2003 Fiscal Year Final Research Report Summary
Identification of novel molecules associated for graft-versus-myeloma effect by SEREX screening
| Project/Area Number |
14570961
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | CHIBA UNIVERSITY |
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Principal Investigator |
NAKASEKO Chiaki CHIBA UNIVERSITY, UNIVERSITY HOSPITAL, ASSISTANT, 医学部附属病院, 助手 (30323398)
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| Co-Investigator(Kenkyū-buntansha) |
NISHIMURA Miki CHIBA UNIVERSITY, UNIVERSITY HOSPITAL, LECTURER, 医学部附属病院, 講師 (10292690)
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| Project Period (FY) |
2002 – 2003
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| Keywords | multiple myeloma / graft versus myeloma effect / graft-versus-host disease / allogeneic stem cell transplantation / SEREX / TRAP1 / RRS-1 |
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| Research Abstract |
To identify the molecules associated with graft-versus-myeloma (GVM) effect, we screened phage cDNA library of myeloma cell line, RPMI8226 by SEREX immuno-screening. The sera used for the screening was the patient's sera 1 year post allogeneic non-myeloablative stem cell transplantation who obtained complete remission by GVM effect after transplantation. Twenty-eight clones were obtained and after in vivo excision, DNA sequence was performed. Nine genes were identified and among them, TNF-α receptor associated protein 1 (TRAP1) gene had 15 clones and RRS-1 gene had 5 clones. Five genes were unknown genes. The expression of TRAP 1 and RRS-1 were higher in various myeloma cell lines than in myeloid leukemia cell lines. These molecules were potential candidate for immuno-targeting therapy for multiple myeloma.
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