2004 Fiscal Year Final Research Report Summary
Fundamental researches to establish a therapy of neonatal TSS-like exanthematous disease
| Project/Area Number |
14570246
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Tokyo Women's Medical University |
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Principal Investigator |
KATO Hidehito Tokyo Women's Medical University, School of Medicine, Instructor, 医学部, 助手 (00241084)
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| Co-Investigator(Kenkyū-buntansha) |
UCHIYAMA Takehiko Tokyo Women's Medical University, School of Medicine, Professor, 医学部, 教授 (00050550)
IMANISHI Ken'ichi Tokyo Women's Medical University, School of Medicine, Assistant Professor, 医学部, 助教授 (20132920)
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| Project Period (FY) |
2002 – 2004
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| Keywords | MRSA / Neonatal TSS-like Exanthematous Disease / Super Antigen |
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| Research Abstract |
Bacterial superantigens(SAGs) activate a vast number of T cells in a T cell receptor(TCR) V□-selective manner. Staphylococcus aureus produces a variety of SAGs, such as toxic shock syndrome toxin-1(TSST-1), and staphylococcal enterotoxins A to M (SEA-SEM). It is well known that TSST-1 causes toxic shock syndrome(TSS) and neonatal TSS-like exanthematous disease(NTED), and that SEA-SEE cause food-poisoning. Recently genes (set1-set5) encoding a number of proteins similar to staphylococcal SAGs were found in one Staphylococcus strain (R.J.Williams et al.Infect.Immun.68:4407). Recently genes set6-set15 have been identified in other strain of this bacterium (M.Kuroda et al.Lancet 357:1225,2001). We analysed immunobiological properties of the products of these genes, SET6-SET15.
Although all the SET proteins are similar in amino acid sequence, they can be divided into several groups on phylogenetic analysis : Group 1 (SET-6 and SET-15), Group 2 (SET-7,SET-8,and SET-9), and Group 3 (SET-12 and SET-13). SET-10,SET-11,and SET-14 are unique.
We prepared recombinant SETs (r-SETs) as fusion proteins of GST. rSETs were analysed after removal of GST. SETs activated human T cells to produce IL-2 and IFN-γ in the presence of HLA-DR+L cells. To examine the TCR Vβ repertoire of SET-reactive human T cells, peripheral blood T cells were stimulated with different SETs and the T cell blasts collected were expanded with human IL-2.
We found that SET did not activate T cells. Therefore, we concluded that SET did not affect the onset of TSS.
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[Journal Article] A co-stimulatory molecule on activated T cells, H4/ICOS, delivers specific signals in Th cells and regulates their responses.2002
Author(s)
Arimura Yutaka, Kato Hidehito, Dianzani Umberto, Okamoto Toshihiro, Kamekura Soichiro, Buonfiglio Donatella, Miyoshi-Akiyama Toru, Uchiyama Takehiko, Yagi Junji
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Journal Title
Int Immunol 14(6)
Pages: 555-566
Description
「研究成果報告書概要(欧文)」より
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