2003 Fiscal Year Final Research Report Summary
Functional analysis of Nck-interacting kinase (NIK)-related kinase (NRK) in vivo.
| Project/Area Number |
14560257
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Kyorin University |
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Principal Investigator |
KANAI Masami Kyorin University, Medical School, Assistant Professor, 医学部, 助手 (70321883)
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| Co-Investigator(Kenkyū-buntansha) |
KAWAKAMI Hayato Kyorin University, Medical School, Professor, 医学部, 教授 (30146542)
HAYASHI Yoshihiro Kyorin University, Faculty of Agriculture, Professor, 大学院・農学生命科学研究科, 教授 (90092303)
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| Project Period (FY) |
2002 – 2003
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| Keywords | Skeletal muscle development / kinase / GCK / Transgenic mouse / MAP4K |
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| Research Abstract |
Nck-interacting kinase (NIK)-related kinase (NRK) is a protein kinase that belongs to the germinal center kinase (GCK) family, and active the c-Jun N-terminal kinase (JNK) signaling pathway. In this study, we examined the effect of NRK on actin cytoskeletal organization in mouse muscle development. Overexpression of NRK in COS7 cells induced accumulation of polymerized actin at the perinuclear. Phosphorylation of cofilin, an actin-depolymerized factor, was increased in NRK-expressing HEK 293T cells. In addition, in vitro phosphorylation occurred at the serine residue of position 3 (Ser-3). Since the phosphorylation at Ser-3 inactives the actin-depolymerizing activitiy of cofilin, these results suggest that NRK induces actin polymerization through cofilin kinase, or through the activation of JNK. Thus, cofilin is likely to be a direct substrate o NRK. NRK is predominantly expressed in skeletal muscle during late stage of mouse embryogenesis. Thus, NRK may be involved in the regulation of actin cytoskeletal organization in skeletal muscle cells through cofilin phosphorylation. We have made the transgenic mouse that constracted Nrk or dominant negative Nrk (Nrk-K54 E) at the downstream of myogenin promoter, which overlapped the expression pattern with Nrk. Further studies are needed to clarify the involvement of NRK in the activity of cofilin and the regulation of actin assembly in skeletal muscle cells during mammalian embryogenesis.
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[Publications] Kotani M., Tajima Y., Osanai T., Irie A., Iwatsuki K., Kanai-Azuma M., Imada M., Kato H., Shitara H., Kubo H., Sakuraba H.: "Complementary DNA cloning and characterization of RANDAM-2, a type I membrane molecule specifically expressed on glutamatergic neuronal cells in the mouse cerebrum."J Neurosci Res. 73. 603-613 (2003)
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[Publications] Noma T., Kanai Y., Kanai-Azuma M., Ishii M., Fujisawa M., Kurohmaru M., Kawakami H., Wood SA., Hayashi Y.: "Stage-and sex-dependent expressions of Usp9x, and X-linked mouse orthologue of Drosophila Fat facets, during gonadal development and oogenesis in mice."Mech.Dev.. 119(suppl 1). s91-s95 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Kanai-Azuma M., Kanai Y., Gad J.M., Tajima Y., Taya C., Kurohmaru M., Sanai Y., Yonekawa H., Yazaki K., Tam L., Hayashi Y.: "Depletion of definitive gut endoderm in Sox17-null mutant mice."Development. 129(10). 2367-2379 (2002)
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「研究成果報告書概要(欧文)」より
