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2003 Fiscal Year Final Research Report Summary

Studies on cell signaling for regulating sperm chemotaxis

Research Project

Project/Area Number 14540623
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 動物生理・代謝
Research InstitutionThe University of Tokyo

Principal Investigator

MORISAWA Masaaki  The University of Tokyo, Grakuate School of Science, Professor, 大学院・理学系研究科, 教授 (40013594)

Co-Investigator(Kenkyū-buntansha) TOGO Tatsuru  The University of Tokyo, Graduate School of Science, Assistant professor, 大学院・理学系研究科, 助手 (40334247)
Project Period (FY) 2002 – 2003
Keywordsascidians / pacific herring / salmonid fish / chemotacis / activation / initiation / Novel sulfate steroid / Store-operated calcium channel / cyclic AMP / Mathematical treatment
Research Abstract

On the studies of sperm chemotaxis and activation in the ascidians Ciona 1) molecular structure of sperm-activating and -attracting factor (SAAF) originated from the eggs was determined using NMR and MS/MS methods. The factor is the novel steroid : (3R,4R,7R,25S)-3,4,7,26-tetrahydroxycholestane-3,26-disulfate. The steroid was synthesized and it was demonstrated that nanomolar concentration could activate and attract the ascidian sperm. 2) In the cell signaling for the sperm chemotaxis, it was demonstrated that stor-operated calcium channel plays key role for the phenomenon. Furthermore, a numerical studies showed that two different strategies cause sperm chemotaxis in the ascidians and siphonophores. 3) In the cell, signaling for the activation of ascidian sperm, It was demonstrated that calcium/calmodulin/calmoduline kinase system activates K^+ channel and resulting membrane hyperpolarization causes cAMP increase, resulting in the activation of sperm motility through phosphorylation of axonemal proteins. 4) On the studies of sperm activation in the Pacific hering Clupea pallasi, participation of reversed Na^+/Ca^<2+> exchanger was clarified. 5) On the cell signaling for the, initiation of sperm motility in the salmonid fish, cAMP-dependent protein kinase catalytic subunit was characterized. Furthermore, the 15kDa protein, which is known to be indispensable for the initiation of flagellar motility, was purified, and it's characterized. The acrosome reaction of spermatozoa was first found in amphioxus in the world, and participation of GTP-anchored protein in the acrosome reaction was also first found the mussel, Mytilus edulis.

  • Research Products

    (19 results)

All 2004 2003 2002

All Journal Article (18 results) Book (1 results)

  • [Journal Article] Strategies for sperm chemotaxis in the siphonophors and ascidians : a numerical study2004

    • Author(s)
      Ishikawa M., Tsutsui H., Cosson J., Oka Y., Morisawa M.
    • Journal Title

      Biological Bulletin 206

      Pages: 95-102

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Calmodulin/calmodulin-dependent protein kinase II mediates SAAF-induced activation of ascidian sperm.2004

    • Author(s)
      Nomura, M., Yoshida, M., Morisawa, M.
    • Journal Title

      J. Cytoskelt. Cell Motil. 59

      Pages: 28-37

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Synthesis and identification of an endogenous sperm activating and atracting factor isolated from eggs of the ascidian Ciona intestinalis ; an example of nanomolar-level structure elucidation of novel natural compound..2004

    • Author(s)
      Oishi, T., Tsuchikawa, H., Murata, M., Yoshida, M., Morisawa, M.
    • Journal Title

      Tetrahedron 60

      Pages: 6971-6980

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Strategies for sperm chemotaxis in the siphonophors and ascidians : a numerical study.2004

    • Author(s)
      Ishikawa M., Tsutsui H., Cosson J., Oka Y., Morsawa, M.
    • Journal Title

      Biol.Bull. 206

      Pages: 95-102

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Calmodulin/calmodulin-dependent protein kinase II mediates SAAF-induced activation of ascidian sperm.2004

    • Author(s)
      Nomura M., Yoshida M., Morisawa M.
    • Journal Title

      J.Cytoskelt.Cell Motile. 59

      Pages: 28-37

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Synthesis and identification of an endogenous sperm activating and atracting factor isolated from eggs of the ascidian Ciona intestinalis ; an example of nanomolar-level structure elucidation of novel natural compound.2004

