2003 Fiscal Year Final Research Report Summary
Mechanisms of exacerbation of asthma induced by RS virus infection and it's regulation due to drugs
| Project/Area Number |
14570723
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Pediatrics
|
|---|
| Research Institution | Gunma University |
|---|
Principal Investigator |
MORIKAWA Akihiro Gunma University Graduate School, Pediatrics & Developmental Medicine, Professor, 医学部, 教授 (40125878)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MOCHIZUKI Hiroyuki Gunma University, Medicine, Lecturer, 医学部, 講師 (50270856)
|
|---|
| Project Period (FY) |
2002 – 2003
|
| Keywords | RSV / eosinophil / oxygen radical / degranulation / airway epithelium / bronchial asthma / airway inflammation / Mac-1 |
|---|
| Research Abstract |
Human respiratory syncytial virus (RSV) infection in infancy and early childhood causes acute bronchiolitis and exacerbates bronchial asthma. Eosinophil infiltration may contribute to airway obstruction in RSV infection. We hypothesized that RSV affects eosinophil function. Eosinophil activation was evaluated by chemiluminescent detection of superoxide anion (O(2)(-)) generation. Expression of CD11b on eosinophils was determined by flow cytometry. Although RSV did not induce O(2)(-) generation by resting eosinophils, RSV enhanced O(2)(-) generation of eosinophils primed with platelet-activating factor (PAF). Enhancement was significantly inhibited by either continuous agitation to prevent eosinophil adhesion to test tube surfaces or by pretreating cells with anti-CD18 antibody, suggesting that the stimulatory effects of RSV on eosinophils depend on cell adhesion via beta(2)-integrins. In fact, RSV enhanced PAF-induced CD11b expression by eosinophils. These findings suggest that RSV enhances eosinophil CD11b expression and O(2)(-) generation induced by PAF. Thus, RSV infection may exacerbate airway inflammation by enhancing mediator release from eosinophils.
|
Research Products
(18 results)
-
-
-
-
-
-
-
-
-
[Publications] Takizawa T, Kato M, Suzuki M, Tachibana A, Motegi Y, Fujiu T, Kimura H, Arakawa H, Mochizuki H, Tokuyama K, Morikawa A.: "Distinct isoforms of protein kinase C are involved in human eosinophil functions induced by platelet-activating factor."Int Arch Allergy Immunol.. 131 Suppl 1. 15-19 (2003)
Description
「研究成果報告書概要(欧文)」より
-
-
-
-
-
-
-
-
-
