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2004 Fiscal Year Final Research Report Summary

Basic and Operational Researches for The Global Elimination of Lymphatic Filariasis

Research Project

Project/Area Number 14406005
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field 寄生虫学(含医用動物学)
Research InstitutionAichi Medical University

Principal Investigator

KIMURA Eisaku  Aichi Medical University, School of Medicine, Professor, 医学部, 教授 (70153187)

Co-Investigator(Kenkyū-buntansha) HIRAYAMA Kenji  Nagasaki Univ., Institute of Tropical Medicine, Professor, 熱帯医学研究所, 教授 (60189868)
ITOH Makoto  Aichi Medical University, School of Medicine, Associate Professor, 医学部, 助教授 (90137117)
TAKAGI Hidekazu  Aichi Medical University, School of Medicine, Assistant, 医学部, 助手 (90288522)
ISOGAI Yoshinori  Nihon Fukushi Univ., Faculty of Social and Information Science, Professor, 情報社会科学部, 教授 (60079697)
Project Period (FY) 2002 – 2005
Keywordsfilariasis / immunodiagnosis / immunoglobulin G4 (IgG4) / urine ELISA / Wuchereria bancrofti
Research Abstract

We have developed a new immunodiagnostic method that uses urine as samples (urine ELISA with the sensitivity of 96.5% and the specificity of 99.0%). The method was tested for the usefulness in field studies.
1.Urine ELISA for measuring the intensity of infection
A big advantage of urine ELISA is its ease in applying the method to young children. They are a sentinel population that can indicate new filarial infections. (We have confirmed that IgG4 transferred from infected mothers to their new born babies disappeared within a year.) In a high endemic area in Sri Lanka, the IgG4 positive appeared at age 1 year, and the antibody titers reached a high level by age 3. In a low endemic area in Thailand, the positive appeared at around 10 years, and showed a high titer level after 20 years of age. In a filariasis-disappeared area in Korea, there was no positive before 40 years of age.
2.Urine ELISA for evaluating Mass Drug Administration (MDA), and confirming elimination of filariasis
Urinary IgG … More 4 levels were studied with schoolchildren in Deniyaya area, Sri Lanka, before and after MDA. After treatment, the positive rate and titers of specific IgG4 reduced clearly, suggesting that urine ELISA is useful to monitor a MDA program. Also in 2 Chinese villages, where filariasis elimination had been confirmed, all of more than 10,000 schoolchildren examined were confirmed negative with the ELISA.
3.Making a filariasis distribution map based on information given by local people
Questionnaires were mailed to local leaders, asking the presence and the number of elephantiasis and hydrocele cases in their villages. The study revealed many villages with the cases, where filariasis had not been reported before. Immunoepidemiological studies using urine ELISA showed a possible filariasis transmission in such villages.
4.Development of new diagnostic methods using recombinant proteins.
Recombinant SXP-1 and endochitinase proteins were used for this study successfully. The former seems to be particularly promising.
5.Genetic analysis of families with more than 1 elephantiasis case
This study is going on and some interesting findings are being accumulated. Less

  • Research Products

    (32 results)

All 2005 2004 2003 2002

All Journal Article (31 results) Book (1 results)

  • [Journal Article] Differential effects of diethylcarbanazine, tetracycline and the compination on Brugia pahangi adult females in vitro2005

    • Author(s)
      Gunawardena NK
    • Journal Title

      Parasitology International 54

      Pages: (253)/(259)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Molecular phylogenetic relationships among seven Japanese species of Cercopithifilaria2005

    • Author(s)
      Agatsuma T
    • Journal Title

      Parasitology International 54

      Pages: (195)/(199)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Strongyloides ratti infection in the large intestine of wild rats, Rattus norvegicus2005

    • Author(s)
      Shintoku Y
    • Journal Title

      Journal of Parasitology 91

      Pages: (1116)/(1121)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Differential effects of diethylcarbamazine, tetracycline and the combination on Brugia pahangi adult females in vitro.2005

    • Author(s)
      Gunawardena N K.
    • Journal Title

      Parasitology International 54

      Pages: 253-259

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Molecular phylogenetic relationships among seven Japanese species of Cercopithifilaria2005

    • Author(s)
      Agatsuma T.
    • Journal Title

      Parasitology International 54

      Pages: 195-199

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Direct agglutination test with urine samples for the diagnosis of visceral leishmaniasis2004

    • Author(s)
      Islam M Z
    • Journal Title

      American Journal of Tropical Medicine and Hygiene 70

      Pages: (78)/(82)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Genotypic variation among lineages of Trypanosoma cruzi and its geographic aspects2004

    • Author(s)
      Higo H
    • Journal Title

      Parasitology International 53

      Pages: (337)/(344)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A unique experimental model for Schistosoma japonicum infection2004

    • Author(s)
      Watanabe K
    • Journal Title

      Parasitology International 53

      Pages: (293)/(299)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Immunogenetic analysis of post-schitosomal liver fibrosis2004

    • Author(s)
      Hirayama K
    • Journal Title

      Parasitology International 53

      Pages: (193)/(196)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Direct agglutination test with urine samples for the diagnosis of visceral leishmamasis.2004

    • Author(s)
      Islam M Z.
    • Journal Title

      American Journal of Tropical Medicine and Hygiene 70

      Pages: 78-82

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Genotypic variation among lineages of Trypanosoma cruzi and its geographic aspects.2004

    • Author(s)
      Higo H.
    • Journal Title

      Parasitology International 53

      Pages: 337-344

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] The miniature pig : A unique experimental model for Schistosoma japonicum infection.2004

    • Author(s)
      Watanabe K.
    • Journal Title

      Parasitology International 53

      Pages: 293-299

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Immunogenetic analysis of post-schitosomal liver fibrosis.2004

