2003 Fiscal Year Final Research Report Summary
Three-dimensional localization of a gamma-tubulin-like molecule in the enterocyte of the mouse duodemun and its molecular analysis
| Project/Area Number |
14570014
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General anatomy (including Histology/Embryology)
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
SUZAKI Etsuko Hiroshima University, Graduate School of Biomedical Sciences, Assistant Professor, 大学院・医歯薬学総合研究科, 講師 (10274052)
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| Co-Investigator(Kenkyū-buntansha) |
KATAOKA Katsuko Hiroshima University, Graduate School of Biomedical Sciences, Professor, 大学院・医歯薬学総合研究科, 教授 (30034002)
SAITO Akira Hiroshima University, Graduate School of Biomedical Sciences, Research Associate, 大学院・医歯薬学総合研究科, 助手 (80346486)
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| Project Period (FY) |
2002 – 2003
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| Keywords | gamma-tubulin / microtubule / cell adhesion molecule / cells-meeting junction / three-dimensional analysis / proteome analysis / NOXO 1 / immunoelectron microscopy |
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| Research Abstract |
Microtubules play important roles in maintaining the cell morphology and polarity. We had been studying to clarify the localization of gamma-tubulin in a polarized cell which organizes microtubule arrays and found that one of anti-gamma-tubulin antibody, G9, stained three-cells meeting junctions in the mouse duodenal enterocyte. Therefore, we have investigated the precise localization of G9-staining molecule and its molecular property for the two years of 2002-2003
G9 staining was examined by using a confocal microscope, and it was shown that G9 stained three-cells-junction in the middle to upper part of lateral membranes. Especially, the staining of the top regions were strong but was totally different from the one of E-cadherin. Immunoelectronmicroscopy was further performed, and the labels of an anti-gamma-tubulin antibody and G9 were seen around the three-cells-meeting junctions where the labels of an anti-alfa-tubulin antibody were also detected. These results confirmed that a gamma-tubulin-like molecule localizes at three-cells-meeting junction associating with microtubules
In order to clarify G9-recognising molecule, the proteome analysis and succeeding peptide-MASS-fingerprint analysis was also performed. The proteome analysis demonstrated that there was one candidate which would be valuable for the further analysis. The peptide-MASS-fingreprint analysis suggests that the G9-recognizing molecule is possibly NOXO (NASPH-oxidase organizer) 1 whose c-DNA was newly identified in 2003. Further investigations will be needed to work out more details
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