2003 Fiscal Year Final Research Report Summary
Transdifferentiation of bone marrow-derived cells into hepatic lineage cells: the methods of transdifferentiation in vitro and hepatic cells transplantation in vivo
| Project/Area Number |
14570467
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | OSAKA UNIVERSITY |
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Principal Investigator |
KISO Shinichi Osaka University Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (40335352)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUI Kouji Osaka University Graduate School of Medicine, Medical Staff, 医学部附属病院, 医員(臨床研究)
IGURA Takumi Osaka University Graduate School of Medicine, Medical Staff, 医学部附属病院, 医員(臨床研究)
TAMURA Shinji Osaka University Graduate School of Medicine, Assistant Professor, 医学系研究科, 助手 (30243223)
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| Project Period (FY) |
2002 – 2003
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| Keywords | Bone marrow cells / hepatocytes / trans-differentiation / bFGF / HGF / 再生医療 |
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| Research Abstract |
Evidence that bone marrow cells have trans-differencing potential to hepatocytes have been described in recent reports. However, the molecular mechanism underlying this phenomenon is unclear. To address this issue, we investigated the parameters involved in the trans-differentiation ob bone marrow cells into hepatic lineage. (Methods) Mouse BM cells were cultured in a collagen gel without or with growth factors, including basic fibroblast growth factor. The expression of hepatocyte-specific markers, cholangiocyte-specific marker and liver-enriched transcription factors was identified by RT-PCR and immunohistochemistry. (Results) Basic fibroblast growth factor was found to be the most effective for inducing albumin in cultured BM cells. Furthermore, on stimulation of basic fibroblast growth factor, BM cells were found to express other hepatocyte-specific markers and a cholangiocyte-specific marker. This conversion was found to be associated with the induction of transcription factors including hepatocyte nuclear factors and GATA family proteins. (Conclusion) We established an in vitro culture system in which mouse bone marrow cells could trans-differentiate to hepatic lineage cells in response to growth factors. In particular, basic fibroblast growth factor has the ability to induce the trans-differentiation into hepatic lineage cells from BM cells.
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Research Products
(13 results)
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[Publications] Shinichi Kiso, Sumio Kawata, Shinji Tarnura, Yoshiaki Inul, Yuichi Yoshida, Yoshiyuki Sawai, Shinobu Umeki, Nobuyuki Ito, Akira Yamada, Jun-ichiro Miyagawa, Shigeki Higashiyama, Takao Iwawaki, Michiko Saito, Naoyuki Taniguchi, Yuji Matsuzawa, Kenji Kohno.: "Liver Regeneration in Heparin-Binding EGF-like Growth Factor Transgenic Mice after Partial Hepatectomy."Gastroenterology. 124. 701-707 (2003)
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「研究成果報告書概要(欧文)」より
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[Publications] Yoshihiro Kamada, Shinji Tamura, Shinichi Kiso, Hitoshi Matsumoto, Yukiko Saji, Yuichi Yoshida, Koji Fukui, Norikazu Maeda, Hitoshi Nishizawa, Hiroyuki Nagaretanl, Yoshihiro Okamoto, Shinji Kihara, Jun-ichiro Miyagawa, Yasuhisa Shinomura, Tohru Funahashi, Yuji Matauzawa: "Enhanced carbon tetrachloride-induced liver fibrosis in mice lacking adiponectin."Gastroenterology. 125. 196-1807 (2003)
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[Publications] Yashihiro Kamada, Shinji Tamura, Shinichi Kiso, Koji Fukui, Yoshinori Doi, Nobuyuki Ito, Yuki Terui, Takafumi Saito, Hisayoshi Watanabe, Hitoshi Togashi, Sumio Kawata, Yuji Matsuzawa: "Anglotensin II stimulates the nuclear translocation of Smad2 and induces PAl-1 mRNA In rat hepatic stellate cells."Hepatology Research. 25. 296-305 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Shigeru Sakuda, Shinji Tamura, Akira Yamada, Jun-ichiro Miyagawa, Koji Yamamoto, Shinichi Kiso, Nobuyuki Ito, Shigeki Higashiyama, Naoyuki Taniguchi, Sumio Kawata, Yuji Matsuzawa.: "NF-kB activation in non-parenchymal liver cells after partial hepatectomy in rats: possible involvement in expression of heparin-binding epidermal growth factor-like growth factor."J Hepatology. 36. 527-533 (2002)
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「研究成果報告書概要(欧文)」より
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[Publications] Shigeru Sakuda, Shiji Tamura, Akira Yamada, Jun-ichiro Miyagawa, Koji Yamamoto, Shinichi Kiso, Nobuyuki Ito, Kazuho Imanaka, Akira Wada, Tetsuji Naka, Tadamitsu Kishimoto, Sumio Kawata, Yuji Matsuzawa.: "Activation of signal transducer and activator transcription 3 nd expression of suppressor of cytokine signal 1 during liver regeneration in rats."J Hepatology. 36. 378-384 (2002)
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「研究成果報告書概要(欧文)」より
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