2003 Fiscal Year Final Research Report Summary
Development of the multi-ion-imaging sensor and its application to biological specimens
| Project/Area Number |
14550791
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
工業物理化学
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| Research Institution | Osaka University |
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Principal Investigator |
YOSHINOBU Tatsuo Osaka University, The Institute of Scientific and Industrial Research, Associate Professor, 産業科学研究所, 助教授 (30243265)
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| Co-Investigator(Kenkyū-buntansha) |
IWASAKI Hiroshi Osaka University, The Institute of Scientific and Industrial Research, Professor, 産業科学研究所, 教授 (00029901)
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| Project Period (FY) |
2002 – 2003
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| Keywords | sensor / chemical sensor / ion / semiconductor device / LAPS / chemical imaging sensor |
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| Research Abstract |
1. Development of multi-ion-imaging technology
The conditions for the fabrication of ion-sensitive membranes with ionophores were optimized to implement various ion sensitivities on the sensing surface of the light-addressable potentiometric sensor (LAPS). A photo-patterning process was developed for the integration of ion-sensitive photocurable membranes on a single sensor chip. The distribution of the ion concentration was visualized with a sensor with integrated K^+-and Ca^<2+>-sensitive membranes.
2. Development of Enzyme LAPS
For the application of the LAPS and the chemical imaging sensor to biological specimens, enzyme LAPS was developed with urease and butyrylcholinesterase. Various methods for the immobilization of enzyme molecules on the sensor surface were compared in terms of sensitivity and stability.
3. Development of a pen-shaped portable LAPS device
To allow more flexible use of LAPS; a pen-shaped portable LAPS device was developed. The control and measurement circuits are built in the device, which can be directly dipped into the solution to be measured. The sensor chip has 4 or 16 measurement points on the surface, each of which can be independently assigned for measurement of different ion species.
4. Application to the microfluidic devices
For the application to the detection and measurement of chemical species in the microfluidic devices such as μTAS and Lab-on-a-Chip, a LAPS-based system was combined with a microfluidic channels fabricated with the SU-8/PDMS technology. This system allows label-free measurement of ion concentration at an arbitrary point in the microchannel. The distribution of the ion concentration in the microchannel was visualized.
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Research Products
(16 results)
