2004 Fiscal Year Final Research Report Summary
Transcript and metabolite analysis of activation-tagging lines of a model legume
| Project/Area Number |
14540604
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
植物生理
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| Research Institution | Nihon University |
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Principal Investigator |
AOKI Toshio Nihon University, College of bioresource sciences, Associate professor, 生物資源科学部, 助教授 (80287606)
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| Co-Investigator(Kenkyū-buntansha) |
AYABE Shin-ichi Nihon University, College of bioresource sciences, Professor, 生物資源科学部, 教授 (40050679)
AKASHI Tomoyoshi Nihon University, College of bioresource sciences, Research associate, 生物資源科学部, 助手 (80328707)
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| Project Period (FY) |
2002 – 2004
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| Keywords | Lotus japonicus / T-DNA / tagging / mutant / model plant / transformation |
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| Research Abstract |
We constructed T-DNA insertional lines of a model legume, Lotus japonicus, using a multifunctional vector for gene and exon activation tagging. The vector had the CaMV 35S promoter together with two additional enhancer elements, the start codon, and splice donor and acceptor sites facing to the left border, in anticipation of the activation of T-DNA flanking genes and forced expression of flanking exons. The improved transformation technique yielded more than 3,500 lines, including 45 dominant mutant candidates with abnormal phenotypes in aerial parts, nodules and roots. Among selected 44 lines, one copy of T-DNA was inserted into the genome of 37 lines (84%). The T-DNA flanking regions of seven lines were isolated by thermal asymmetric interlaced(TAIL)-PCR or reverse transcription(RT)-PCR, and the corresponding genomic clones were analyzed. The transcripts of four genes adjacent to T-DNA out of eleven genes tested were increased in the T_1 generation, demonstrating that gene and exon activation effects by the newly developed tagging vector are heritable. This is the first report on the activation tagging of a legume, and the T-DNA insertional population of L.japonicus will provide legume-specific dominant mutants.
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Research Products
(15 results)
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[Journal Article] Large-Scale Analysis of Gene Expression Profiles during Early Stages of Root Nodule Formation in a Model Legume, Lotus japonicus2004
Author(s)
Kouchi, H., Shimomura, K., Hata, S., Hirota, A., Wu, G., Kumagai, H., Tajima, S., Suganuma, N., Suzuki, A., Aoki, T., Hayashi, M., Yokoyama, T., Ohyama, T., Asamizu, E., Kuwata, C., Shibata, D., Tabata, S.
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Journal Title
DNA Research 11(4)
Pages: 263-274
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] cDNA cloning and biochemical characterization of S-adenosyl-L-methionine : 2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase, a critical enzyme of the legume isoflavonoid phytoalexin pathway.2003
Author(s)
Akashi, T., Sawada, Y., Shimada, N., Sakurai, N., Aoki, T., Ayabe, S.
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Journal Title
Plant and Cell Physiology 44(2)
Pages: 103-112
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A cluster of genes encodes the two types of chalcone isomerase involved in the biosynthesis of general flavonoids and legume-specific 5-deoxy(iso)flavonoids in Lotus japonicus.2003
Author(s)
Shimada, N., Aoki, T., Sato, S., Nakamura, Y., Tabata, S., Ayabe, S.
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Journal Title
Plant Physiology 131(3)
Pages: 941-951
Description
「研究成果報告書概要(欧文)」より
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