2003 Fiscal Year Final Research Report Summary
精神分裂病関連遺伝子操作動物の作製とその行動および脳内分子の解析
| Project/Area Number |
14570913
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
KASHIWA Astushi Tokyo Medical and Dental University Graduate School, RESEARCH ASSOCIATE, 大学院・医歯学総合研究科, 助手 (10301227)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIKAWA Toru Tokyo Medical and Dental University Graduate School, Professor, 大学院・医歯学総合研究科, 教授 (00198441)
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| Project Period (FY) |
2002 – 2003
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| Keywords | Schizophrenia / Reverse tolerance / Knockout mouse / Transgenic mouse / mrt-1 / Two-Hybrid / coimmunoprecipitation |
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| Research Abstract |
1). We are establishing the transgenic mice line which express mrt-1b gene excessively in the forebrain area using CaMKTI promoter. Germline gene transmission has been confirmed in two of the four transgenic lines. Now we are breeding the F2 generation of the mice.
2). We have cloned six proteins which interact with Mrt1b using the yeast two-hybrid system. The interactions were confirmed by coimmunoprecipitation method. In this method, HA-tagged Mrt1b and Myc-tagged clones were cotransfected into HEK293 mammalian cells and were coimmunoprecipitated by each antibodies. Four of the six clones were unknown. Among the known genes, one belongs to the phosphatase family and the other has a SAM domain and a PD domain.
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