2003 Fiscal Year Final Research Report Summary
Regulatory mechanisms for wound-related gene expression at invasive front of Lung adenocarcinomas
| Project/Area Number |
14570136
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
NIKI Toshihiro The University of Tokyo, Department of Human Pathology, Associate Professor, 大学院・医学系研究科, 助教授 (90198424)
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| Project Period (FY) |
2002 – 2003
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| Keywords | lung adenocarcinoma / inflammation / wound healing / invasion |
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| Research Abstract |
To elucidate the regulatory mechanisms for wound-related geneexpression at the invasive front of lung adenocarcinomas, the following investigations were performed:
(1)The promoter region of laminin-5 gamma2 gene spanning the 1.5kb region upstream of the transcription initiation site was PCR amplified and cloned into pGL3-luc vector. Preliminary experiment confirmed that this region controls the upregulation of laminin-5 gamma2 gene after stimulation with TGF-alpha. The results of this study showed that (a)This promoter activity is induced 20 fold by co-transfection of active MEK expression vector, whereas it is inhibited by pharmacologic MEK inhibitor: (2)The activity of laminin-5 gamma2 promoter is strongly repressed by the wild-type, but not by the mutant type, p53 gene. Subsequent analyses with serial deletion mutant of the laminin-5 promoters revealed that this regulatory mechanism is retained in promoters upstream of 0.5 kb transcription initiation site.
(2)To investigate the activation of wound-related signal transduction pathways in lung adenocarcinoma tissues, immunohistochemical analyses were performed using phospho-specific antibodies. The results showed that c-met is activated in 4/20 cases, erk in 15/20 cases, jnk in 3/20 cases, and p38 in 7/20 cases. As regards NF-kappaB, p65 and p50 subunits were positive in all the 20 cases examined, with certain populations of cancer cells showing nuclear immunoreactivities for these two proteins.
(3)The activation of c-met was further analyses in a total of 78cases of resected lung adenocarcinomas. The activation of the c-met receptor was detected in 14/78 cases (17.9%). Active form of c-met was localized at the surface of cancer cells, especially in adenocarcinomas of well differentiated type. Similar localized was observed in several lung adenocarcinoma cell lines.
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