• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2003 Fiscal Year Final Research Report Summary

Mechanism of myocardial tissue damage in patients with diabetes, chronic renal failure and hemodialysis

Research Project

Project/Area Number 14570171
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Human pathology
Research InstitutionFukuoka University

Principal Investigator

SAKATA Noriyuki  Fukuoka University, School of Medicine, Professor, 医学部, 教授 (20134273)

Co-Investigator(Kenkyū-buntansha) UESUGI Noriko  Fukuoka Univ., School of Medicine, Research Assistant, 医学部, 助手 (70279264)
Project Period (FY) 2002 – 2003
KeywordsChronic renal failure / Diabetes mellitus / Myocardial tissue / Myofilament / Glycoxidation / Hydroxyl radical
Research Abstract

The present study was done to clanfy the role of glycoxidation stress on the damage of cardiac muscle and arterial tissue in patients with diabetes, chronic renal failure and hemodialysis. The level of pentosidine, a glycoxidation product, was significantly increased in the matrix proteins of arterial tissues and myofilament protein of myocardium of the patients with hemodialysis and chronic renal failure as compared to controls. The calcium-binding activity arid pentosidine level of collagen, a matrix protein, was time-dependently increased with in vitro incubation of glucose. In arterial tissues, the glycoxidative modification of matrix protein may be closely related to medial calcification in patients with diabetes and chronic renal failure. The level of urea hydrogen peroxide (UHP) was significantly higher in cardiac tissues of patients with chronic renal failure and hemodialysis than in those of controls. In vitro incubation of cardiac tissues with ribose formed pentosidine in dose dependent manner of urea or serum ultrafiltrate of patients with hemodialysis. The presence of urea, dose-dependently increased the generation of UHP in these incubation systems. Blocking experiment with catalase, SOD and mannitol showed that pentosidine formation was significantly enhanced by hydroxyl radical generated from UHP via Fenton reaction. Thus, glycoxidative modification of these proteins may be enhanced by the formation of UHP via Fenton reaction and may contribute to cardiac and arterial dysfunction in patients with chronic renal failure and hemodialysis.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Yamamoto, Y.: "Possible involvement of increased glycoxidation and lipid peroxidation of elastin in atherogenesis in haemodialysis patients."Nephrol.Dial.Transplant.. 17. 630-636 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakata, N.: "Contribution of superoxide to reduced antioxidant activity of glycoxidative serum albumin."Heart Vessels. 17(1). 22-29 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Moh, A.: "Hydrogen peroxide production in diabetic myocardium : Relevance to cardiac function damage."J.Am.Coll.Cardiol.. 39. 416B (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakata, N.: "Modification of elastin by pentosidine is associated with the calcification of aortic media in patients with end-stage renal disease Nephrol."Nephrol.Dial.Transplant.. 18. 1601-1609 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakata, N.: "Calcification of the medial layer of the internal thoracic artery in diabetic patients : Relevance of glycoxidation."J.Vasc.Res.. 40(6). 567-574 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Moh, A.: "Increased production of urea hydrogen peroxide (UHP) form Maillard reaction and a UHP-Fenton pathway related to glycoxidation damage in chronic renal failure."J.Am.Soc.Nephrol.. (in print).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamamoto, Y.: "Possible involvement of increased glycoxidation and lipid peroxidation of elastin in atherogenesis in haemodialysis patients."Nephrol.Dial.Transplant.. 17. 630-636 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakata, N.: "Contribution of superoxide to reduced antioxidant activity of glycoxidative serum albumin."Heart Vessels. 17(1). 22-29 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Moh, A.: "Hydrogen peroxide production in diabetic myocardium : Relevance to cardiac function damage."J.Am.Coll.Cardiol.. 39. 416B (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakata, N.: "Modification of elastin by pentosidine is associated with the alcification of aortic media in patients with end-stage renal disease."Nephrol.Dial.Transplant. 18. 1601-1609 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakata, N.: "Calcification of the medial layer of the internal thoracic artery in diabetic patients; Relevance of glycoxidation."J.Vasc.Res.. 40(6). 567-574 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Moh, A.: "Increased production of urea hydrogen, peroxide (UHP) form Millard reaction and a UHP-Fenton pathway related to glycoxidation damage in chronic renal failure."J.Am.Nephrol.. (in print).

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 2005-04-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi