2004 Fiscal Year Final Research Report Summary
Oprimization of production system for bioplastics by enzyme evolutionary engineering
| Project/Area Number |
14560285
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
生物資源科学
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| Research Institution | Hokkaido University (2004)
Meiji University (2002-2003) |
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Principal Investigator |
TAGUCHI Seiichi Hokkaido Univ., Graduate School of Engineering, Professor, 大学院・工学研究科, 教授 (70216828)
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| Co-Investigator(Kenkyū-buntansha) |
HIRAISHI Tomohiro Institute of RIKEN, Scientist, 研究員 (20321804)
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| Project Period (FY) |
2002 – 2004
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| Keywords | biodegradable plastics / polyhydroxybuthyrate / evolutionary engineering / polymerase / substrate specificity / monomer composition |
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| Research Abstract |
Biotechnological studies for biosynthesis of polyhydroxyalkanoates (PHAs) biopolyester have been extensively progressed through the development of various metabolic engineering strategies involving fermentation technology of naturally occurring PHA-producing bacteria based on external substrate manipulation (1st generation), subsequently reinforced with recombinant gene technology (2nd generation). Most recently, "enzyme evolution" is becoming the 3rd generation approach for PHA production. Break-through in chemical synthesis of macromolecules with desirable properties was achieved by development of prominent chemical catalysts, "catalyst evolution", as represented by a series of Zigler-Natta catalysts. Thus, one can easily accept the concept that molecular evolution of the biocatalysts (enzymes) relevant to PHA synthesis will provide us a chance to create novel PHA materials with high-performance. The first trial of the in vitro enzyme evolution in PHA biosynthesis was reported by our group in 2001. The following literature data as well as our own experimental results devoted to this new approach have been gradually accumulated for a short time.
This research project focused specifically on the concept and current case studies of the application of "enzyme evolution" to PHA biosynthesis.
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Research Products
(23 results)
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[Journal Article] Characterization and properties of G4X mutants ofRalstonia eutropha PHA synthase for poly(3-hydroxybutyrate) biosynthesis in Escherichia coli.2005
Author(s)
Normi, Y.M., Hiraishi, T., Taguchi, S., Abe, H., Sudesh.K, Najimudin, Doi, Y.
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Journal Title
Macromol.Biosci. 5(3)
Pages: 197-206
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Biosynthesis of polyhydroxyalkanoate (PHA) copolymer from fructose using wild-type and laboratory-evolved PHA synthases.2005
Author(s)
Tsuge, T., Yano, K., Imazu, S., Numata, K., Kikkawa, Y., Abe, H., Taguchi, S., Doi, Y.
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Journal Title
Macromol.Biosci. 5(2)
Pages: 112-117
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Production of polyhydroxyalkanoate (PHA) from renewable carbon sources in recombinant Ralstonia eutropha using mutants of original PHA synthase.2003
Author(s)
Taguchi, S., Nakamura, H., Kichise, T., Tsuge, T., Yamato, I., Doi, Y.
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Journal Title
Biochem.Eng.J. 16(2)
Pages: 107-113
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Correlation between structure of the lactones and substrate specificity in enzyme-catalyzed polymerization for the synthesis of polyesters.2003
Author(s)
Suzuki, Y., Taguchi, S,, Hisano, T., Toshima, K., Matsumura, H., Doi, Y.
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Journal Title
Biomacromolecules 2(3)
Pages: 537-543
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Biosynthesis of biodegradable copolyesters from renewable carbon sources by recombinant bacteria.2002
Author(s)
Taguchi, S., Matsusaki, H., Matsumoto, K., Takase, K., Taguchi, K., Doi, Y.
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Journal Title
Polymer.Internat. 51(10)
Pages: 899-906
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] A repressor protein, PhaR, regulates PHA synthesis via its direct interaction with PHA.2002
Author(s)
Maehara, A., Taguchi S., Nishiyama, T., Yamane, T, Doi, Y.
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Journal Title
J.Bacteriol. 184(14)
Pages: 3992-4002
Description
「研究成果報告書概要(欧文)」より
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