2003 Fiscal Year Final Research Report Summary
Study on Regulatory Mechanisms of Orexin Neuronal Activity that regulate Emotion and Stress Responses
| Project/Area Number |
14570296
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hygiene
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| Research Institution | University of Tsukuba |
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Principal Investigator |
MATSUZAKI Ichiyo University of Tsukuba, Community Medicine, Associate Professor, 社会医学系, 助教授 (10229453)
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| Co-Investigator(Kenkyū-buntansha) |
SAKURAI Takeshi University of Tsukuba, Basic Medical Sciences, Associate Professor, 基礎医学系, 助教授 (60251055)
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| Project Period (FY) |
2002 – 2003
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| Keywords | stress / neuropeptide / transgenic / hypothalamus / emotion / serotonin / orexin / EGFP |
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| Research Abstract |
We generated transgenic mouse lines, in which orexin-producing neurons exclusively express the green fluorescent protein (GFP), using human prepro-orexin promoter. We prepared fluorescing neurons from the hypothalamus of these mice, and subjected them to whole cell patch-clamp analysis. By this system, we identified several factors that have effects on orexin neuronal activity ; CRF, ghrelin, CCK, and actylcholine activated, while noradrenalin and serotonin inhibited the activity of orexin neuron. Because monoamines, such as noradrenalin and serotonin have a role in regulation of emotion, while CRF and CCK are important factors for the stress response, these results suggest a possibility that orexin has an important role in the regulation and emergence of the emotional behavior and stress response.
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