2003 Fiscal Year Final Research Report Summary
Synthesis of Dendrimer-Type AIDS Vaccine having Cyclic Peptide Antigen
| Project/Area Number |
14550840
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
高分子合成
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| Research Institution | Teikyo University of Science and Technology |
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Principal Investigator |
URYU Toshiyuki Teikyo University of Science and Technology, Faculty of Science and Engineering, Professor, 理工学部, 教授 (80011005)
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| Co-Investigator(Kenkyū-buntansha) |
KATSURAYA Kaname Wayo Women's University, Faculty of Domestic Science, Associate Professor, 家政学部, 助教授 (20251465)
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| Project Period (FY) |
2002 – 2003
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| Keywords | Dendrimer-type AIDS vaccine / Spherical polylysine dendrimer / Spherical polyornithine dendrimer / Monodispersed dendrimer / Glyco-peptide dendrimer / HIV sequential peptide / Reductive amination / Carboxylated dendrimer |
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| Research Abstract |
Synthesis of spherical dendrimer-type AIDS vaccine was investigated. Spherical polyl(ysine) dendrimer generation three (G3), which can be regarded as a didendron, was synthesized starting from 1,4-diaminobutane as a core compound. β-Alanine was bound to the terminal amino group of the dendrimer to afford pseudo poly(lysine) dendrimer G4. When β-alanine-poly(lysine) dendrimer was reacted by reductive amination with such oligosaccharides as lactose, maltose, and cellobiose, the resulting oligosaccharide-β-alanine-poly(lysine) dendrimer showed monodispersed molecular weight distribution like natural proteins. The oligosaccharide-β-alanine-poly(lysine) dendrimer had 32 oligosaccharide units the two each of which were connected to the terminal 16 amino groups of the β-alanine-poly(lysine) dendrimer, showing a molecular weight of approximately 13453 close to the theoretical molecular weight. On the other hand, when poly(ornithine) dendrmer was used instead of poly(lysine) dendrimer, sperical oligosaccharide-β-alanine-poly(rnithine) dendrimers showed the molecular weight of 11900 to 13200 and the number of substituted oligosaccharides of 28 to 32. Furthermore, when hemispherical proline-poly(lysine) dendrimer was prepared and reacted with maltose, maltose-proline-poly(lysine) dendrimer having a monodispersed molecular weight of approximately 4400 was obtained. Furthermore, succinyl-maltose-proline-poly(lysine) dendrimer was synthesized and reacted with linear polypeptide antigen from AIDS virus consisting of 24 amino residues to give a dendrimer-type AIDS vaccine candidate in which one to two antigen peptides were bound to the succinyl group through amido linkage.
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