 accessions were collected, of which 126 were collected as seeds. We sowed 74 accessions, 34 germinated, and 30 reached blooming. We idendified 20 accessions that had ornamental values. All had enough heat and cold tolerance, and thought to be a good breeding materials. Among them, Lycium, Nicotiana, Nierembergia, Solanum were significant genera.
We also conducted phylogenetic study on 24 genera, 35 species plus two outgroups using DNA that was collected during the expeditions. Two intergenic spacers (rpl16-rpl14 and trnT-L-L-F) and an intron (trnK) sequences of chloroplast DNA were determined and phylogenetic trees were calculated from aligned sequences. Argentine Solanaceous plants consisted of five clades that are equivalent to sub families, Schwenckioideae, Cestroideae, Nicotianoideae, Solanoideae, and Petunioideae in addition with a combined clade (Nicotianoideae+Solanoideae) that have been called the "X=12 clade." Sclerophylax was placed outside of Solanaceae+Convolvulaceae clade. Two Patagonian endemic genera, Benthamiella and Pantacantha formed a clade that belonged to Cestroideae. Petunioideae was divided into two subclades, one consists of Bouchetia, Leptoglossis, and Nierembergia and another of Calibrachoa, Fabiana, and Petunia. Petunia patagonica was included in a clade of Fabiana species.
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