2003 Fiscal Year Final Research Report Summary
Analysis of human NKT cells and the development of exvivo expansion methods
| Project/Area Number |
14570291
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | National Cancer Center Research Institute |
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Principal Investigator |
WAKASUGI Hiro NCCRI, Pharmacology Division, Chief, 薬効試験部, 部長 (10112632)
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| Co-Investigator(Kenkyū-buntansha) |
IKARASHI Yoshinori NCCRI, Pharmacology Division, Research Associate, 薬効試験部, 研究員 (70280782)
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| Project Period (FY) |
2002 – 2003
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| Keywords | V_α24^+NKT / α-GalCer / G-CSF / IL-2 / IL-4 / CD1d非拘束性細胞障害 |
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| Research Abstract |
Efficient expansion of V_α24^+NKT (V_α24^+CD3^+) cells from granulocyte colony-stimulating factor(G-CSF)-mobilized peripheral blood(PB) cells is possible under culture conditions with α-GalCer, IL-2 and fetal bovine serum(FBS) in vitro^6. In an attempt to avoid potential risks associated with FBS and to establish a clinical-scale expansion system, we investigated the development of a more efficient non-FBS expansion system for V_α24^+ NKT cells with α-GalCer and IL-2.
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[Journal Article] The mouse natural killer T cell-associated antigen recognaized by U5A2-13 monoclonal antibody is intercellular adhesion molecule-12004
Author(s)
Shimizu, A., Sasaki, H., Aoyagi., Yoshida, M., Kato, K., Heike, Y., Ikarashi, Y., Shirakawa, K., Takaue, Y., Miyajima, A., Terada, M., Nagai, H., Wakasugi, H.
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Journal Title
Immunology Letters 92
Pages: 227-235
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] IL-4 Confers NK Stimulatory Capacity to Murine Dendritic Cells : A Signaling Pathway Involving KARAP/DAP12-Triggering Receptor Express on Myeloid Cell 2 Molecules^12004
Author(s)
Terme M, Tomasello E, Maruyama K, Crepineau F, Chaput N, FlamentC, Marolleau JP, Angevin E, Wagner EF, Salomon B, Lemonnier FA, Wakasugi H, Colonna M, Vivier E, Zitvogel L.
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Journal Title
J.Immunol. 172(10)
Pages: 5957-5966
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Novel mode of action of c-kit tyrosine kinase inhibitors leading to NK cell-dependent antitumor effects.2004
Author(s)
Borg C, Terme M, Taieb J, Menard C, Flament C, Robert C, Maruyama K, Wakasugi H, Angevin E, Thielemans K, Le Cesne A, Chung-Scott V, Lazar V, Tchou I, Crepineau F, Lemoine F, Bernard J, Fletcher JA, Turhan A, Blay JY, Spatz A, Emile JF, Heinrich MC, Mecheri S, Tursz T, Zitvogel L.
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Journal Title
J Clin Invest. 114(3)
Pages: 379-388
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] NK cell activation by Dendritic Cells (DC)require The Formation of a Synapse leading to IL-12 Polarization in DC.2004
Author(s)
Borg C, Terme M, Taieb J, Menard C, Flament C, Robert C, Maruyama K, Wakasugi H, Angevin E, Thielemans K, Le Cesne A, Chung-Scott V, Lazar V, Tchou I, Crepineau F, Lemoine F, Bernard J, Fletcher JA, Turhan A, Blay JY, Spatz A, Emile JF, Heinrich MC, Mecheri S, Tursz T, Zitvogel L.
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Journal Title
J Clin Invest. 114(3)
Pages: 379-388
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Role Of Natural Killer T Cells In Anti-Tumor Immune Response.2004
Author(s)
Yoshinori Ikarashi, Yuji Heike, Atsushi Shimizu, Osamu Imataki, Sachiyo Kanai, Mitsuzi Yoshida, Akira liduka, Takayuki Morikawa, Yoichi Takauel, Hiro Wakasugi.
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Journal Title
Extended Abstracts for the 34nd International Symposium of the Princess Takamatsu Cancer Research Fund, Tokyo, 2004.
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] U5A2-13, an antigen originally found on mouse NK-like T Cells, is an early inducible cell surface antigen during lymphoid activation.2003
Author(s)
Kazunori Kato, Yoshinori Ikarashi, Toshiaki Sugahara, Atsushi Yasumoto, David Sancho, Mitsuzi Yoshida, Yoichi Takaue, Yoshiro Kobayashi, Francisco Sanchez-Madrid, Hiro Wakasugi.
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Journal Title
Cellular Immunology 221
Pages: 27-36
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] CD4^+ CD25^+ T cells responding to serologically defined autoantigens suppress antitumor immune responses.2003
Author(s)
Hiroyoshi Nishikawa, Takuma Kato, Koji Tanida, Atsunori Hiasa, Isao Tawara, Hiroaki Ikeda, Yoshinori Ikarashi, Hiro Wakasugi, Mitchell Kronenberg, Toshinori Nakayama, Masaru Taniguchi, Kagemasa Kuribayashi, Lloyd J.Old, Hiroshi Shiku.
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Journal Title
Proc Natl Acad Sci USA 100
Pages: 10902-10906
Description
「研究成果報告書概要(欧文)」より
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