2003 Fiscal Year Final Research Report Summary
Identification of host factor interacting with IpaC effector molecules of Shigella sonnei and its physiological function
| Project/Area Number |
14570257
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
WATANABE Haruo National Institute of Infectious Diseases, Department of Bacteriology, Director, 細菌第一部, 部長 (70142130)
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| Co-Investigator(Kenkyū-buntansha) |
HIROSE Kenji National Institute of Infectious Diseases, Department of Bacteriology, Chief, 細菌第一部, 主任研究員 (70311397)
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| Project Period (FY) |
2002 – 2003
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| Keywords | Shigella / cell invasion / phagocytosis / IpaC / effector molecule / actin / β-catenin |
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| Research Abstract |
IpaC of Shigella is essential for initial bacterial entry into epithelial cells. We report that IpaC interacts with β-catenin and destabilizes the cadherin-mediated cell adhesion complex. Using a yeast two-hybrid system, we identified β-catenin as a binding partner of IpaC within the host cell after cell entry, but not in the initial entry. Co-immunoprecipitation, confocal microscopy, and GST pull down experiments confirmed the intracellular and cell-free interactions between these two proteins. The interaction sites were mapped to the ninth armadillo repeat of β-catenin and to the C-terminus of IpaC. IpaC-associated β-catenin was phosphorylated at tyrosine residues. This phosphorylation led to the destabilization of the functional cadherin-catenin complex, which could be a mechanism whereby the epithelial cell-cell tight adhesion is disrupted. These events may facilitate the further basolateral invasion of bacteria through the disrupted space and/or modulate the cell-to-cell spread of Shigella.
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[Publications] Hirose, K., Itoh, K., Nakajima, H., Kurazono, T., Moriya, K., Ezaki, T., Tamura, K., Watanabe, H.: "Selective amplication of rfbE, rfbS and fliC by multiplrx PCR for identification and detection for Salmonella enetrica serovar Typhi and Paratyphi A."J. Clinic Microbial.. 40. 633-636 (2002)
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[Publications] Nakaya, H., Yasuhara, A., Yoshimura, K., Oshihoi, Y., Izumiya, H., Watanabe, H.: "Life-threatening infantile diarrhea from fluoroquinolone -resistant Salmonella enterica Typhimurium with mutations in both gyrA and parC."Emerg.Infect.Dises.. 9. 255-257 (2003)
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[Publications] Hirose, K., Itoh, K., Arawawa, A., Tamura, K., Watanabe, H.: "DNA-based diagnosis method for typhoid and paratyphoid fever, and the screening method for Salmonella enterica serovar Typhi and serovar Paratyphi A with decreased susceptibility to fluoroquinolones by PCR-restriction fragment length polymorphism (RFLP)."Res.Adv. in Microbiology. 3. 109-121 (2003)
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[Publications] Taniya T., Mitobe, J., Nakayama, S., Qi, M-S., Okuda, K., Watanabe, H.: "Determination of InvE binding site required for the expression of IpaB in Shigella sonnei : involvement of PrB BoxA-like sequence."J.Bacteriol.. 185. 5158-5165 (2003)
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[Publications] Nakayama, S., Kushiro, A., Asahara, T., Tanaka, R., Hu, l., Kopecko, D.J., Watanabe, H.: "Activation of hi1A and invasion genes at low pH requires cpxA but not the putative cognate response regulator cpxR in Salmonella enterica serovar Typhimrium."Microbiology. 149. 2809-2817 (2003)
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