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2003 Fiscal Year Final Research Report Summary

Identification of nuclear proteins involved in cardiac hypertrophy caused by abnormality of fatty acid metabolism

Research Project

Project/Area Number 14570124
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pathological medical chemistry
Research InstitutionKagoshima University

Principal Investigator

HORIUCHI Masahisa  Kagoshima Uniyersity, Graduate School of Medical and Dental Sciences, Assistant Professor, 大学院・医歯学総合研究科, 講師 (50264403)

Co-Investigator(Kenkyū-buntansha) SAHEKI Takeyori  Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院・医歯学総合研究科, 教授 (10056070)
Project Period (FY) 2002 – 2003
KeywordsCarnitine / JVS mice / cAMP responsive element / Cardiac hypertrophy / Prostaglandin / medium-chain triglyceride / CDV-1 / CDV-3
Research Abstract

To understand the molecular mechanisms of cardiac hypertrophy in carnitine-deficient mice, we examined the mice as follows ;
1)The cardiac hypertrophy was markedly attenuated by decreasing the soy bean oil(SBO)content from 5 to 1%.Addition of medium-chain triglyceride which can be metabolically utilized by JVS mice did not affect the development of cardiac hypertrophy.On the otherhand, the mRNA levels of CDV-1 of JVS mice fed 5% and 1% SBO diet showed significantly lower values than controls fed the same diet, respectively.There were no significant difference in ATP content and energy charge between hearts of control and JVS.Administration of aspirin did not affect cardiac hypertrophy of JVS mice.Altogether, lipid content in diet is important for developing cardiac hypertrophy in carnitine deficiency.
2)We examined binding activity of cis-elements which exist in the region of CDV-1 promoter(-650 bp)by gel-shift analysis.The nuclear extracts prepared from JVS mice heart showed about 30% lower in cAMP responsive element, as compared with those from control.
3)We identified and characterized novel transcripts(CDV-3A and B)that may be involved in development of cardiac hypertrophy.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Jisong Peng et al.: "Identification of human CDV-1R and mouse Cdv-1R, two novel proteins with putative signal peptides, especially highly expressed in testis and increased with the male sex maturation"Mol.Biol.Rep.. 29. 353-362 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Seuta Fukumaru et al.: "Novel mRNA molecules are induced in hypertrophied ventricles of carnitine-deficient mice and belong to a family of up-regulated gene in cells overexpressing c-erbB-2"Biochim.Biophys, Acta. 1577. 437-444 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahisa Horiuchi et al.: "Improved matrix-assisted laser desorption/ionization time-of-flight mass spectrometric method for identification of amino acid o-glycosides in patients with α-N-acetylgalactosaminidase deficiency"Clin.Chem.. 48. 1798-1799 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jisong Peng, et al.: "Identification of human CDV-1R and mouse Cdv-1R, two novel proteins with putative signal peptides, especially highly expressed in testis and increased with the male sex maturation"Mol.Biol.Rep.. 29. 353-362 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Seita Fukumaru, et al.: "Novel mRNA molecules are induced in hypertrophied ventricles of carnitine-deficient mice and belong to a family of up-regulated gene in cells overexpressing c-erbB-2"Biochim.Biophys.Acta. 1577. 437-444 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahisa Horiuchi, et al.: "Improved matrix-assisted laser desorption/ionization time-of-flight mass spectrometric method for identification of amino acid o-glycosides in patients with α-N-acetylgalactosaminidase deficiency"Clin.Chem.. 48. 1798-1799 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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