    • Author(s)
      Oishi, T., Tsuchikawa, H., Murata, M., Yoshida, M., Morisawa, M.
    • Journal Title

      Tetrahedron 60

      Pages: 6971-6980

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Store-operated calcium channel regulates chemotactic behavior of ascidian sperm.2003

    • Author(s)
      Yoshida, M., Ishikawa, M., Izumi, H., De Santis, R., Morisawa, M.
    • Journal Title

      Proceedings National Academy of Science USA 100

      Pages: 149-154

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Purification and characterization of the 15-K protein from the sperm flagella of salmonid fihes2003

    • Author(s)
      Itoh, A., Fujinoki, M., Kawahara, T., Inaba, K., Shimizu, N., Morisawa, M.
    • Journal Title

      Biomedical Research 24

      Pages: 153-164

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Chracterization of a camp-dependent protein kinase catalytic subunit from rainbow trout spermatozoa2003

    • Author(s)
      Itoh, A., Inaba, K., Ohotake, H., Fujinoki, K., Morisawa, M.
    • Journal Title

      Biochem.Biophys.Res.Comn., 305

      Pages: 855-861

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Synthesis of endogenous sperm-activating and -attracting factor isolated from egg of ascidian Ciona intestinalis.2003

    • Author(s)
      Oishi, T., Tsuchikawa, H., Murata, M., Yoshida, M., Morisawa, M
    • Journal Title

      Tetrahedron Lett. 44

      Pages: 6387-6389

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Store-operated calcium channel regulates chemotactic ] behavior of ascidian sperm.2003

    • Author(s)
      Yoshida, M., Ishikawa, M., Izumi, H., De Santis, R., Morisawa, M.
    • Journal Title

      Proc.Natl.Acad Sci.USA 100

      Pages: 149-154

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Purification and characterization of the 15-kDa protein from the sperm flagella of salmonid fishes.2003

    • Author(s)
      Itoh A., Fujinoki, M., Kawamura, T., Inaba, K., Shimizu, N., Morisawa, M.
    • Journal Title

      Biomed.Res. 24

      Pages: 153-164

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Characterization of a cAMP-dependent protein kinase catalytic subunit from rainbow trout spermatozoa.2003

    • Author(s)
      Itho, A., Inaba, K., Ohtake, H., Fujinoki, M., Morisawa, M.
    • Journal Title

      Biochem.Biophys.Res.Comn. 305

      Pages: 855-861

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Synthesis of endogenous sperm-activating and attracting factor gg. isolated from ascidian Ciona intestinalis.2003

    • Author(s)
      Oishi, T., Tsuchikawa, H., Murata, M., yohida, M., Morisawa, M.
    • Journal Title

      Tetrahedron Lett. 44

      Pages: 6387-6389

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] A chemoattractant of the ascidian spermatozoa is a novel sulfated steroid.2002

    • Author(s)
      Yoshida, M., Murata, M., Inaba, K, Morisawa, M
    • Journal Title

      Proceedings National Academy of Science USA 99

      Pages: 14831-14836

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Motility initiation in herring sperm is regulated by reverse sodium-calcium exchange.2002

    • Author(s)
      Vines, C.Yashida, K., Griffin, Pillai, MC.Morisawa M., Yanagimachi, R., Cherr, G.
    • Journal Title

      Proceedings national Academy of Science USA 99

      Pages: 2026-2031

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] A chemoattractant of the ascidian spermatozoa is a novel sulfated steroid.2002

    • Author(s)
      Yoshida, M., Murata, M., Inaba, K., Morisawa, M.
    • Journal Title

      Proc.Natl.Acad.Sci.USA. 99

      Pages: 14831-14836

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Motility initiation in ferring sperm is regulated by reverse sodium-calcium exchange.2002

    • Author(s)
      Vines, C.A., Yoshida, K., Griffin, F.J., Pillai, M.C., Morisawa, M., Yanagimachi, R., Cherr, G.N.
    • Journal Title

      Proc.Natl.Acad.Sci.USA. 99

      Pages: 2026-2031

    • Description
      「研究成果報告書概要(欧文)」より
  • [Book] 基礎生物学2003

    • Author(s)
      森沢 正昭
    • Total Pages
      227
    • Publisher
      NHK出版
    • Description
      「研究成果報告書概要(和文)」より

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Published: 2006-07-11  

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