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2005 Fiscal Year Final Research Report Summary

Isolation of enterotoxigenic Pseudomonas fluorescens from Asia region

Research Project

Project/Area Number 14406008
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Bacteriology (including Mycology)
Research InstitutionFujita Health University

Principal Investigator

TSUJI Takao  Fujita Health University, School of Medicine, Professor, 医学部, 教授 (60171998)

Co-Investigator(Kenkyū-buntansha) OCHI Sadayuki  Fujita Health University, School of Medicine, Assistant Professor, 医学部, 講師 (80268705)
ARIMITSU Hideyuki  Fujita Health University, School of Medicine, Research Associate, 医学部, 助手 (40367701)
Project Period (FY) 2002 – 2005
KeywordsPseudomonas fluorescens / Enterotoxigenic E coli / heat-labile enterotoxin / Children diarrhea / epidermiology
Research Abstract

We examined whether there was Pseudomonas fluorescens (enterotoxigenic P.fluorescens:ETPF), which produced a toxin like heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli (ETEC), in water at Surabya city in Indoneshia. From many places, we got environmental or house keeping water, isolated P.fluorescens and detected the gene of LT by PCR procedure.
Among about 500 strains, we could get one strain of which chromosome carried the gene like that of LT. Then, we determined the full sequence of LT like gene from ETPF. However, it was almost identical to that of LT of ETEC. Therefore, to order to analyze how its gene in ETPF will be transferred from ETEC, we have been determining the full sequence of chromosomal DNA of ETPF.
Moreover, we tried to isolate ETPF from diarrheal stools of 150 children in Surabaya city. Among them, although we could not isolate pathogenic bacteria like Shigellas, Vibrios, pathogenic E.coli, Campylobacters and ETPF, Pseudomonas, which caused diarrhea in rabbit ileal loop test, were isolated from 6 diarrheal stools. Then, we tried to purify the toxin, and finished its purification. There are many kinds of diarrheal agents of children in developing country, 20 % of diarrheal agents are not determined. Therefore, Pseudomonas isolated in this project may be one of the agents causing children diarrhea in developing country.

  • Research Products

    (16 results)

All 2006 2005 2003

All Journal Article (16 results)

  • [Journal Article] Cleavage specificity of the serine protease of Aeromonas sobria,a member of the kexin family of subtilases.2006

    • Author(s)
      Kobayashi H, Tsuni T et al.
    • Journal Title

      FEMS Microbiology Letters. 256

      Pages: 165-170

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Enhanced mucosal immunogenicity of prion protein following fusion with B subunit of Escherichia coli heat -labile enterotoxin.2006

    • Author(s)
      Yamanaka H, Tsuji T et al.
    • Journal Title

      Vaccine 24

      Pages: 2815-23

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mutant Escherichia coli enterotoxin as a mucosal adjuvant induces specific Th1 responses of CD4(+) and CD8(+) T cells to nasal killed-bacillus calmette-guerin in mice.2006

    • Author(s)
      Takahashi H, Tsuji T et al.
    • Journal Title

      Vaccine 24

      Pages: 3591-8

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Isolation of a human antibody with strong neutralizing activity against diphtheria toxin.2006

    • Author(s)
      Kakita T, Tsuji T, et al.
    • Journal Title

      Infect Immun. (in press)

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A mutant of E, coli enterotoxin inducing a specific Th1-type of T cells to VZV vaccine enhances the production of IL-12 by IFNγ-stimulated MΦ2006

    • Author(s)
      Shimizu T, Sasaki K, Tsuji T. et al.
    • Journal Title

      Vaccine. 24

      Pages: 3719-3726

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Cleavage specificity of the serine protease of Aeromonas sobria, a member of the kexin family of subtilases.2006

    • Author(s)
      Kobayashi H, Takahashi E, Oguma K, Fujii Y, Yamanaka H, Negishi T, Arimoto-Kobayashi S, Tsuji T, Okamoto K.
    • Journal Title

      FEMS Microbiology Letters 256

      Pages: 165-170

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Enhanced mucosal immunogenicity of prion protein following fusion with B subunit of Escherichia coli heat-labile enterotoxin.2006

    • Author(s)
      Yamanaka H, Ishibashi D, Yamaguchi N, Yoshikawa D, Nakamura R, Okimura N, Arakawa T, Tsuji T, Katamine S, Sakaguchi S.
    • Journal Title

      Vaccine. 24(15)

      Pages: 2815-2823

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mutant Escherichia coli enterotoxin as a mucosal adjuvant induces specific Th1 responses of CD4(+) and CD8(+) T cells to nasal killed-bacillus calmette-guerin in mice.2006

    • Author(s)
      Takahashi H, Sasaki K, Takahashi M, Shigemori N, Honda S, Arimitsu H, Ochi S, Ohara N, Tsuji T.
    • Journal Title

      Vaccine. 24(17)

      Pages: 3591-3598

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Isolation of a human antibody with strong neutralizing activity against diphtheria toxin.2006

    • Author(s)
      Kakita M, Takahashi T, Komiya T., Iba Y, Tsuji T, Kurosawa Y, Takahashi M.
    • Journal Title

      Infect.Immun. 74

      Pages: 3682-3683

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Induction of thymus-derived γ δ T cells by Escherichia coli enterotoxin B-subunit in peritoneal cavity of mice.2005

    • Author(s)
      Shimizu T, Tsuji T et al.
    • Journal Title

      Clinical and Diagnostic Laboratory Immunology 12

      Pages: 157-164

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A mutant of Escherichia coli enterotoxin inducing a specific Thl-type of T cells to varicella-zoster vaccine enhances the production of IL-12 by IFNgamma-stimulated macrophages.2005

    • Author(s)
      Shimizu T, Tsuji T et al.
    • Journal Title

      Vaccine 19

      Pages: 1002-1007

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Induction of thymus-derived γδ T cells by Escherichia coli enterotoxin B-subunit in peritoneal cavity of mice.2005

    • Author(s)
      Shimizu T, Sasaki K, Tsuji T. et al.
    • Journal Title

      Clinical and Diagnostic Laboratory Immunology

      Pages: 12157-12164

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Induction of cellular immunity to Varicella-zoster virus glycoproteins tested with pernasal co-administration of Escherichia coli enterotoxin in mice.2003

    • Author(s)
      Tsuji T, Takahashi T, Ochi S, Yokochi T, Asano Y. et al.
    • Journal Title

      J.Med.Virol. 69.451

      Pages: 458

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Live varicella vaccine polarizes the mucosal adjuvant action of CT or CT-B on specific Th1-type helper T cells with a single nasal coadministration in mice.2003

    • Author(s)
      Sasaki K, Tsuji T. et al.
    • Journal Title

      J.Med.Virol. 70

      Pages: 329-335

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Comparison of antimicrobial susceptibility between invasive and non-invasive Shigella organisms.2003

    • Author(s)
      Honma Y, Sasakawa C, Tsuji T et al.
    • Journal Title

      Int J Antimicrob Agents

      Pages: 21262-21266

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Clostridium perfringens alpha-toxin-induced hemolysis of horse erythrocytes is dependent on Ca^<2+> uptake.2003

    • Author(s)
      Ochi S, et al.
    • Journal Title

      Biochim.Biophys.Acta

      Pages: 161379-161386

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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