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2003 Fiscal Year Final Research Report Summary

The association of the gene polymorphism of APRIL and BAFF with autoimmune diseases : Functional analysis

Research Project

Project/Area Number 14570415
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

TSUKAINOTO Hiroshi  KYUSHU UNIVERSITY, University Hospital, Research Associate, 大学病院, 助手 (70304772)

Co-Investigator(Kenkyū-buntansha) HORIUCHI Takahiko  KYUSHU UNIVERSITY, University Hospital, Assistant Professor, 大学病院, 講師 (90219212)
Project Period (FY) 2002 – 2003
KeywordsSystemic lupus erythematosus / Rheumatoid arthritis / genetic analysis / single nucleotide polymorphisims / TNF family / cytokine
Research Abstract

Objective. To investigate the association of the gene polymorphisms in APRIL and BAFF, new members of the TNF family, with systemic lupus erythematosus(SLE)
Results. The human APRIL gene comprised at least six exons with five introns spanning approximately 2.4 kb of the genomic DNA. By exon-specific PCR/SSCP, we identified two novel polymorphisms at codons 67 and 96 Both had amino acid substitutions, G67R and N96S, respectively. Only the 67G allele was associated with SLE in 148 Japanese SLE patients with allele frequency 0.662 compared with 0.575 for 146 healthy controls (p=0.0302) The frequency of the individuals who possessed at least one 67G allele in SLE patients (91.9%) was significantly higher than that in normal controls (80.1%) (p=0.0036). There were no associations between the polymorphisms in BAFF gene and SLE. We developed ELISA for APRIL and BAFF, and demonstrated that serum levels of APRIL and BAFF were elevated in patients with SLE compared with healthy controls.
Conclusion. These results suggest that the 67G allele of APRIL is a contributing factor in the pathogenesis of SLE. The elevated serum levels of APRIL and BAFF may be associated with the pathogenesis of SLE.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Harashima S, Tsukamoto H, Horiuchi T: "Osteoprotegerin and receptor activator of nuclear facter-κB ligand expression in fibroblast-like synoviocytes from rheumatoid arthritis and osteoarthritis patients."Rheumatology. 43. 396-397 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koyama T, Tsukamoto H, Masumoto K et al.: "A novel polymorphism of the human APRIL gene is associated with systemic lupus erythematosus"Rheumatology. 42. 980-985 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sawabe T, Horiuchi T, Koga R, Tsukamoto H, et al.: "Aberant HS1 molecule in patient with systemic lupus erythematosus"Genes and Immunity. 4. 122-131 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsukamoto H, Tousson A, Circolo A, et al.: "Calnexin is associated and induced by overexpressed human complement protein C2."Anat Rec. 267. 7-16 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Himeji D, Horiuchi T, Tsukamoto H, et al.: "Characterization of caspase-8L : a novel isoform of caspase-8 that behaves as an inhibitor of the caspase cascade."Blood. 99. 4070-4078 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Harashima S, Tsukamoto H, Nishizaka H, Otsuka J, Horiuchi T: "Successful treatment of invasive pulmonary aspergillosis by transbronchial injection of urinary trypain inhibitor and amphotericin B"Acta Haematol. 109. 156-157 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Horiuchi T, Nishimukai H, Okiura T, Nishimura K, Nishizaka H, Kojima T, Tsukamoto H, Hayashi K, Hrada M: "Molecular bases for human complement C7 polymorphysms, C7^*3 and C7^*4"Biochem Biophys Res Commun. 298. 450-455 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Harashima S, Tsukamoto H, Horiuchi T: "Osteoprotegerin and receptor activator of nuclear factor κB ligand expression in fibroblast-like synoviocytes from rheumatoid arthritis and osteoarthritis patients."Rheumatology. 43. 296-397 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koyama F.Tsukamoto H, Masumoto K, Himeji D, Havashi K, Harada M, Horiuchi T.: "A novel polymorphism of the human APRIL gene is associated with systemic lupus erythematosus."Rheunmtology. 42. 980-985 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Harashima S, Tsukamoto H, Nishizaka H, Otsuka J, Horiuchi T: "Successful treatment of invasive pulmonary aspergillosis by transbronchial injection of urinary trypsin inhibitor and amphotericin B."Acta Haematol. 109. 156-157 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sawabe F, Horiuchi T, Koga R, Tsukamoto H, Kojima T, Harashima S, Kikuchi Y, Otsuka J, Mitoma H, Yoshizawa S, Niho Y, Watanabe T: "Aberrant HSI molecule in a patient with systemic lupus erythematosus."Genes and Immunity. 4. 122-131 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Horiuchi F, Nishimukai H, Okiura T, Nishimura K, Nishizaka H, Kojima T, Tsukamoto H, Hayashi K, Harada M: "Molecular bases for human complement C7 polymorphysms, C7^*3 and C7^*4."Biochem Biophys Res Commun. 298. 450-455 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsukamoto H, Tousson A, Circolo A, Marchase RB. Volanakis JE: "Calnexin is associated and nduced by overexpressed human complement prtein C2."Anat Rec. 267. 7-16 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Himeji D.Horiuchi T, Tsukamoto H, Hayashi K, Watanabe T, Harada M: "Characterization of caspase-8L : a novel isoform of caspase-8 that behaves as an inhibitor of the caspase cascade,"Blood. 99. 4070-4078 (2002)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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