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2004 Fiscal Year Final Research Report Summary

Establishment of an in vitro co-culture system for the renal glomerular filtration barier.

Research Project

Project/Area Number 14570015
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionEhime University

Principal Investigator

KOBAYASHI Naoto  Ehime University, School of Medicine, Associate Professor, 医学部, 助教授 (50234836)

Co-Investigator(Kenkyū-buntansha) SAITO Shouichiro  Gifu University, Faculty of Applied Biological Sciences, Associate Professor, 応用生物科学部, 助教授 (60325371)
Project Period (FY) 2002 – 2004
Keywordskidney / glomerulus / glomerular filtration barrier / podocyte / endothelial cell / cell culture / cytoskeleton / cell adhesion
Research Abstract

The initial step of the urine generation in the kidney is the glomerular filtration through the glomerular filtration barrier, which prevents protein leakage into the urine. In order study the pathophysiology of glomerular diseases, where the ultrastructure of the glomerular filtration barrier is damaged, it is necessary to establish a reliable model system to reproduce the structure and function of the barrier. The filtration barrier is composed of three components, i.e. the glomerular capillary endothelial cell, the glomerular basement membrane, and the podocyte, also known as the glomerular epitheliaI cell. This study has aimed to establish such a model using cell culture system.
With the conditionally immortalized mouse podocyte cell line which is kindly provided by our foreign collaborators, we have studied the signal transduction pathway regulating the podocyte morphogenesis (both process formation and cell-cell contact formation) with special reference on the RHO-family small GTP … More ases (RhoA, Rac1, Cdc42) (Kobayashi N, 2002 ; Kobayashi et al., 2004 ; Gao et al., 2004 ; Gao et al., manuscript in preparation). Since in in vivo glomeruli, podocytes develop a cell-type specific intercellular junction, slit membrane, the formation of intercellular junctions between podocytes is thought to be the critical step in the development of the filtration barrier. We have shown that tight cell-cell contacts are induced between cultured podocytes by inhibition of RhoA with forskolin. Biochemical approach revealed the activation of other members of the RHO-family, Rac1 and Cdc42, during this cell-cell contact formation. It seems unlikely that a co-culture system (podocytes + endothelial cells) is absolutely necessary to establish the glomerular filtration barrier in vitro. We have also shown that pharmacological inhibition of RhoA and its downstream effector, ROCK, enhances process formation of the cultured podocytes. As planned at the beginning of this study, we are still trying to establish cell lines of renal glomerular endothelial cells. Less

  • Research Products

    (19 results)

All 2004 2003 2002

All Journal Article (19 results)

  • [Journal Article] Process formation of the renal glomerular podocyte : Is there common molecular machinery for processes of podocytes and neurons?2004

    • Author(s)
      Kobayashi N, Saito S, et al.
    • Journal Title

      Anatomical Science International 79・1

      Pages: 1-10

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Rho-ROCK signal pathway regulates microtubule-based process formation of cultured podocytes-inhibition of ROCK promoted process elongation.2004

    • Author(s)
      Gao S, Saito S, Kobayashi N, et al.
    • Journal Title

      Nephron Experimental Nephrology 97・2

      Pages: e49-e61

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Transient increase of TUNEL-positive cells on postnatal day 20 in the developing rat olfactory bulb.2004

    • Author(s)
      Saito K, Saito S, Kobayashi N, et al.
    • Journal Title

      Neuroscience Research 50・2

      Pages: 219-225

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Differetiation-induced cultured podocytes express endocytically active megalin, a Heymann nephritis antigen.2004

    • Author(s)
      Yamazaki H, Kobayashi N, et al.
    • Journal Title

      Nephron Experimental Nephrology 96・2

      Pages: e52-e58

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Process formation of the renal glomerular podocyte : Is there common molecular machinery for processes of podocytes and neurons?2004

    • Author(s)
      Kobayashi N, Saito S, et al.
    • Journal Title

      Anatomical Science International 79

      Pages: 1-10

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Rho-ROCK signal pathway regulates microtubule-based process formation of cultured podocytes -inhibition of ROCK promoted process elongation.2004

    • Author(s)
      Gao S, Saito S, Kobayashi N, et al.
    • Journal Title

      Nephron Experimental Nephrology 97

      Pages: e49-e61

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Transient increase of TUNEL-positive cells on postnatal day 20 in the developing rat olfactory bulb.2004

    • Author(s)
      Saito K, Saito S, Kobayashi N, et al.
    • Journal Title

      Neuroscience Research 50

      Pages: 219-225

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Differentiation-induced cultured podocytes express endocytically active megalin, a Heymann nephritis antigen.2004

    • Author(s)
      Yamazaki H, Kobayashi N, et al.
    • Journal Title

      Nephron Experimental Nephrology 96

      Pages: e52-e58

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] An anomalous flexor of the little finger : case report.2003

    • Author(s)
      Kobayashi N, Saito S, et al.
    • Journal Title

      Clinical Anatomy 16

      Pages: 40-43

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Lectin-histochemical studies on the olfactory organ of the newt, Cynops pyrrhogaster.2003

    • Author(s)
      Saito S, Kobayashi N, et al.
    • Journal Title

      Anatomy and Embryology 23・2

      Pages: 349-356

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Distribution pattern of β-catenin during the early embryogenesis of the starfish, Asterina pectinifera.2003

    • Author(s)
      Miyawaki K, Kobayashi N, Saito S, et al.
    • Journal Title

      Development Growth and Differentiaion 45

      Pages: 121-128

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Preparation and Characteriation of recombinant murine p65/L-plastin expressed in Escherichia coli and high-titer antibodies against the protein.2003

    • Author(s)
      Shinomiya H, Kobayashi N, et al.
    • Journal Title

      Bioscience Biotechnology Biochemistry 67

      Pages: 1368-1375

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Interferon-inducible protein 10 (IP-10/CXCL10) has a novel potential ability in maintaining podocyte function.2003

    • Author(s)
      Han GD, Kobayashi N, et al.
    • Journal Title

      Journal of American Society of Nephrology 14

      Pages: 3111-3126

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Lectin-histochemical studies on the olfactory organ of the newt, Cynops pyrrhogaster.2003

    • Author(s)
      Saito S, Kobayashi N, et al.
    • Journal Title

      Anatomy and Embryology 23

      Pages: 349-356

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Distribution pattern of β-catenin during the early embryogenesis of the starfish, Asterina pectinifera.2003

    • Author(s)
      Miyawaki K, Saito S, Kobayashi N, et al.
    • Journal Title

      Development Growth Differentiation 45

      Pages: 121-128

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Preparation and Characterization of recombinant murine p65/L-plastin expressed in Escherichia coli and high-titer antibodies against the protein.2003

    • Author(s)
      Shinomiya H, Kobayashi N, et al.
    • Journal Title

      Bioscience Biotech Biochem 67

      Pages: 1368-1375

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Interferon-inducible protein 10 (IP-10/CXCL10) has a novel potential ability in maintaining podocyte function.2003

    • Author(s)
      Han GD, Kobayashi N, et al.
    • Journal Title

      J American Society of Nephrology 14

      Pages: 3111-3126

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Mechanism of the process formation : podocytes versus neurons.2002

    • Author(s)
      Kobayashi N
    • Journal Title

      Microscopy Research and Technique 57・4

      Pages: 217-223

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Mechanism of the process formation; podocytes versus neurons.2002

    • Author(s)
      Kobayashi N
    • Journal Title

      Microscopy Research and Technique 57

      Pages: 217-223

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2006-07-11  

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