2002 Fiscal Year Annual Research Report

心肥大・血管新生の機序解明と治療的電気刺激血管新生法の確立と応用

Research Project

Project/Area Number	14570634
Research Institution	Tohoku University
Principal Investigator	上月正博東北大学, 大学院・医学系研究科, 教授 (70234698)
Co-Investigator(Kenkyū-buntansha)	小野寺宏東北大学, 大学院・医学系研究科, 助教授 (20214207) 佐藤寿伸東北大学, 医学部・附属病院, 助教授 (50312583) 金澤雅之東北大学, 大学院・医学系研究科, 助教授 (60282050) 半田康延東北大学, 未来科学技術共同研究センター, 教授 (00111790) 加賀谷豊東北大学, 医学部・附属病院, 講師 (90250779)
Keywords	VEGF / HGF / low voltage electrical stimulation / ischemic muscle
Research Abstract	ラットの両側大腿動脈を切離し虚血肢モデルを作成した。一週間の回復期間を与えた後、電極(陰極)を両側前脛骨筋中央の筋肉内に留置し、肩甲骨下より出した。右側の前脛骨筋に留置した電極をパルスジェネレーターに接続し電気刺激肢とした。左側の前脛骨筋に留置した電極をパルスジェネレーターに接続せず非刺激肢とした。 1)5日間刺激の血管新生因子の効果:continuous LVES(CL-VES)、intermittent HVES(IH-VES)の2条件間の比較. 筋収縮閾値以下の電気刺激(CL-VES)が虚血骨格筋の血管新生因子であるVEGF、HGF、FGF、あるいは炎症・低酸素因子であるIL6、HIFに及ぼす影響について検討した。さらに収縮性電気刺激(IH-VES)との比較を行った。CL-VESではHGF、VEGFの増加を認めたが、IL6、HIF、FGFに関しては差異を認めなかった。IH-VESではVEGFの増加を認めたがHGFの増加を認めなかった。虚血肢に対するCL-VESは、低酸素や炎症を介さずにVEGF・HGFを増加させることにより血管新生に関与し,その機序はIH-VESとは異なる可熊性が示唆された。 2)1日間刺激の血管新生因子の効果:CL-VESとIH-VESの比較 CL-VES群において、電気刺激肢のVEGF蛋白量は非刺激肢に比較し有意に増加した。一方、IH-VES群において、電気刺激肢のVEGF蛋白量は非刺激肢と比較して差異を認めなかった。すなわち、1日間のラット骨格筋に対する電気刺激ではCL-VES群においてのみ非刺激肢と比べてVEGF蛋白量の統計学的有意差を認めた。一方、1日間刺激に対する5日間刺激のVEGF蛋白量/コントロール群のVEGF蛋白量の比率の増加はIH-VES群においてのみ認められ、CL-VES群においては認められなかった。

Research Products

(13 results)

All Other

All Publications (13 results)

[Publications] Kanazawa M: "Combination therapy with an angiotensin-converting enzyme(ACE)inhibitor and a calcium antagonist : beyond the renoprotective effects of ACE inhibitor monotherapy in a spontaneous hypertensive rat with renal ablation"Hypertens Res. 25. 447-453 (2002)
[Publications] Chida K: "Ventriculography using ECG-gated multiple diastolic injection of contrast material in pediatric angiocardiography"Pediatr Cardiol. 23. 200-204 (2002)
[Publications] Minami N: "Effect of estrogen on pressor responses to α1-Adrenoreceptor agonist in conscious female rats"Hypertens Res. 25. 609-613 (2002)
[Publications] Kawamura T: "Impact of exercise and ACE inhibition on TNF-α and leptin in hypertensive rats"Hypertens Res. 25. 919-926 (2002)
[Publications] Yoshida K: "Chronic angiotensin-converting enzyme inhibition and angiotensin II antagonism in rats with chronic renal failure"J Cardiovasc Pharmacol. 40. 533-542 (2002)
[Publications] Chida K: "Stability of electron-beam energy monitor for quality assurance of the electron-beam energy from radiotherapy accelerators"Tohoku J Exp Med. 198. 197-201 (2002)
[Publications] Mori N: "Altered ventilatory patterns and efficacies in patients with severe cerebral palsy with spinal rotation, scoliosis, and mental retardation"Proceedings of the 2nd World Congress of the International Society of Physical and Rehabilitation Medicine, Monduzzi Editore, Bologna. (in press). (2003)
[Publications] Matsumoto K: "Reduced lung volume after chest physiotherapy"Proceedings of the 2nd World Congress of the International Society of Physical and Rehabilitation Medicine, Monduzzi Editore, Bologna. (in press). (2003)
[Publications] Ji L: "Disability prevention of renal failure : Effects of exercise and enalapril in Thy-l nephritis rats"Proceedings of the 2nd World Congress of the International Society of Physical and Rehabilitation Medicine, Monduzzi Editore, Bologna. (in press). (2003)
[Publications] Kataoka H: "Generic & disease-specific quality of life, anxiety and depression in Japanese colostomates"Proceedings of the 2nd World Congress of the International Society of Physical and Rehabilitation Medicine, Monduzzi Editore, Bologna. (in press). (2003)
[Publications] Goto Y: "Long-term effects of lung volume reduction in exercise capacity, activities of daily living (ADL) and quality of life"Proceedings of the 2nd World Congress of the International Society of Physical and Rehabilitation Medicine, Monduzzi Editore, Bologna. (in press). (2003)
[Publications] Kohzuki M: "Disability prevention of renal failure : effects of exercise and enalapril in nephritic rats"Proceedings of the 2nd World Congress of the International Society of Physical and Rehabilitation Medicine, Monduzzi Editore, Bologna. (in press). (2003)
[Publications] 上月正博: "運動障害のリハビリテーション"南江堂. 275 (2002)

