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2003 Fiscal Year Final Research Report Summary

Does ethanol-induced lipid metabolism dysfunction aggravate symptoms of dementia?

Research Project

Project/Area Number 14570936
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Psychiatric science
Research InstitutionSapporo Medical University

Principal Investigator

IKEDA Hiroshi  Sapporo Med.Univ.Sch.Med., Dept.Neuropsychiatry, Assistant Prof., 医学部, 講師 (30232193)

Co-Investigator(Kenkyū-buntansha) YOSHIDA Taku  Sapporo Med.Univ.Sch.Med., Dept.Neuropsychiatry, Instructor, 医学部, 助手 (60347169)
YAMAMOTO Megumi  Sapporo Med.Univ.Sch.Med., Dept.Neuropsychiatry, Assistant Prof., 医学部, 講師 (90347170)
SAITO Toshikazu  Sapporo Med.Univ.Sch.Med., Dept.Neuropsychiatry, Professor, 医学部, 教授 (50128518)
Project Period (FY) 2002 – 2003
Keywordsalcohol dependence / Alzheimer's disease / annexin IV / CREB / NF κ-B / BDNF / ApoE
Research Abstract

Ethanol has been shown to induce apoptosis in normal tissues including neural tissues. It has also reported that ethanol has an aspect of aggravate symptoms of dementia. Our previous studies suggest that the quantitative reduction of certain type of adenylyl cyclase is related to the features of alcohol dependence and Alzheimer's disease indicating that cAMP-mediated signal transduction is disordered in those brains. In the present study, we measured the ethanol-induced cytotoxicity and the quantitative alteration of brain-derived neurotrophic factor(BDNF), which is one of the important target genes of CREB. Using SH-SY5Y cells, it was revealed that the amount of BDNF was decreased by the exposure to ethanol in the cultured cells, whereas the activity of NF κ-B was increased. We next investigated the effects of ethanol on the survival on primary cultured rat cortical neurons and the differentiation of neural stem cells(NSCs). Ethanol exposure decreased the survival of neurons. Both ins … More ulin-like growth factor-1(IGF-1) and BDNF promoted neuronal differentiation of NSCs in a dose-dependent-manner, and the inhibition of NSC differentiation by ethanol was suppressed by IGF-1 and BDNF. Ethanol may inhibit NSC differentiation through alteration of cellular pathways related to neurotrophic factors. Furthermore, the isoform specific effects of apoE on the ethanol-induced cytotoxicity were identified. It was shown that the addition of apoE4 diminish the ethanol-induced cytotoxicity, whereas the presence of neither apoE2 nor apoE3 inhibits the cytotoxicity by the exposure of ethanol. The precise mechanisms of promoting effect of apoE on ethanol-induced cell damage have yet to be clarified and further investigations will be required. However, the present study suggests that the ethanol-induced alterations of second messengers below receptors effect on intracellular transfactors and alternate structure of neural network through expression of genes and intracellular proteins, contributing to the development of cognitive disturbances. To the contrary, induction of differentiation into neurons, recovery of number of neurons and promotion of remodeling of neural network may be applied to the treatment of alcoholism and Alzheimer's disease. It seems possible to broaden our choices of therapeutic strategies by approaching to those illnesses regarding them as a cognitive disorders caused by dysfunctions of lipid metabolism and neural network system. Less

  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Tateno M: "Ethanol inhibition of neural stem cell differentiation is reduced by neurotrophic factors."Alcohol Clin Exp Res. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 館農 勝: "エタノールによる神経幹細胞内シグナル変化と分化制御"アルコールと医学生物学. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hashimoto E: "Impairment of Gs (alpha) function in human cortex of Alzheimer's disease : comparison with normal aging."J Neural Transm. 111. 311-322 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山本 恵: "エタノールによる脳内神経回路網改変の可能性"日本アルコール・薬物医学雑誌. 39. 51-60 (2004)

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      「研究成果報告書概要(和文)」より
  • [Publications] 齋藤利和: "アルコール依存の診断と治療"精神神経学雑誌. 107. 102-107 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 館農 勝: "神経幹細胞の分化誘導抑制に対するエタノールの影響"アルコールと医学生物学. 23. 115-120 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sohma H: "Augmentation of ethanol-induced cell damage and activation of nuclear factor-kappa B by annexin IV in cultured cells."Alcohol Clin Exp Res. 27. 64-67 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 池田官司: "アルコール依存症の診断と概念の変遷"日本アルコール精神医学雑誌. 9. 19-28 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 相馬 仁: "アルコールによる細胞内Ca2+情報伝達素子の発現変化と細胞障害性"アルコールと医学生物学. 22. 4-9 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 齋藤利和: "アルコール依存の生物学的研究とその臨床的応用"精神神経学雑誌. 104. 191-200 (2002)

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      「研究成果報告書概要(和文)」より
  • [Publications] Ohkawa H: "Ethanol-induced augmentation of annexin IV in cultured cells and the enhancemant of cytotoxicity by oversxpression of annexin IV by ethanol"Blochim Biophys Acta. 1488. 217-225 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sohma H: "Ethanol-induced augmentation of annexin IV expression in rat C6 glicoma and human A549 adenocarcinoma cells."Alcohol Clin Exp Res. 26. 44-48 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 池田官司: "別冊日本臨床 領域別症候群40 精神医学症候群III-器質・症状性精神障害など-アルコール幻覚症"日本臨床社. 4 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 池田官司: "別冊日本臨床 領域別症候群40 精神医学症候群III-器質・症状性精神障害など-単純酩酊"日本臨床社. 4 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohkawa H. et al.: "Ethanol-induced augmentation of annexin IV in cultured cells and the enhancemant of cytotoxicity by oversxpression of annexin IV by ethanol."Biochim Biophys Acta. 1488. 217-225 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sohama H. et al.: "Ethanol-induced augmentation of annexin IV expression in rat C6 glioma and human A549 adenocarcinoma cells."Alcohol Clin Exp Res. 26. 44-48 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikeda H. et al.: "Historical perspective on diagnosis and disease concept of alcohol dependence."Jpn J psychiatric research on alcoholism(Japanese). 9. 19-28 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sohama H. et al.: "Ethanol-induced alteration of the expressive amount of the Ca^<2+>-signal transducer involved in cytotoxicity."Alcohol and Biomedical Research(Japanese). 22. 4-9 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito T. et al.: "Biological Research on alcohol dependence."Psychatria Neurologia Japonica(Japanese). 104. 191-200 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sohama H. et al.: "Augmentation of ethanol-induced cell damage and activation of nuclear factor-kappa B by annexin IV in cultured cells."Alcohol Clin Exp Res. 27. 64-67 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tateno M. et al.: "The effects of ethanol on neural stem cell differentiation."Alcohol and Biomedical Research(Japanese). 23. 115-200 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hashimoto E. et al.: "Impairment of Gs(alpha) function in human cortex of Alzheimer's disease : comparison with normal aging."J Neural Transm. 111. 311-322 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito T. et al.: "Diagnosis and therapy of alcohol dependence."Psychatria Neurologia Japonica(Japanese). 106. 102-107 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamamoto M et al.: "Possible alterations in brain neural network by ethanol."Jpn J Alcohol & Drug Dependence. 39. 51-60 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tateno M. et al.: "Ethanol inhibition of neural stem cell differentiation is reduced by neurotrophic factors."Alcohol Clin Exp Res. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tateno M. et al.: "Ethanol-induced alterations on cellular signal transduction and ethanol inhibition of neural stem cell differentiation."Alcohol and Biomedical Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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