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2003 Fiscal Year Final Research Report Summary

Identification of molecules implicated in insulin response of glucose transport in skeletal muscle and adinose tissue

Research Project

Project/Area Number 14571091
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

INOUE Gen  Kyoto University, Graduate School of Medicine, Assistant Professor, 医学研究科, 講師 (20260606)

Co-Investigator(Kenkyū-buntansha) HOSODA Kiminori  Kyoto University, Graduate School of Medicine, Assistant Professor, 医学研究科, 助手 (40271598)
Project Period (FY) 2002 – 2003
Keywordsinsulin responsiveness / adipocyte / DNA chip / transcriptional factor / insulin responsiveness / GLUT4
Research Abstract

We established conditions for the induction of insulin sensitivity such as localization of GLUT4, induction of intracellular vesicle and enhancement of GLUT4 translocation in adipocyte differentiation using 3T3-L1 cells and 3T3-C2 cells. We examined changes of gene expression in each steps of adipocyte differentiation using DNA chips from Affmetrix company Using this technique, we identified genes the expression of which was changed on induction of high insulin sensitivity. We identified approximately 100-150 known genes the expression of which were increased or decreased. We found approximately 200-300 ESTs the expression of which were increased Furthermore, on the basis of these gene expression profiles, we picked up the genes implicated in intracellular signal transduction or transcriptional regulation. We checked the expression of the genes using Western blot or Northern blot in rat skeletal muscle. We identified one transcriptional factor the expression of which was increased on induction of insulin sensitivity in 3T3-L1 cells and in 3T3-C2 cells. This transcriptional factor was expressed also in skeletal muscle. This factor plays an important role in lipid metabolism, and is implicated in regulation of a molecule in GLUT4 vesicle. This molecule may connect lipid metabolism and carbohydrate metabolism in adipocyte. We are engaged in elucidation of physiological role of the molecule. We confirmed the change of expression of other genes which are implicated in inflammation, in GTP-binding protein, in signal transduction, extracellular matrix. We now examine whether these the expression of these genes are regulated by known transcriptional regulation in adipocyte differentiation. We also investigate mechanism of transcriptional regulation controlling adipocyte differentiation which may implicated GLUT4 translocation.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] T.Miyawaki, et al.: "Clinical implications of leptin and its potential humoral regulators in long-term low-calorie diet therapy for obese humans."Eur.J.Clin.Nutr.. 56(7). 593-600 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Iwakura, et al.: "Ghrelin expression in islet cell tumors : augmented expression of ghrelin in a case of glucagonoma with multiple endocrine neoplasm type I."J.Clin.Endocrinol.Metab.. 87(11). 4885-4888 (2002)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Takahashi-Yasuno, G.Inoue, et al.: "Lteptin receptor polymorphism is associated with serum lipid levels and impairment of cholesterol lowering effect by simvastatin in Japanese men."Diabetes Res Clin Pract. 62(3). 169-175 (2003)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] C.Son, K.Hosoda, et al.: "Reduction of diet-induced obesity in mice overexpressing uncoupling protein 3 in skeletal muscle."Diabetologia. 47. 47-54 (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Toyoda, G.Inoue, et al.: "Possible involvement of the {alpha}1 isoform of 5'AMP-activated protein kinase in oxidative-stress-stimulated glucose transport in skeletal muscle."Am J Physiol Endocrinol Metab.. (in press). (2004)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Miyawaki, et al.: "Clinical implications of leptin and its potential humoral regulators in long-term low-calorie diet therapy for obese humans."Eur.J.Gun.Nutr.. 56(7). 593-600 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Iwakura, et al.: "Ghrelin expression in islet cell tumors; augmented expression of ghrelin in a case of glucagonoma with multiple endocrine neoplasm type I."J.Gun.Endocrinol.Metab.. 87(11). 4885-4888 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A.Takahashi-Yasuno, G.Inoue, et al.: "Leptin receptor polymorphism is associated with serum lipid levels and impairment of cholesterol lowering effect by simvastatin in Japanese men."Diabetes Res Gun Pract. 62(3). 169-175 (2003)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] C.Son, K.Hosoda, et al.: "Reduction of diet-induced obesity in mice overexpressing uncoupling protein 3 in skeletal muscle."Diabetologia. 47. 47-54 (2004)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Toyoda, G.Inoue, et al.: "Possible involvement of the {alpha} 1 isoform of 5'AMIP-activated protein kinase in oxidative-stress-stimulated glucose transport in skeletal muscle."Am J Physiol Endocrinod Metab. (in press). (2004)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 2005-04-19  

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