2002 Fiscal Year Annual Research Report

心肥大・血管新生の機序解明と治療的電気刺激血管新生法の確立と応用

Principal Investigator

上月 正博 東北大学, 大学院・医学系研究科, 教授 (70234698)

Research Products

[Publications] Kanazawa M: "Combination therapy with an angiotensin-converting enzyme(ACE)inhibitor and a calcium antagonist : beyond the renoprotective effects of ACE inhibitor monotherapy in a spontaneous hypertensive rat with renal ablation"Hypertens Res. 25. 447-453 (2002)

[Publications] Chida K: "Ventriculography using ECG-gated multiple diastolic injection of contrast material in pediatric angiocardiography"Pediatr Cardiol. 23. 200-204 (2002)

[Publications] Minami N: "Effect of estrogen on pressor responses to α1-Adrenoreceptor agonist in conscious female rats"Hypertens Res. 25. 609-613 (2002)

[Publications] Kawamura T: "Impact of exercise and ACE inhibition on TNF-α and leptin in hypertensive rats"Hypertens Res. 25. 919-926 (2002)

[Publications] Yoshida K: "Chronic angiotensin-converting enzyme inhibition and angiotensin II antagonism in rats with chronic renal failure"J Cardiovasc Pharmacol. 40. 533-542 (2002)

[Publications] Chida K: "Stability of electron-beam energy monitor for quality assurance of the electron-beam energy from radiotherapy accelerators"Tohoku J Exp Med. 198. 197-201 (2002)

[Publications] Matsumoto K: "Reduced lung volume after chest physiotherapy"Proceedings of the 2nd World Congress of the International Society of Physical and Rehabilitation Medicine, Monduzzi Editore, Bologna. (in press). (2003)

[Publications] Ji L: "Disability prevention of renal failure : Effects of exercise and enalapril in Thy-l nephritis rats"Proceedings of the 2nd World Congress of the International Society of Physical and Rehabilitation Medicine, Monduzzi Editore, Bologna. (in press). (2003)

[Publications] Kataoka H: "Generic & disease-specific quality of life, anxiety and depression in Japanese colostomates"Proceedings of the 2nd World Congress of the International Society of Physical and Rehabilitation Medicine, Monduzzi Editore, Bologna. (in press). (2003)

[Publications] Goto Y: "Long-term effects of lung volume reduction in exercise capacity, activities of daily living (ADL) and quality of life"Proceedings of the 2nd World Congress of the International Society of Physical and Rehabilitation Medicine, Monduzzi Editore, Bologna. (in press). (2003)

[Publications] Kohzuki M: "Disability prevention of renal failure : effects of exercise and enalapril in nephritic rats"Proceedings of the 2nd World Congress of the International Society of Physical and Rehabilitation Medicine, Monduzzi Editore, Bologna. (in press). (2003)

[Publications] 上月正博: "運動障害のリハビリテーション"南江堂. 275 (2002)

上月正博東北大学, 大学院・医学系研究科, 教授 (70234698)