2003 Fiscal Year Final Research Report Summary
Studies on cell signaling for regulating sperm chemotaxis
| Project/Area Number |
14540623
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
動物生理・代謝
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| Research Institution | The University of Tokyo |
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Principal Investigator |
MORISAWA Masaaki The University of Tokyo, Grakuate School of Science, Professor, 大学院・理学系研究科, 教授 (40013594)
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| Co-Investigator(Kenkyū-buntansha) |
TOGO Tatsuru The University of Tokyo, Graduate School of Science, Assistant professor, 大学院・理学系研究科, 助手 (40334247)
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| Project Period (FY) |
2002 – 2003
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| Keywords | ascidians / pacific herring / salmonid fish / chemotacis / activation / initiation / Novel sulfate steroid / Store-operated calcium channel / cyclic AMP / Mathematical treatment |
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| Research Abstract |
On the studies of sperm chemotaxis and activation in the ascidians Ciona 1) molecular structure of sperm-activating and -attracting factor (SAAF) originated from the eggs was determined using NMR and MS/MS methods. The factor is the novel steroid : (3R,4R,7R,25S)-3,4,7,26-tetrahydroxycholestane-3,26-disulfate. The steroid was synthesized and it was demonstrated that nanomolar concentration could activate and attract the ascidian sperm. 2) In the cell signaling for the sperm chemotaxis, it was demonstrated that stor-operated calcium channel plays key role for the phenomenon. Furthermore, a numerical studies showed that two different strategies cause sperm chemotaxis in the ascidians and siphonophores. 3) In the cell, signaling for the activation of ascidian sperm, It was demonstrated that calcium/calmodulin/calmoduline kinase system activates K^+ channel and resulting membrane hyperpolarization causes cAMP increase, resulting in the activation of sperm motility through phosphorylation of axonemal proteins. 4) On the studies of sperm activation in the Pacific hering Clupea pallasi, participation of reversed Na^+/Ca^<2+> exchanger was clarified. 5) On the cell signaling for the, initiation of sperm motility in the salmonid fish, cAMP-dependent protein kinase catalytic subunit was characterized. Furthermore, the 15kDa protein, which is known to be indispensable for the initiation of flagellar motility, was purified, and it's characterized. The acrosome reaction of spermatozoa was first found in amphioxus in the world, and participation of GTP-anchored protein in the acrosome reaction was also first found the mussel, Mytilus edulis.
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Research Products
(19 results)
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[Journal Article] Purification and characterization of the 15-K protein from the sperm flagella of salmonid fihes2003
Author(s)
Itoh, A., Fujinoki, M., Kawahara, T., Inaba, K., Shimizu, N., Morisawa, M.
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Journal Title
Biomedical Research 24
Pages: 153-164
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Chracterization of a camp-dependent protein kinase catalytic subunit from rainbow trout spermatozoa2003
Author(s)
Itoh, A., Inaba, K., Ohotake, H., Fujinoki, K., Morisawa, M.
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Journal Title
Biochem.Biophys.Res.Comn., 305
Pages: 855-861
Description
「研究成果報告書概要(和文)」より
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[Journal Article] Purification and characterization of the 15-kDa protein from the sperm flagella of salmonid fishes.2003
Author(s)
Itoh A., Fujinoki, M., Kawamura, T., Inaba, K., Shimizu, N., Morisawa, M.
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Journal Title
Biomed.Res. 24
Pages: 153-164
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Motility initiation in ferring sperm is regulated by reverse sodium-calcium exchange.2002
Author(s)
Vines, C.A., Yoshida, K., Griffin, F.J., Pillai, M.C., Morisawa, M., Yanagimachi, R., Cherr, G.N.
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Journal Title
Proc.Natl.Acad.Sci.USA. 99
Pages: 2026-2031
Description
「研究成果報告書概要(欧文)」より
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[Book] 基礎生物学2003
Author(s)
森沢 正昭
Total Pages
227
Publisher
NHK出版
Description
「研究成果報告書概要(和文)」より
