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2004 Fiscal Year Final Research Report Summary

Cloning and characterization of a novel coactivator, ANT-1, for androgen receptor AF-1 domain

Research Project

Project/Area Number 14571068
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Endocrinology
Research InstitutionKyushu University

Principal Investigator

GOTO Kiminobu  KYUSHU UNIVERSITY, University Hospital, Research Associate, 大学病院, 助手 (90284512)

Co-Investigator(Kenkyū-buntansha) YANASE Toshihiko  KYUSHU UNIVERSITY, Graduate School of Medical Sciences, Associate Professor, 医学研究院, 助教授 (30239818)
NAWATA Hajime  KYUSHU UNIVERSITY, Graduate School of Medical Sciences, Professor, 医学研究院, 教授 (10038820)
Project Period (FY) 2002 – 2004
Keywordsandrogen receptor / transcriptional coactivator / activation function-1 / confocal microscopy / nuclear localization signal / subnuclear compartment / mammalian two-hybrid
Research Abstract

We have previously identified a novel coactivator for the activation function-1 (AF-1) domain of the androgen receptor (AR). This coactivator, named androgen receptor N-terminal domain transactivating protein-1 (ANT-1), is unique, since the ANT-1 is a component of U5 small nuclear ribonucleoparticle (snRNP), thus raising the possibility that the AR-AF-1 may be involved in the transcription-splicing coupling via ANT-1. We identified four distinguished functional domains required for ANT-1 to play a role as a unique coactivator for AR ; which were transactivating domain, AR-AF-1 binding domain, nuclear translocation signal domain, and intranuclear speckle formation domain. In the ANT-1, consisted of 941 amino acid (aa) residues, about two thirds of the molecule from 291 aa to the C-terminal end contains 19 tetratricopeptide repeat (TPR) motifs which has been speculated to play a role in protein-protein interactions. Interestingly both AR-AF-1 binding domain and the speckle formation domain of ANT-1 were assigned within this long region containing multiple TPR motifs, while transactivating domain and the nuclear translocation signal resided only in the 1 to 172 aa residues. Furthermore, the transactivating domain of ANT-1 was not specific for the AR, that is it enhanced even non-specific viral prompters. The receptor specificity of ANT-1 transactivation was simply determined by the TPR containing region. These results indicated that the ANT-1 actually contained four distinguished intramolecular functional domains, thus suggesting that the ANT-1 play a significant role in vivo.

  • Research Products

    (8 results)

All 2006 2005 2004 2003 2002

All Journal Article (8 results)

  • [Journal Article] Identification of the functional domains of ANT-1, a novel coactivator of the androgen receptor2006

    • Author(s)
      Fan S, et al.
    • Journal Title

      Biochem Biophys Res Commun. 341

      Pages: 192-201

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Identification of the functional domains of ANT-1, a novel coactivator of the androgen receptor.2006

    • Author(s)
      Fan S, et al.
    • Journal Title

      Biochem Biophys Res Commun. 341

      Pages: 192-201

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] Dehydroepiandrosterone negatively regulates the p38 mitogen-activated protein kinase pathway by a novel mitogen-activated protein kinase phosphatase2005

    • Author(s)
      Ashida K. et al.
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression E-pub Feb 23

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Dehydroepiandrosterone negatively regulates the p38 mitogen-activated protein kinase pathway by a novel mitogen-activated protein kinase phosphatase.2005

    • Author(s)
      Ashida K. et al.
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - GeneStructure and Expression 1728

      Pages: 84-94

    • Description
      「研究成果報告書概要(欧文)」より
  • [Journal Article] SF-1/Ad4BP transforms primary long-term cultured bone marrow cells into ACTH-responsive steroidogenic cells.2004

    • Author(s)
      Gondo S. et al.
    • Journal Title

      Genes Cells 9

      Pages: 1239-1247

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Activation function-1 domain of androgen receptor contributes to the interaction between two distinct subnuclear compartments.2003

    • Author(s)
      Goto K.et al.
    • Journal Title

      J Steroid Biochem Mol Biol. 85

      Pages: 201-208

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Activation function-1 domain of androgen receptor contributes to the interaction between subnuclear splicing factor compartment and nuclear receptor compartment. Identification of the p102 U5 small nuclear ribonucleoprotein particle-binding protein as a coactive-ator for the receptor2002

    • Author(s)
      Zhao Y.et al.
    • Journal Title

      J Biol Chem 277

      Pages: 30031-30039

    • Description
      「研究成果報告書概要(和文)」より
  • [Journal Article] Activation function-1 domain of androgen receptor contributes to the interaction between subnuclear splicing factor compartment and nuclear receptor compartment.2002

    • Author(s)
      Zhao Y.et al.
    • Journal Title

      J Biol Chem 277

      Pages: 30031-30039

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2007-12-13  

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