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2003 Fiscal Year Final Research Report Summary

STUDY ON INFECTIOUS ROUTE OF HERPES SIMPLEX VIRUS FROM MUCOSA TO NERVOUS TISSUES

Research Project

Project/Area Number 14570265
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionNagasaki University

Principal Investigator

IWASAKI Takuya  Nagasaki University, Institute of Tropical Medicine, Professor, 熱帯医学研究所, 教授 (90146027)

Co-Investigator(Kenkyū-buntansha) SATA Tetsutaro  National Institute of Infectious Diseases, Director, 感染病理部, 部長 (00162397)
Project Period (FY) 2002 – 2003
Keywordshelpes simplex virus / central nervous tissue / mucosal infection / pathogenesis / Schwann cells / immediate early protein
Research Abstract

Herpes simplex virus (HSV) initially infects the, squamous cell epithelium of mucosa and skin, then, invade the peripheral nervous fibers in the subepithelial tissues at primary infection. Thereafter, HSV ascends through the nerve fibers to the soma of neurons in the central nervous system (CNS). In CNS HSV becomes to fall in a latent condition by host immune response. In this study we focused on the route of HSV infection from the squamous cell epithelium to the nervous fibers, in comparison with that of poliovirus because of similar usage of virus receptors.
Various strains of HSV-2 including US2-deleted and its revertant recombinants, were inoculated on the corneal and vaginal surface of BALB/c mice. These mice were examined for their manifestations and histopathological changes. In addition, the. expression of immediate early protein (US1) was also investigated with respect to epithelial, subepithelial and nervous stages of viral infection and host immune responses.
By immunohistochemical detection of US1 expression in formalin-fixed paraffin sections of HSV-2 inoculated mice, we have succeeded to orient the early infected cells, in which US1 antigen was detected in their nuclei. From this analysis we could demonstrate HSV-2 infection, in Schwann cells and satellite cells around the ganglion cells. In addition, the infection in Schwann cells without marked cellular destruction in the posterior column in the white matter of spinal cord is important to consider the pathogenesis of HSV-2 meningitis. Further study, we can reveal the difference of HSV-1 and HSV-2 after mucosal infection.

  • Research Products

    (4 results)

All Other

All Publications (4 results)

  • [Publications] 峰咲幸哲, 本田まりこ, 岩崎琢也, 白木公康: "BALB/cマウスへの単純ヘルペスウイルス2型臨床分離株経膣接種による神経伝達経路と脊髄炎の発症病理"東京慈恵会医科大学雑誌. 117・6. 427-439 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagata N, Iwasaki T, Ami Y, Sato Y, Hatano I, Harashima A, Suzaki Y, Yoshii T, Hashikawa T, Sata T, Horiuchi Y, Koike S, Kurata T, Nomoto A.: "A poliomyelitis model through mucosal infection in transgenic mice bearing human poliovirus receptor, TgPVR21."Virology. 321-1. 87-100 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshinori Minesaki, Mariko Honda, Takuya Iwasaki, Kimiyasu Shiraki: "Pathogenesis of the myelitis and the transneuronal spread of herpes simplex virus type 2 after intravaginal inoculation into BALB/c mice."Tokyo Jikeikai Medical Jornal. 117-6. 427-439 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagata N, Iwasaki T, Ami Y, Sato Y, Hatano I, Harashima A, Suzaki Y, Yoshii T, Hashikawa T, Sata T, Horiuchi Y, Koike S, Kurata T, Nomoto A.: "A poliomyelitis model through mucosal infection in transgenic mice bearing human poliovirus receptor, TgPVR21."Virology. 321-1. 87-100 (2004)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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