2003 Fiscal Year Final Research Report Summary
Analysis of human herpesvirus 6 latent infection in the brain.
| Project/Area Number |
14570263
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | The Jikei University School of Medicine (2003)
Osaka University (2002) |
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Principal Investigator |
KONDO Kazuhiro The Jikei University School of Medicine, Professor, 医学部, 教授 (70234929)
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| Co-Investigator(Kenkyū-buntansha) |
YAMANISHI Koichi Osaka University Medical School, Professor, 医学系研究科, 教授 (10029811)
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| Project Period (FY) |
2002 – 2003
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| Keywords | human herpesvirus 6 / HHV-6 / latency / latency-associated transcript / reactivation / immediate early gene / Heterogeneous Ribonucleoprotein K / Casein Kinase 2 |
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| Research Abstract |
Four kinds of latency-associated transcripts of human herpesvirus 6 were identified which were detected only in latently infected cells. Although they were oriented in the same direction as the immediate-early land 2 (IE1/IE2) genes and shared their protein-coding region with IE1/IE2, their transcription start sites and exon(s) were latency associated.
Latency-associated transcripts of human herpesvirus 6 (H6LTs) (K.Kondo et al.J.Virol.76:4145-4151, 2002) were maximally expressed at a fairly stable intermediate stage between latency and reactivation both in vivo and in vitro. H6LTs functioned as sources of immediate-early protein 1 at this stage, which up-regulated the viral reactivation.
The U3-U7 gene cluster of human herpesvirus 6 (HHV-6) was replaced with an enhanced green fluorescent protein-puromycin gene cassette containing the cytomegalovirus major immediate-early promoter. Neither viral replication in T cells nor latency and reactivation in macrophages was impaired. During HHV-6 latency, the cytomegalovirus promoter used the transcription start sites employed in cytomegalovirus latency.
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