    • Author(s)
      Hirayama K.
    • Journal Title

      Parasitology International 53

      Pages: 193-196

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Unexpectedly high prevalence of Wuchereria bancrofti infection obtained by ICT card tests in comparison with that determined by IgG4 ELISA using urine samples : A possibility of false positive reactions with ICT card tests in a study in Thailand2003

    • Author(s)
      Kimura E
    • Journal Title

      Japanese Journal of Tropical Medicine and Hygiene 31

      Pages: (217)/(222)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Bancroftian filariasis in Nepal : a surery for circulating antigenemia of Wuchereria bancrofti and urinary IgG4 antibody in two rural areas of Nepal2003

    • Author(s)
      Watanabe K
    • Journal Title

      Acta Tropica 88

      Pages: (11)/(15)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Human infection with Wuchereria bancrofit in Matara, Sri Lanka : the use, in parallel, of an ELISA to detect filaria-specific IgG4 in urine and of ICT card tests to detect filarial antigen in whole blood2003

    • Author(s)
      Weerasooriya M V
    • Journal Title

      Annals of Tropical Medicine and Parasitology 97

      Pages: (179)/(185)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Prevalence and levels of filaria-specific urinary IgG4 among children less than five years of age and the association of antibody positivity between children and their mothers2003

    • Author(s)
      Weerasooriya M V
    • Journal Title

      American Journal of Tropical Medicine and Hygiene 68

      Pages: (465)/(468)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Sensitive enzyme-linked immunosorbent assay with urine samples : a tool for surveillance of schistosomiasis japonica2003

    • Author(s)
      Itoh M
    • Journal Title

      Southeast Asian Journal of Tropical Medicine and Public Health 34

      Pages: (469)/(472)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Unexpectedly high prevalence of Wuchereria bancrofti infection obtained by ICT card tests in comparison with that determined by IgG4 ELISA using urine samples : A possibility of false positive reactions with ICT card tests in a study in Thailand.2003

    • Author(s)
      Kimura B.
    • Journal Title

      Japanese Journal of Tropical Medicine and Hygiene 31

      Pages: 217-222

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Bancroftian filariasis in Nepal : a survey for circulating antigenemia of Wuchereria bancrofti and urinary IgG4 antibody in two rural areas of Nepal.2003

    • Author(s)
      Watanabe K.
    • Journal Title

      Acta Tropica 88

      Pages: 11-15

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Human infection with Wuchereria bancrofti in Matara, Sri Lanka : the use, in parallel, of an ELISA to detect filaria-specific IgG4 in urine and of ICT card tests to detect filarial antigen in whole blood.2003

    • Author(s)
      Weerasooriya M V.
    • Journal Title

      Annals of Tropical Medicine and Parasitology 97

      Pages: 179-185

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Prevalence and levels of filaria-specific urinary IgG4 among children less than five years of age and the association of antibody positivity between children and their mothers.2003

    • Author(s)
      Weerasooriya M V.
    • Journal Title

      American Journal of Tropical Medicine and Hygiene 68

      Pages: 465-468

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Sensitive enzyme-linked immunosorbent assay with urine samples : a tool for surveillance of schistosomiasis japonica.2003

    • Author(s)
      Itoh M.
    • Journal Title

      Southeast Asian Journal of Tropical Medicine and Public Health 34

      Pages: 469-472

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Effects of Brugia Pahangi infection on the cardiovascular system of stroke-prone spontaneously hypertensive rats2002

    • Author(s)
      Kimura E
    • Journal Title

      Journal of Parasitology 88

      Pages: (839)/(842)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Prevalence and intensity of Wuchereria bancrofti antigenaemia in Sri Lanka by Og4C3 ELISA using filter paper-absorbed whole blood2002

    • Author(s)
      Weerasooriya M V
    • Journal Title

      Transactions of the Royal Society of Tropical Medicine and Hygiene 96

      Pages: (41)/(45)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Diagnosis of visceral leishmaniasis by enzyme-linked immunosorbent assay using urine samples2002

    • Author(s)
      Islam M Z
    • Journal Title

      Clinical and Diagnostic Laboratory Immunology 9

      Pages: (789)/(794)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] High frequency of MIC null haplotype (HLA-B48-MICA-del-MICB^*0107N) in the Angaite Amerindian community in Paraguay2002

    • Author(s)
      Aida K
    • Journal Title

      Immunogenetics 54

      Pages: (439)/441

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Effects of Brugia Pahangi infection on the cardiovascular system of stroke-prone spontaneously hypertensive rats.2002

    • Author(s)
      Kimura E.
    • Journal Title

      Journal of Parasitology 88

      Pages: 839-842

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Prevalence and intensity of Wuchereria bancrofti antigenaemia in Sri Lanka by Og4C3 ELISA using filter paper-absorbed whole blood.2002

    • Author(s)
      Weerasooriya M V.
    • Journal Title

      Transactions of the Royal Society of Tropical Medicine and Hygiene 96

      Pages: 41-45

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Diagnosis of visceral leishmaniasis by enzyme-linked immunosorbent assay using urine samples.2002

    • Author(s)
      Islam M Z.
    • Journal Title

      Clinical and Diagnostic Laboratory Immunology 9

      Pages: 789-794

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] High frequency of MIC null haplotype (HLA-B48-MICA-del-MICB^*0107N) in the Angaite Amerindian community in Paraguay.2002

    • Author(s)
      Aida K.
    • Journal Title

      Immunogenetics 54

      Pages: 439-441

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] Filariasis in Asia and Western Pacific Islands2005

    • Author(s)
      Kimura E
    • Total Pages
      276
    • Publisher
      Federation of Asian Parasitologists
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2007-12-13  

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